Epidemiology of the Acinetobacter-derived cephalosporinase, carbapenem-hydrolysing oxacillinase and metallo-β-lactamase genes, and of common insertion sequences, in epidemic clones of Acinetobacter baumannii from Spain

Villalón, Pilar; Valdezate, Sylvia; Medina, María José; Carrasco, Gema; Vindel, Ana; Sáez-Nieto, Juan Antonio

doi:10.1093/jac/dks448

Cited by 70 publications

(53 citation statements)

References 16 publications

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“…Two strains with a negative CarbAcineto NP test probably gave a false-positive result in the inhibitor-based test. Similar observation of the false-positive MBL test in strains positive for CHDL was previously reported by Vilalon et al 65 The possible explanation is that, in the presence of EDTA, oxacillinase changes to a less active state, leading to a drastic reduction in MIC or augmentation of the inhibition zone. 6 OXA-23 b-lactamase was found in a majority of isolates combined with VIM MBL.…”

Section: Discussionsupporting

confidence: 85%

Nursing Home as a Reservoir of Carbapenem-ResistantAcinetobacter baumannii

Bedenić

Beader

Godič-Torkar

et al. 2015

Microbial Drug Resistance

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Acinetobacter baumannii is an increasingly common pathogen in healthcare settings globally. It is frequently resistant to multiple antimicrobial agents and there are recent reports on strains that are pandrug resistant. The aim of the study was to characterize the mechanisms of carbapenem resistance of A. baumannii strains from a nursing home in Zagreb and to genotype the strains. Antimicrobial susceptibility testing was performed by the broth microdilution method according to the Clinical and Laboratory Standards Institute (CLSI). PCR was used to detect genes encoding carbapenemases of groups A, B, and D and extended-spectrum β-lactamases. Genotyping of the strains was performed by rep-PCR. All strains were found to be resistant to ceftazidime, cefotaxime, ceftriaxone, cefepime, piperacillin/tazobactam, imipenem, meropenem, and ciprofloxacin. All, but one strain, were resistant to gentamicin. PCR revealed blaOXA-23 genes in 14, blaOXA-24 in 5, and blaVIM in 11 strains. All strains positive for blaVIM genes coharbored blaOXA-23 genes. The 14 strains with OXA-23 belonged to ICL II, whereas the 5 strains positive for blaOXA-24 belonged to ICL I. In contrast to hospitals where OXA-24/40-like β-lactamases and OXA-58 were the most prevalent, OXA-23-like β-lactamases are the dominant group in the nursing home. OXA-58-like β-lactamase, which is the most widespread group, was not found. Acquisition of blaMBL genes in A. baumannii strains was observed. Rep-PCR identified two clones. Two strains A10 and A13 were alocated to a novel sequence type ST 637. Nursing homes can act as a source of dissemination of blaOXA and blaMBL genes in the environment and the possible influx to the hospital environment.

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Section: Discussionsupporting

confidence: 85%

Nursing Home as a Reservoir of Carbapenem-ResistantAcinetobacter baumannii

Bedenić

Beader

Godič-Torkar

et al. 2015

Microbial Drug Resistance

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“…The prevalence of imipenem resistance among A. baumannii in 2012 was 88%. This number is close to reported rates equivalent to 70% in Egypt (24), 72% in Turkey (25), 75% in Spain (26), and up to 100% in Italy (27).…”

Section: Discussionsupporting

confidence: 78%

Spread of imipenem-resistant Acinetobacter baumannii co-expressing OXA-23 and GES-11 carbapenemases in Lebanon

Halat

Moubareck

Hakime

et al. 2015

International Journal of Infectious Diseases

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“…However, they are detected at very high frequencies in southern European Mediterranean countries such as Italy (130), Greece (131), and Turkey (132). Until recently, the bla OXA-40-like genes appeared to be restricted largely to the Iberian Peninsula, where they have been the predominant acquired OXA-type ␤-lactamase (133)(134)(135). However, reports have identified bla OXA-40-like genes in more distant locations, such as the United States (136) and Asia (31), and in particular, there are increasingly reports of these enzymes being identified on plasmids in Taiwan (137,138).…”

Section: Geographical Disseminationmentioning

confidence: 99%

OXA β-Lactamases

2014

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SUMMARY The OXA β-lactamases were among the earliest β-lactamases detected; however, these molecular class D β-lactamases were originally relatively rare and always plasmid mediated. They had a substrate profile limited to the penicillins, but some became able to confer resistance to cephalosporins. From the 1980s onwards, isolates of Acinetobacter baumannii that were resistant to the carbapenems emerged, manifested by plasmid-encoded β-lactamases (OXA-23, OXA-40, and OXA-58) categorized as OXA enzymes because of their sequence similarity to earlier OXA β-lactamases. It was soon found that every A. baumannii strain possessed a chromosomally encoded OXA β-lactamase (OXA-51-like), some of which could confer resistance to carbapenems when the genetic environment around the gene promoted its expression. Similarly, Acinetobacter species closely related to A. baumannii also possessed their own chromosomally encoded OXA β-lactamases; some could be transferred to A. baumannii , and they formed the basis of transferable carbapenem resistance in this species. In some cases, the carbapenem-resistant OXA β-lactamases (OXA-48) have migrated into the Enterobacteriaceae and are becoming a significant cause of carbapenem resistance. The emergence of OXA enzymes that can confer resistance to carbapenems, particularly in A. baumannii , has transformed these β-lactamases from a minor hindrance into a major problem set to demote the clinical efficacy of the carbapenems.

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Epidemiology of the Acinetobacter-derived cephalosporinase, carbapenem-hydrolysing oxacillinase and metallo-β-lactamase genes, and of common insertion sequences, in epidemic clones of Acinetobacter baumannii from Spain

Abstract: All the studied genes and their related ISs showed a clonal distribution. Imipenem resistance was probably provided by OXA-40 for the most part, while MBL- and OXA-23-encoding genes were absent in the studied population.

Cited by 70 publications

References 16 publications

Nursing Home as a Reservoir of Carbapenem-ResistantAcinetobacter baumannii

Nursing Home as a Reservoir of Carbapenem-ResistantAcinetobacter baumannii

Spread of imipenem-resistant Acinetobacter baumannii co-expressing OXA-23 and GES-11 carbapenemases in Lebanon

OXA β-Lactamases

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