Epidemiology of tuberculosis in Kuwait from 1965 to 1999

Behbehani, Nasser; Abal, A.T.; A, Al-Shami; Da, Enarson

doi:10.5588/09640569512940

Cited by 18 publications

(31 citation statements)

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“…Recent data from Kuwait, another Arabian country in the Persian Gulf region which also has a large expatriate population originating from TB endemic countries of South Asia and Southeast Asia, have also shown that the majority of isoniazid-resistant M. tuberculosis infections occur in the expatriate population [11,19]. Further, similar to the data reported here, most of the isoniazid-resistant M. tuberculosis strains from Kuwait also exhibited unique patterns in typing studies [5,11].…”

Section: Discussionsupporting

confidence: 73%

Genotypic Diversity among Isoniazid-Resistant Isolates of Mycobacterium tuberculosis from Rashid Hospital in Dubai, United Arab Emirates

Ahmad

Fares²

2004

Med Princ Pract

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Objective: To perform molecular fingerprinting for strain relatedness among isoniazid-resistant Mycobacterium tuberculosis isolates recovered from tuberculosis (TB) patients in the United Arab Emirates (UAE). Materials and Methods: Thirty-two consecutive isoniazid-resistant M. tuberculosis strains isolated from TB patients (4 natives and 28 expatriates) at Rashid Hospital, Dubai, UAE were analyzed. The typing was carried out by touchdown double-repetitive-element PCR (DRE-PCR). The status of R463 or L463 polymorphism and the presence of S315T mutation in the katG gene were also determined for isolates exhibiting cluster pattern in DRE-PCR. Results: All the 32 isolates (28 from expatriate patients and 4 from UAE nationals) exhibited 21 distinct patterns in DRE-PCR with 20 of 32 isolates exhibiting unique patterns and the remaining 12 exhibiting cluster ‘A’ pattern. All the isolates (19 of 19) yielding two or more DNA fragments in DRE-PCR were unique strains. The genotypic heterogeneity among 10 of the 12 cluster isolates was suggested by the varying susceptibility of these isolates to anti-TB drugs, presence of R463 or L463 polymorphism in the katG gene and the presence or absence of S315T mutation in the katG gene. Conclusion: The genotypic diversity among isoniazid-resistant M. tuberculosis isolates mostly recovered from expatriate patients indicates that most expatriates were infected with a unique strain imported, most likely, from their country of origin and that their latent infection was reactivated in UAE.

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Section: Discussionsupporting

confidence: 73%

Genotypic Diversity among Isoniazid-Resistant Isolates of Mycobacterium tuberculosis from Rashid Hospital in Dubai, United Arab Emirates

Ahmad

Fares²

2004

Med Princ Pract

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“…Kuwait, an Arabian country in the Persian Gulf region with nearly 25 cases per 100 000 population, has a low incidence of TB [23]. However, Kuwait has a large expatriate workforce originating from TB endemic countries.…”

Section: Introductionmentioning

confidence: 98%

The occurrence of rare mutations in rifampicin-resistant clinical isolates from Kuwait

Ahmad

Mokaddas

2005

International Journal of Antimicrobial Agents

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“…The recent estimates suggest that about one third of the world population is infected with Mycobacterium tuberculosis , 8-10 million people develop active disease and 2 million people die of TB each year [1] . In addition to being an international problem, TB is also a serious infectious disease problem in the countries of the Gulf Region, including Kuwait [2] . The effective control of TB is dependent on the availability of universally effi cacious vaccines and reagents for specifi c diagnosis [3] .…”

Section: Introductionmentioning

confidence: 99%

HLA-DR Binding Prediction and Experimental Evaluation of T-Cell Epitopes of Mycolyl Transferase 85B (Ag85B), a Major Secreted Antigen of Mycobacterium tuberculosis

Mustafa

Abal²,

Shaban³

et al. 2005

Med Princ Pract

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Objective: To identify T-cell epitopes of Ag85B by analysis of its sequence for prediction to bind HLA-DR alleles and evaluate the predicted peptides for recognition by T cells in antigen-induced proliferation assays. Materials/Subjects and Methods: The complete sequence of Ag85B was analyzed for HLA-DR binding prediction to 51 HLA-DR alleles by using a virtual matrix-based prediction program (ProPred). Synthetic peptides covering the sequence of mature Ag85B were also analyzed for binding to HLA-DR alleles, and evaluated for recognition in antigen-induced proliferation assays with Ag85B-specific T-cell lines established from the peripheral blood mononuclear cells of 10 HLA-DR-heterogeneous tuberculosis patients. Results: The ProPred analysis of the full-length Ag85B (325 aa), signal peptide (40 aa) and the mature protein (285 aa) predicted their binding to 100, 76 and 98% of the 51 HLA-DR alleles, respectively. The analysis of 31 synthetic peptides for binding to HLA-DR alleles showed that 4 of them could bind >50% HLA-DR alleles, and were considered promiscuous. Testing of Ag85B-specific T-cell lines with synthetic peptides showed that all of the T-cell lines responded to one or more peptides of Ag85B, and 9 of the 10 cell lines responded to one or more of the four peptides considered promiscuous for binding to HLA-DR alleles. Conclusion: The ProPred program was useful in predicting the HLA-DR alleles binding regions of Ag85B and identifying the promiscuous peptides recognized by T cells.

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Epidemiology of tuberculosis in Kuwait from 1965 to 1999

Abstract: Tuberculosis in Kuwait declined steadily from 1965 to 1989. Since 1989 there has been a slight rise in TB incidence in Kuwait among both Kuwaiti nationals and non-nationals.

Cited by 18 publications

References 0 publications

Genotypic Diversity among Isoniazid-Resistant Isolates of <i>Mycobacterium tuberculosis</i> from Rashid Hospital in Dubai, United Arab Emirates

Genotypic Diversity among Isoniazid-Resistant Isolates of <i>Mycobacterium tuberculosis</i> from Rashid Hospital in Dubai, United Arab Emirates

The occurrence of rare mutations in rifampicin-resistant clinical isolates from Kuwait

HLA-DR Binding Prediction and Experimental Evaluation of T-Cell Epitopes of Mycolyl Transferase 85B (Ag85B), a Major Secreted Antigen of <i>Mycobacterium tuberculosis</i>

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