This study was aimed at screening for non-treponemal antibodies, anti-hepatitis C and hepatitis B surface antigen markers among asymptomatic students of Western Delta University, Oghara, Nigeria. Venous blood was collected from 318 students of 26 years average age who did not show any visible signs or symptoms of any of the three infections. Blood samples were processed and screened for hepatitis C, hepatitis B and syphilis by rapid test methods. Blood samples were collected and processed in the Microbiology and Biotechnology Laboratory of Western Delta University, Nigeria between June 2013 and October, 2013. Anti-hepatitis C assay was done by a one step hepatitis C virus test strip, hepatitis B assay was done by a one step hepatitis B surface antigen test strip and non-treponemal antibodies was done by syphilis ultra rapid test strip. Ninety three (29.3%), 36 (11.3%), 6 (1.9%), 3 (0.9%) and 0.0% number of students belonged to 21-25 (24 years average), 15-20 (19 years average), 26-30 (27 years average), 36-40 (40 years average), 41-45 (44 years average) and 31-35 (30 years average) age groups respectively. Nine (5.1%) male students out of the 117 (55.7%) screened in the 21-25 age bracket tested positive to hepatitis B (produced antibodies against HBsAg) while 0.3 (1.7%) female students of the same age group produced antibodies to Treponema palladium antigen (tested positive to syphilis). Seropositivity to HBsAg was also recorded by 3 (8.3%) male students out of 36 (11.3%) screened in the 26-30 years age group. On the whole, 12 (3.8%) male and 3 (0.9%) female students tested positive to hepatitis B and syphilis, respectively. Hepatitis due to HBV and syphilis infection are prevalent within the university under study and indeed, Oghara town though in apparent low occurrence. The relevant health authorities should ensure a constant sero-epidemiological surveillance as well as institute counseling and vaccination programmes.