Epidemiology of Actinobacillus Seminis in a Flock of Border Leicester Sheep

Simmons, G. C.; Baynes, I. D.; Ludford, C. G.

doi:10.1111/j.1751-0813.1966.tb04743.x

Cited by 5 publications

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“…These antibodies persisted only up to post-infection day 35. C F titres of 2 1 : 20 were considered positive, while titres of t : 10 were regarded as doubtful for A.seminis infection (1,21,25). Rams with subclinical genital infection did rarely have serum C F titres higher than 1 : 10 (21, 25).…”

Section: Discussionmentioning

confidence: 99%

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Trials with an Enzyme‐Linked Immunosorbent Assay (ELISA) for the Diagnosis of Subclinical Genital Infections in Rams Caused by Histophilus ovis and Actinobacillus seminis

Tekes¹,

Hajtós

1990

Journal of Veterinary Medicine, Series B

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Summary The performance of an enzyme‐linked immunosorbent assay (ELISA) was evaluated in the serological diagnosis of subclinical genital infection in 6 naturally infected ram flocks and 2 experimentally infected ram hoggets. The test employs lipopolysaccharide (LPS) antigen prepared by autoclaving from Actinobacillus seminis and Histophilus ovis. A total of 193 sheep (118 unmated virgin rams and 75 mature breeding rams) were examined clinically, serologically (by ELISA) and bacteriologically (semen bacteriology) at the same time. Serum samples from all animals were also tested by an ELISA employing LPS antigen prepared from Brucella ovis in the same way. Shedding of A. seminis and H. ovis did not show close correlation with serological positivity (Table 1), as only 9 (15.0%) out of the 60 A. seminis shedders were ELISA seropositive at the same time. As regards H. ovis only 10 (19.2 %) out of the 52 H. ovis shedders were ELISA seropositive at the same time. The results indicate that, when used alone, the ELISA employing LPS antigen is unsuitable for diagnosing subclinical genital infection caused by H. ovis and A. seminis in rams. The authors discussed shortly the employing fields of this ELISA test in the diagnostic work.

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Section: Discussionmentioning

confidence: 99%

“…Rams with subclinical genital infection did rarely have serum C F titres higher than 1 : 10 (21, 25). Some rams with no detectable C F titre shed A. seminis in their semen (2, 21). No data had been reported on serum antibody titres of rams experimentally infected with H. ovis (11,31).…”

Section: Discussionmentioning

confidence: 99%

Trials with an Enzyme‐Linked Immunosorbent Assay (ELISA) for the Diagnosis of Subclinical Genital Infections in Rams Caused by Histophilus ovis and Actinobacillus seminis

Tekes¹,

Hajtós

1990

Journal of Veterinary Medicine, Series B

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“…The grow rates on tryptose agar were were compared with the extracellular, facultative anaerobic, gram-negative bacilli in the division Gracilicutes, particu-similar to those on blood agar. [19][20][21]) and the 2 field strains in group B strains were oxidase-positive, the A. actinomycetem-(numbers 22 and 23) were not identified to either genus comitans and A. seminis strains generally had weak or species level based on the descriptions of currently positive reactions, and some replicates gave negative classified organisms.…”

Section: Methodsmentioning

confidence: 99%

Cultural and Biochemical Characterization ofActinobacillusandActinobacillus-LikeSpecies from Ram Lambs with Epididymitis

et al. 1989

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Abstract. Cellular, colonial, cultural, and biochemical characteristics of 25 field strains of gram-negative pleomorphic bacilli from rams with epididymitis were compared with Actinobacillus actinomycetemcomitans American Type Culture Collection (ATCC) strain 29522 and Actinobacillus seminis ATCC strain 15768. Three field strains were identified as A. actinomycetemcomitans, 15 as A. seminis, and 2 as Haemophilus agni; however, 5 strains (3 in group A and 2 in group B) were not identified as species in the genera Actinobacillus, Haemophilus, or Pasteurella based on the taxonomic criteria in Bergey's manual of systematic bacteriology. The 5 Actinobacillus-like organisms in groups A and B were predominantly gram-negative coccobacilli and exhibited less pleomorphism than the 2 Actinobacillus species. The colonial morphologies of groups A and B were similar to the 2 Actinobacillus species but were smaller in diameter and had a pale yellow color. Groups A and B, like the actinobacilli, were facultative anaerobic and capnophilic, did not grow on MacConkey agar, and were catalasepositive and oxidase-positive. Group A reduced nitrate but group B did not. The A. seminis strains utilized ornithine, and group A utilized arginine; but group B did not utilize either omithine or arginine. All strains failed to utilize lysine or tryptophane. All strains produced acid but no gas from glucose, and the utilization of other carbohydrates varied markedly both between and within the 5 groups of bacteria. Based on carbohydrate utilization profiles, the 15 field strains of A. seminis represented 13 biotypes, but with a commercial semiquantitative enzyme system only 1 biotype was identified. All the A. actinomycetemcomitans, A. seminis, and group B strains produced moderate to high levels of alkaline phosphatase and acid phosphatase, but the group A strains were negative. Groups A and B were negative for beta-glucuronidase, but all the A. actinomycetemcomitans and A. seminis strains produced this enzyme.Actinobacillus seminis, Haemophilus agni, and Histophilus ovis were first reported as new bacterial species in 1960, 1958, and 1956, respectively. 2,11,19 The A. seminis isolate was from a ram with epididymitis, and the H. agni and H. ovis isolates were from sheep with other disease conditions. Subsequently, these bacterial species and a few other species in the genera Actinobacillus and Haemophilus, as well as other gram-negative pleomorphs that were frequently referred to as Actinobacillus-like or Haemophilus-like organisms, were isolated from either epididymal lesions or semen from rams with epididymitis. Various biochemical test methodologies were employed in these studies, and constituent properties of the organisms, such as the presence of cytochrome c, nitrate reductase, and the ability to utilize glucose, often have been reported as variable characteristics, resulting Received for publication April 11, 1989. in considerable confusion in the characterization and classification of these organisms. Several studies in the 198...

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EXAMINATION OF QUEENSLAND ISOLATES OF SALMONELLA PULLORUM FOR VARIATION OF ANTIGEN 12

McDougall¹

1967

Aust Veterinary J

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Epidemiology of Actinobacillus Seminis in a Flock of Border Leicester Sheep

Cited by 5 publications

References 0 publications

Trials with an Enzyme‐Linked Immunosorbent Assay (ELISA) for the Diagnosis of Subclinical Genital Infections in Rams Caused by Histophilus ovis and Actinobacillus seminis

Trials with an Enzyme‐Linked Immunosorbent Assay (ELISA) for the Diagnosis of Subclinical Genital Infections in Rams Caused by Histophilus ovis and Actinobacillus seminis

Cultural and Biochemical Characterization ofActinobacillusandActinobacillus-LikeSpecies from Ram Lambs with Epididymitis

EXAMINATION OF QUEENSLAND ISOLATES OF SALMONELLA PULLORUM FOR VARIATION OF ANTIGEN 12

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