Epidemiology of Babesia, Anaplasma and Trypanosoma species using a new expanded reverse line blot hybridization assay

Paoletta, Martina Soledad; Arias, Ludmila Sol López; Fourniere, Sofía Ana María de la; Guillemi, Eliana Carolina; Luciani, C. A.; Sarmiento, Néstor Fabián; Mosqueda, Juan; Farber, Marisa Diana; Wilkowsky, Silvina Elizabeth

doi:10.1016/j.ttbdis.2017.08.011

Cited by 17 publications

(12 citation statements)

References 35 publications

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“…Anaplasmosis is an endemic disease that affects livestock in Argentina and previous studies have been carried out for the molecular characterization of A. marginale genotypes from cattle located in some of the areas where the studied giant anteaters are found [2,13,14]. In the present study, molecular characterization based on MSP1a tandem repeats analysis revealed the presence of two already known haplotypes.…”

Section: Discussionsupporting

confidence: 51%

Closing the Gaps to Understand the Tick Transmission of Anaplasma marginale among Giant Anteaters (Myrmecophaga tridactyla) in Argentina

et al. 2020

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Anaplasma marginale, a well-known cattle pathogen of tropical and subtropical world regions, has been previously molecularly characterized in a giant anteater (Myrmecophaga tridactyla) from Corrientes, Argentina. Ticks or other hematophagous arthropod involved in the wild transmission cycle remained unknown. The aim of the present study was to analyze the simultaneous occurrence of A. marginale in blood samples and ticks from giant anteaters from Corrientes in order to investigate if ticks could be relevant in the transmission among these mammals. Blood samples from 50 giant anteaters collected in different years and 26 ticks Amblyomma dubitatum and A. sculptum were studied through the molecular amplification of two unequivocal species-specific genes from A. marginale: msp5 and msp1β. Twenty five giant anteaters and tick organs (salivary glands, gut and oviduct) from 11 ticks tested positive to the A. marginale DNA amplification. The further molecular characterization through MSP1a tandem repeats analysis revealed the presence of genotypes circulating among giant anteaters that had been previously identified in cattle blood samples from the same geographical region. These results confirm the presence of A. marginale in giant anteaters in Corrientes and suggests that A. dubitatum and A. sculptum ticks could be involved in the transmission among giant anteaters. Future studies will determine the role of these tick species in the wild transmission cycle in the study area and the eventual connection with the domestic cycle.

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Section: Discussionsupporting

confidence: 51%

Closing the Gaps to Understand the Tick Transmission of Anaplasma marginale among Giant Anteaters (Myrmecophaga tridactyla) in Argentina

et al. 2020

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“…[65], and a fragment of the 18S rRNA gene for Trypanosoma sp. [66] and Babesia/Theileria [67]. For positive samples, both strands of the amplified fragment were sequenced with a Big Dye Terminator v3.1 kit from Applied Biosystems and analysed in an ABI 3130XL genetic analyser from the same supplier (Genomic Unit, Consorcio Argentino de Tecnología Genómica (CATG), Instituto de Biotecnología, CICVyA, INTA).…”

Section: Laboratory Diagnosismentioning

confidence: 99%

A participatory surveillance of marsh deer (Blastocerus dichotomus) morbidity and mortality in Argentina: first results

et al. 2020

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Background: In an era of unprecedented socio-ecological changes, managing wildlife health demands high-quality data collection and the engagement of local communities. Blastocerus dichotomus, the largest South American deer, is Vulnerable to extinction mainly due to habitat loss. Diseases have been recognised as a potential threat, and winter mortality has been historically described in marsh deer populations from Argentina. Field difficulties have, however, prevented in-depth studies of their health status. Results: Between May 2014 and April 2017, we investigated marsh deer morbidity and mortality in the two largest populations in Argentina. We collected data by means of a passive surveillance system that involved a network of researchers, field partners (veterinarians, park rangers, and local community), and decision makers. We sampled marsh deer during as well as outside mortality events. A total of 44 marsh deer with different body condition scores were evaluated. We obtained haematology and biochemistry values from animals with good body condition score. Marsh deer with poor body condition had a high burden of the ticks Amblyomma triste and Rhipicephalus microplus. Vectorborne agents such as Theileria cervi, Trypanosoma theileri, Trypanosoma evansi, Ehrlichia chaffeensis, Anaplasma platys, Anaplasma odocoilei, Anaplasma marginale, and Candidatus Anaplasma boleense were also found. Haemonchus spp., Ostertagia spp., and Trichostrongylus spp. were the most frequent gastrointestinal parasites in deer with poor body condition. A Multiple Correspondence Analysis reinforced a possible association of winter period with lower body score condition, high tick loads, infection with E. chaffeensis, and presence of harmful gastrointestinal parasites.

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“…[65], and a fragment of the 18SrRNA gene for Trypanosoma sp. [66] and Babesia/Theileria [67]. For positive samples, both strands of the ampli ed fragment were sequenced with a Big Dye Terminator v3.1 kit from Applied Biosystems and analysed in an ABI 3130XL genetic analyser from the same supplier (Genomic Unit, Consorcio Argentino de Tecnología Genómica (CATG), Instituto de Biotecnología, CICVyA, INTA).…”

Section: Methodsmentioning

confidence: 99%

A participatory approach to marsh deer (Blastocerus dichotomus) morbidity and mortality passive surveillance in Argentina: first results.

Orozco¹,

Argibay²,

Minatel³

et al. 2020

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Background: The comprehensive approach to diseases in broad ecological contexts appears as a new challenge for global health. Winter mortality of marsh deer ( Blastocerus dichotomus ) was historically described in Argentina even though field difficulties prevented their in-depth study. Results: Between May 2014 and April 2017, we investigated marsh deer morbidity and mortality through passive surveillance in the two largest populations in Argentina using a participatory approach. The latter spanned a network of researchers, field partners (veterinarians, park rangers, and local community), and decision makers. We sampled dead deer during as well as outside mortality events. A total of 44 marsh deer with different body condition scores were evaluated. We obtained reference haematology and biochemistry values from animals with good body condition score. Marsh deer with poor body condition had a high burden of the ticks Amblyomma triste and Rhipicephalus microplus . Vector-borne agents (VBAs), such as Theileria cervi , Trypanosoma theileri, Trypanosoma evansi, Ehrlichia chaffeensis, Anaplasma platys, Anaplasma odocoilei, Anaplasma marginale , and Candidatus Anaplasma boolense were also found. Haemonchus spp., Ostertagia spp., and Trichostrongylus spp. were the most frequent gastrointestinal parasites in deer with poor body condition. A Multiple Correspondence Analysis reinforced a possible association of winter period with lower body score condition, high tick loads, infection with E. chaffeensis , and presence of harmful gastrointestinal parasites. Conclusions: Our approach allowed the establishment of a participatory surveillance network of marsh deer morbidity and mortality in Argentina. We report and analyse the first data obtained opportunistically within the framework of this network, providing information on the infectious and parasitic agents in marsh deer populations. The occurrence of Fasciola hepatica and Leptospira interrogans serovar pyrogenes is reported for the first time in wild marsh deer from Argentina. The histopathological findings in succumbed animals allowed us to distinguish lesions associated with their body condition from those that were unrelated. Our data will be useful to improve the interpretation of future mortality events. The field implementation of a surveillance network is key to a holistic approach to wildlife diseases.

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Epidemiology of Babesia, Anaplasma and Trypanosoma species using a new expanded reverse line blot hybridization assay

Cited by 17 publications

References 35 publications

Closing the Gaps to Understand the Tick Transmission of Anaplasma marginale among Giant Anteaters (Myrmecophaga tridactyla) in Argentina

Closing the Gaps to Understand the Tick Transmission of Anaplasma marginale among Giant Anteaters (Myrmecophaga tridactyla) in Argentina

A participatory surveillance of marsh deer (Blastocerus dichotomus) morbidity and mortality in Argentina: first results

A participatory approach to marsh deer (Blastocerus dichotomus) morbidity and mortality passive surveillance in Argentina: first results.

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