Epidemiology of candidemia in Qatar, the Middle East: performance of MALDI-TOF MS for the identification of Candida species, species distribution, outcome, and susceptibility pattern

Taj-Aldeen, Saad J.; Kolecka, Anna; Boesten, R.; Alolaqi, A.; Almaslamani, Muna; Chandra, Prem; Meis, Jacques F.; Boekhout, Teun

doi:10.1007/s15010-013-0570-4

Cited by 60 publications

(49 citation statements)

References 35 publications

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“…This observation is uncommon compared with almost all the previously reported studies that indicated some degree of sensitivity to amphotericin B. Although, in a study conducted by Ruan et al (2008), the resistance was extremely high against amphotericin B in C. krusei strains (95%) and in C. glabrata strains (53%), in recent years, the low rates of amphotericin B resistance have been reported in the C. albicans, C. glabrata, C. parapsilosis, C. tropicalis and C. krusei isolates (Metin et al, 2011;Taj-Aldeen et al, 2014).…”

Section: Discussionmentioning

confidence: 81%

Species distribution and antifungal susceptibility profile of <i>Candida spp</i> isolated from urine of hospitalized patients in Dschang District Hospital, Cameroon

Ekpo¹,

Kechia²,

Iwewe³

et al. 2017

Int. J. Bio. Chem. Sci

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Candiduria refers to the presence of Candida species in urine, It is a common nosocomial infection afflicting the urinary tract. The aim of our study was to determine the prevalence of candiduria in hospitalized patients in Dschang District Hospital, and to evaluate the susceptibility patterns of Candida spp isolated from some commonly used antifungal drugs. Midstream urine samples were collected from 122 hospitalized patients at the Dschang District Hospital, then speciation of Candida was done based on colony colour on CHROMagar Candida medium. Antifungal susceptibility testing of the isolates was performed by the Broth dilution method. A total of 51 isolates of Candida species were recovered from 122 urine samples. Candida albicans was the most prevalent species 41.2% (21 isolates), followed by C. parapsilosis (27.5%), C. glabrata (17.6%), C. krusei (7.8%) and C. tropicalis (5.9%). Antifungal susceptibility test indicated that 100% and 96.1% of the Candida isolates were resistant to Amphotericin B and Nystatin respectively. Resistance varied depending on the species and the respective antifungal agents. We can conclude that candiduria is relatively common in hospitalized patients. The isolates were very resistant to polyenes (amphotericin B and nystatin) and most isolates were sensitive to azoles. Therefore, the species identification of Candida isolates along with their antifungal susceptibility pattern can help the clinicians in adequate treatment of the patients with candiduria.

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Section: Discussionmentioning

confidence: 81%

Species distribution and antifungal susceptibility profile of <i>Candida spp</i> isolated from urine of hospitalized patients in Dschang District Hospital, Cameroon

Ekpo¹,

Kechia²,

Iwewe³

et al. 2017

Int. J. Bio. Chem. Sci

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“…Currently, there is a lack of data on isavuconazole susceptibility patterns among clinical Candida isolates. In an Egyptian epidemiology study, all strains of Candida isolated from 187 patients were susceptible to isavuconazole (range, Ͻ 0.016 to 1 g/ml), while overall resistance to voriconazole was 2.5% in all strains (49). Another study demonstrated isavuconazole to be highly active against 296 Candida bloodstream isolates; the activity of isavuconazole was more potent than that of fluconazole against all organisms tested, and often it was more potent than that of itraconazole or voriconazole (20).…”

Section: Discussionmentioning

confidence: 99%

Activity of Isavuconazole and Other Azoles against Candida Clinical Isolates and Yeast Model Systems with Known Azole Resistance Mechanisms

Sanglard¹,

Coste²

2016

Antimicrob Agents Chemother

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Isavuconazole is a novel, broad-spectrum, antifungal azole. In order to evaluate its interactions with known azole resistance mechanisms, isavuconazole susceptibility among different yeast models and clinical isolates expressing characterized azole resistance mechanisms was tested and compared to those of fluconazole, itraconazole, posaconazole, and voriconazole. Saccharomyces cerevisiae expressing the Candida albicans and C. glabrata ATP binding cassette (ABC) transporters (CDR1, CDR2, and CgCDR1), major facilitator (MDR1), and lanosterol 14-␣-sterol-demethylase (ERG11) alleles with mutations were used. In addition, pairs of C. albicans and C. glabrata strains from matched clinical isolates with known azole resistance mechanisms were investigated. The expression of ABC transporters increased all azole MICs, suggesting that all azoles tested were substrates of ABC transporters. The expression of MDR1 did not increase posaconazole, itraconazole, and isavuconazole MICs. Relative increases of azole MICs (from 4-to 32-fold) were observed for fluconazole, voriconazole, and isavuconazole when at least two mutations were present in the same ERG11 allele. Upon MIC testing of azoles with clinical C. albicans and C. glabrata isolates with known resistance mechanisms, the MIC 90 s of C. albicans for fluconazole, voriconazole, itraconazole, posaconazole, and isavuconazole were 128, 2, 1, 0.5, and 2 g/ml, respectively, while in C. glabrata they were 128, 2, 4, 4, and 16 g/ml, respectively. In conclusion, the effects of azole resistance mechanisms on isavuconazole did not differ significantly from those of other azoles. Resistance mechanisms in yeasts involving ABC transporters and ERG11 decreased the activity of isavuconazole, while MDR1 had limited effect. Invasive fungal infections (IFIs) caused by yeasts are an important cause of morbidity and mortality. Candida spp. are opportunistic fungal pathogens and a particular cause for concern in immunocompromised individuals (1). Candida infections may account for more than 70% of all IFIs (2). Candidemia is a significant problem in the intensive care unit, as it is associated with a crude mortality rate of 42.6% and high resource use (3).Azoles constitute a class of drug commonly used for treating infections caused by Candida spp., and the Infectious Diseases Society of America recommends them as a primary treatment for candidemia in nonneutropenic patients (1). They are fungistatic in most cases and work by binding and inhibiting the enzyme lanosterol 14-␣-sterol-demethylase (encoded by ERG11), which is involved in converting lanosterol into ergosterol (4, 5). As ergosterol is an essential component of the fungal cell membrane, disruption of its biosynthesis inhibits fungal growth.Repeated exposure to azoles and drug pressure in Candida spp. can lead to the emergence of azole-resistant strains, thereby increasing the risk of treatment failure and breakthrough infections (6, 7). Fluconazole resistance is common in many isolates of Candida spp., but they can remain susceptible t...

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“…improved the number of correct identifications at the species level (8). Taj-Aldeen et al (32) showed 100% correct identifications by MALDI-TOF MS of 68 C. albicans isolates and 133 isolates of other Can- dida spp. and uncommon yeasts from bloodstream infections, with scores of Ͼ1.9 using the extended (BDALϩCBS in-house) database used in the present study.…”

Section: Discussionmentioning

confidence: 99%

Interlaboratory Comparison of Sample Preparation Methods, Database Expansions, and Cutoff Values for Identification of Yeasts by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Using a Yeast Test Panel

et al. 2014

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An interlaboratory study using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to determine the identification of clinically important yeasts (n ‫؍‬ 35) was performed at 11 clinical centers, one company, and one reference center using the Bruker Daltonics MALDI Biotyper system. The optimal cutoff for the MALDI-TOF MS score was investigated using receiver operating characteristic (ROC) curve analyses. The percentages of correct identifications were compared for different sample preparation methods and different databases.

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Epidemiology of candidemia in Qatar, the Middle East: performance of MALDI-TOF MS for the identification of Candida species, species distribution, outcome, and susceptibility pattern

Cited by 60 publications

References 35 publications

Species distribution and antifungal susceptibility profile of <i>Candida spp</i> isolated from urine of hospitalized patients in Dschang District Hospital, Cameroon

Species distribution and antifungal susceptibility profile of <i>Candida spp</i> isolated from urine of hospitalized patients in Dschang District Hospital, Cameroon

Activity of Isavuconazole and Other Azoles against Candida Clinical Isolates and Yeast Model Systems with Known Azole Resistance Mechanisms

Interlaboratory Comparison of Sample Preparation Methods, Database Expansions, and Cutoff Values for Identification of Yeasts by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Using a Yeast Test Panel

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