Epidemiology, molecular characterization, and recombination analysis of chicken anemia virus in Guangdong province, China

Tan, Chen; Wang, Zhanxin; Lei, Xiaoya; Jiang, Lu; Zhang, Yan; Qin, Jian-Ping; Chen, Feng; Xie, Qingmei; Lin, Wencheng

doi:10.1007/s00705-020-04604-8

Cited by 21 publications

(22 citation statements)

References 36 publications

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“…As a DNA virus, the genomic recombination of CAV had a low efficiency. However, previous studies had shown evidence of genetic recombination events ( 20 , 21 , 32 ). Consistent with these reports, reliable recombination events were also detected in this study.…”

Section: Discussionmentioning

confidence: 93%

Genomic Characterization of Chicken Anemia Virus in Broilers in Shandong Province, China, 2020–2021

Liu

Li²,

Yin

et al. 2022

Front. Vet. Sci.

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Chicken infectious anemia (CIA), caused by chicken anemia virus (CAV), is an immunosuppressive disease characterized by growth retardation, aplastic anemia, lymphoid depletion, and immunodepression in young chickens. In this study, 33 CAV strains were isolated from broilers in Shandong Province during 2020–2021. Phylogenetic analysis of full-length genome sequences showed that most CAV strains isolated in this study were scattered across different branches, but mainly clustered in two genotypes, indicating a certain regional characteristic. Analysis of VP1 protein identified several amino acid substitutions which were relevant with the virulence and virus spread efficiency. Interestingly, four putative DNA recombination events were detected in the genomes of novel isolated CAV strains. In summary, this study demonstrated a genomic diversity of CAV in broilers isolated in Shandong Province during 2020–2021, and provided information for the further study of CAV molecular epidemiology and viral evolution.

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Section: Discussionmentioning

confidence: 93%

Genomic Characterization of Chicken Anemia Virus in Broilers in Shandong Province, China, 2020–2021

Liu

Li²,

Yin

et al. 2022

Front. Vet. Sci.

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“…These isolates were further divided into three branches, where HN-3, HN-5, HN-7, HN-10, and HN-11 were all on the same branch as the Japanese classical attenuated strain C369 and the Korean attenuated strain CIAV 89-69 [22]. Among them, HN-11 was on the same small branch as Guangdong isolate GD-1-12 [23]. The three strains HN-2, HN-4 and HN-6 were on the same branch as the former Henan HN1405 strain, whereas the HN-1, HN-8, HN-9 and HN-12 strains were on the same branch as the former Henan HN1504 strain, indicating that these strains might be the dominant strains in Henan province.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Isolated Chicken Infectious Anemia Viruses in Central China

Wang¹,

Feng²,

Liu³

et al. 2021

Preprint

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Background: Chicken infectious anemia (CIA) is an immunosuppressive disease induced by the chicken infectious anemia virus (CIAV) causing heavy economic losses once outbreak. This study conducted a systematic analysis of the epidemiology and pathology of CIA in Henan province, central China.Methods: A total of 437 clinical tissue samples and 120 poultry disease-related live attenuated vaccines were collected during 2017-2019; of which 45 were positive for CIAV nucleic acid, with a positive rate of 8.08%. Results: Our results showed that genome sequences similarity among a total of 12 CIAV isolates was high, and ranged from 97.1% to 99.3%, and their similarity to the vaccine strains Cux-1 and Del-Ros ranged from 97.8% to 98.6%. However, There were non-synonymous amino acid mutations in the locus of the major capsid proteins VP1, VP2 and VP3 among all isolates. The subsequent sequence analysis indicated that the isolates of HN-4 and HN-8 showed genetic recombination and follow up animal experiments revealed that HN-4 might be a high pathogenic strain. Conclusions: Our results reveal that both field infection and vaccine contamination promote epidemiology of CIAV in China. Some dominant epidemic viruses have undergone recombination and evolution. This study provides important information for CIAV prevention and control in poultry industry development.

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“…After its rst report in 1979 (Yuasa et al, 1979), CIAV has been detected worldwide and has caused huge economic losses (Fatoba et al,2019). Recently, CIAV infections have also frequently appeared in different chicken farms in China (Zhang et al, 2013;Fang et al, 2017;Tan et al, 2020). The pathogenesis of CIAV has been well understood that it mainly causes the atrophy of bone marrow, hematopoietic tissue, and lymphatic tissues (e.g., thymus) in young chickens (Adair et al,2000).…”

Section: Introductionmentioning

confidence: 99%

Genomic characteristics of a chicken infectious anemia virus strain isolated from contaminated chicken pox attenuated vaccine

Chen

Wang

et al. 2021

Preprint

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Chicken infectious anemia virus (CIAV) can be transmitted by contaminated live vaccines, and causes huge economic losses. This study evaluated the contamination status of CIAV in 24 batches of vaccines by recombinase-aided amplification assay (RAA), fluorescence quantitative PCR and dot blot assay, and then determined a contaminated avian pox attenuated vaccine. The whole genome of the CIAV contaminant was then sequenced and named as JS2020-PFV (Genbank accession number: MW234428, 2296bp). It showed 94.5–99.9% identities with reference strains, and shared the closest evolution relationship with AB1K which was isolated from a chicken farm in Turkey. All these suggested that the use of CIAV contaminated live vaccine may be the reason for its epidemic in poultry.

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Epidemiology, molecular characterization, and recombination analysis of chicken anemia virus in Guangdong province, China

Cited by 21 publications

References 36 publications

Genomic Characterization of Chicken Anemia Virus in Broilers in Shandong Province, China, 2020–2021

Genomic Characterization of Chicken Anemia Virus in Broilers in Shandong Province, China, 2020–2021

Molecular Characterization of Isolated Chicken Infectious Anemia Viruses in Central China

Genomic characteristics of a chicken infectious anemia virus strain isolated from contaminated chicken pox attenuated vaccine

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