Epidemiology of Staphylococcus pseudintermedius in cats in Poland

Abstract

Staphylococcus pseudintermedius is a well-known coagulase-positive staphylococcus that is mainly associated with the asymptomatic colonization of the skin of pets and mucous membranes. Little is still known about the occurrence of S. pseudintermedius in cats. The current study aimed to characterize the isolates of S. pseudintermedius from sick and healthy cats. This was achieved by examining their antibiotic resistance properties, biofilm formation, and genotype differences. Six hundred and seventy-six cats were swabbed (595 healthy and 81 sick cats). Thirty-five distinct S. pseudintermedius isolates from 27 cats were isolated. The prevalence of S. pseudintermedius in healthy and sick cats was 2.49% and 7.61%, respectively. In comparison, MRSP (methicillin-resistant Staphylococcus pseudintermedius) prevalence was 0.12% and 2.98%, respectively. Cats were more frequently colonized with S. pseudintermedius when kept with dogs, regardless of their health condition, with this result being statistically significant. Multidrug resistance was detected in 50%, and 38.46% of S. pseudintermedius isolates from healthy and sick cats, respectively. In contrast, genetic multidrug resistance was detected in 59% and 46.15% cases, respectively. Seven from eight isolated MRSPs were multidrug-resistant. Multi-locus sequence typing (MLST) assigned isolates to 19 types, of which 16 types submitted for the first time to the PubMLST database. The most frequently detected STs (sequence types) were 551 and 71. ST71 and ST551 were mainly isolated from cats with clinical signs of infection. All were MRSPs, regardless of cats’ health. These isolates were characterized with the most frequent antibiotic resistance at the phenotypic and genotypic level.

Antimicrobial resistance in Staphylococcus pseudintermedius on the environmental surfaces of a recently constructed veterinary hospital in Southern Thailand

“…Seriously, two colonies of MSSP were obtained from the surgical environment in the present study, although this environment is expected to be sterile to prevent surgical site infections. Staphylococci contamination of the surgical environment is occasionally found in veterinary hospitals and may cause surgical site infections [ 2 , 32 ]. Fortunately, S. pseudintermedius infection was not observed in any of the dogs operated in this hospital (unpublished data).…”

Staphylococcus aureus Causing Skin and Soft Tissue Infections in Companion Animals: Antimicrobial Resistance Profiles and Clonal Lineages

“…Bacterial pyoderma is mainly associated with coagulase-positive staphylococci. In dogs, S. aureus may be responsible for up to 10% of pyoderma cases [2], whereas in cats, it is often associated with a higher proportion of cases [4,24]. This difference in the frequency rate of S. aureus between cats and dogs may reflect the lower adherence of S. pseudintermedius to feline corneocytes when compared to canine corneocytes [25].…”

“…In dogs, Staphylococcus pseudintermedius accounts for nearly 90% of pyoderma cases, whereas Staphylococcus aureus and Staphylococcus coagulans are interchangeably the second most common pathogen accounting for up to 10% of pyoderma cases [2,3]. In cats, S. aureus is a more frequent agent of pyoderma than S. pseudintermedius, although there is less available literature for these cases [4]. The prevalence rate of these bacterial pathogens in other companion animals, such as rabbits, is relatively unknown.…”

Prevalence of Bacterial Species in Skin, Urine, Diarrheal Stool, and Respiratory Samples in Cats

“…In the present study, our findings were consistent with those of a previous study by Polish researchers. Bierowiec et al reported that S. pseudintermedius is the least prevalent bacteria in cats among the Staphylococcus class of bacteria [ 9 ]. In previous reports, the prevalence of S. pseudintermedius in dogs was 90%, which is higher than that in cats with a prevalence range of 2.49–8.8% [ 10 , 11 , 12 , 13 , 14 , 15 ].…”