Epidemiology and molecular characterization of a clone of Burkholderia cenocepacia responsible for nosocomial pulmonary tract infections in a French intensive care unit

Graindorge, Arnault; Menard, Aymeric; Neto, Manuelle; Bouvet, Claude; Miollan, Roger; Gaillard, Sandrine; Montclos, H. De; Laurent, François; Cournoyer, Benoît

doi:10.1016/j.diagmicrobio.2009.06.008

Cited by 23 publications

(18 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to their recognition as CF pathogens, both B. multivorans and B. cenocepacia have also been responsible for outbreaks amongst hospitalized non-CF patients, often being associated with poor patient outcomes (Woods et al, 2004;Mann et al, 2010;Graindorge et al, 2010;Hanulik et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Growth on mannitol-rich media elicits a genome-wide transcriptional response in Burkholderia multivorans that impacts on multiple virulence traits in an exopolysaccharide-independent manner

et al. 2014

View full text Add to dashboard Cite

In common with other members of the Burkholderia cepacia complex (BCC), Burkholderia multivorans is capable of producing exopolysaccharide (EPS) when grown on certain mannitolrich media. The significance of the resulting mucoid phenotype and the genome-wide response to mannitol has never been characterized despite its clinical relevance following the approval of a dried-powder preparation of mannitol as an inhaled osmolyte therapy for cystic fibrosis (CF) patients. In the present study we defined the transcriptional response of B. multivorans ATCC 17616, a model genome-sequenced strain of environmental origin, to growth on mannitol-rich yeast extract media (MYEM). EPS-dependent and -independent impact of MYEM on virulenceassociated traits was assessed in both strain ATCC 17616 and the CF isolate B. multivorans C1576. Our studies revealed a significant transcriptional response to MYEM encompassing approximately 23 % of predicted genes within the genome. Strikingly, this transcriptional response identified that EPS induction occurs in ATCC 17616 without the upregulation of the bce-I and bce-II EPS gene clusters, despite their pivotal role in EPS biosynthesis. Of approximately 20 differentially expressed putative virulence factors, 16 exhibited upregulation including flagella, ornibactin, oxidative stress proteins and phospholipases. MYEM-grown B. multivorans also exhibited enhanced motility, biofilm formation and epithelial cell invasion. In contrast to these potential virulence enhancements, MYEM-grown B. multivorans C1576 showed attenuated virulence in the Galleria mellonella infection model. All of the observed phenotypic responses occurred independently of EPS production, highlighting the profound impact that mannitol-based growth has on the physiology and virulence of B. multivorans.

show abstract

Section: Introductionmentioning

confidence: 99%

Growth on mannitol-rich media elicits a genome-wide transcriptional response in Burkholderia multivorans that impacts on multiple virulence traits in an exopolysaccharide-independent manner

et al. 2014

View full text Add to dashboard Cite

show abstract

“…This strain was thus used as a reference throughout this study. This strain belongs to the ET12 clonal complex and was found to be a close relative of the B&B clone (Graindorge et al, 2010). IS DNA hybridizations of genomic DNA blots of strain bpoe717 clearly indicated a B&B IS content similar to that of the J2315 genome, with at least 12 IS3 elements, at least eight IS5 elements and 10 IS110 elements.…”

Section: Discussionmentioning

confidence: 99%

“…This strain was isolated in 2004 from a mechanically ventilated patient hospitalized in a French intensive-care unit who died of respiratory failure 3 days after the diagnosis of a nosocomial pulmonary infection (Graindorge et al, 2010). Its genomic DNA was extracted as described by Pitcher et al (1989) and partially digested by Sau3AI (Fermentas).…”

Section: Methodsmentioning

confidence: 99%

“…Other North American epidemic clones have been described and named PDHC and Midwest (Coenye et al, 2004;LiPuma et al, 1988). The B&B clone is a French epidemic clone that was described recently (Graindorge et al, 2010). This clone and its related strains are part of the ST32 clonal complex, which has been found to be involved in nosocomial and CF pulmonary tract infections and has disseminated among several occidental countries.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Insertion sequence evolutionary patterns highlight convergent genetic inactivations and recent genomic island acquisitions among epidemic Burkholderia cenocepacia

Graindorge

Menard

Monnez

et al. 2012

Journal of Medical Microbiology

Self Cite

View full text Add to dashboard Cite

The Burkholderia cenocepacia B&B clone was found previously to be responsible for an epidemic outbreak within an intensive care unit in France. This clone belongs to the ST32 clonal complex, which is one of the most prevalent among French cystic fibrosis patients and is known to be related to the highly virulent ET12 clonal complex. Genomic repartition biases of insertion sequences (ISs) were investigated to improve our understanding of the evolutionary events leading to B. cenocepacia diversification and the emergence of such epidemic lineages. IS were used for tracking convergent genetic inactivations and recent DNA acquisitions. B. cenocepacia IS families and subgroups were compared in terms of genetic diversity and genomic architecture using fully sequenced genomes, PCR screening and DNA blot analysis. These analyses revealed several features shared by the B&B and ET12 epidemic clones. IS elements showed a frequent localization on genomic islands (GI) and indicated convergent evolution towards inactivation of certain loci. The IS407 subgroup of the IS3 family was identified as a good indicator of recently acquired GIs in clone ET12. Several IS407 elements showed strain-specific or clonal complex-specific localizations. IS407 DNA probing of a DNA library built from the B. cenocepacia B&B epidemic clone led to the identification of a recently acquired IS407-tagged GI likely to be conjugative and integrative. The B&B clone showed significant differences in its IS architecture from that of ST32 strains isolated from Czech cystic fibrosis patients.

show abstract

“…It has been proposed that the large divergence in 16S rRNA sequence similarity between the different Burkholderia lineages is indicative of different species clusters, with one lineage made up of saprophytic species and another made up of the Bcc, pathogens, and opportunistic pathogens of plants, humans, and animals (54). Several research groups have successfully applied Bcc-specific recA-based PCR test as a diagnostic tool for the identification of B. cepacia complex isolates in clinical (42,55) and environmental (56)(57)(58) settings. Specific recA primers have also been used to determine relatedness of potential biocontrol and environmental isolates to Bcc species (59)(60)(61).…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Burkholderia rinojensis sp. nov., a Non-Burkholderia cepacia Complex Soil Bacterium with Insecticidal and Miticidal Activities

Córdova-Kreylos

Fernandez

Koivunen

et al. 2013

Appl Environ Microbiol

View full text Add to dashboard Cite

Isolate A396, a bacterium isolated from a Japanese soil sample demonstrated strong insecticidal and miticidal activities in laboratory bioassays. The isolate was characterized through biochemical methods, fatty acid methyl ester (FAME) analysis, sequencing of 16S rRNA, multilocus sequence typing and analysis, and DNA-DNA hybridization. FAME analysis matched A396 to Burkholderia cenocepacia, but this result was not confirmed by 16S rRNA or DNA-DNA hybridization. 16S rRNA sequencing indicated closest matches with B. glumae and B. plantarii. DNA-DNA hybridization experiments with B. plantarii, B. glumae, B. multivorans, and B. cenocepacia confirmed the low genetic similarity (11.5 to 37.4%) with known members of the genus. PCR-based screening showed that A396 lacks markers associated with members of the B. cepacia complex. Bioassay results indicated two mechanisms of action: through ingestion and contact. The isolate effectively controlled beet armyworms (Spodoptera exigua; BAW) and two-spotted spider mites (Tetranychus urticae; TSSM). In diet overlay bioassays with BAW, 1% to 4% (vol/vol) dilution of the whole-cell broth caused 97% to 100% mortality 4 days postexposure, and leaf disc treatment bioassays attained 75% ؎ 22% mortality 3 days postexposure. Contact bioassays led to 50% larval mortality, as well as discoloration, stunting, and failure to molt. TSSM mortality reached 93% in treated leaf discs. Activity was maintained in cell-free supernatants and after heat treatment (60°C for 2 h), indicating that a secondary metabolite or excreted thermostable enzyme might be responsible for the activity. Based on these results, we describe the novel species Burkholderia rinojensis, a good candidate for the development of a biocontrol product against insect and mite pests.

show abstract

Epidemiology and molecular characterization of a clone of Burkholderia cenocepacia responsible for nosocomial pulmonary tract infections in a French intensive care unit

Cited by 23 publications

References 58 publications

Growth on mannitol-rich media elicits a genome-wide transcriptional response in Burkholderia multivorans that impacts on multiple virulence traits in an exopolysaccharide-independent manner

Growth on mannitol-rich media elicits a genome-wide transcriptional response in Burkholderia multivorans that impacts on multiple virulence traits in an exopolysaccharide-independent manner

Insertion sequence evolutionary patterns highlight convergent genetic inactivations and recent genomic island acquisitions among epidemic Burkholderia cenocepacia

Isolation and Characterization of Burkholderia rinojensis sp. nov., a Non-Burkholderia cepacia Complex Soil Bacterium with Insecticidal and Miticidal Activities

Contact Info

Product

Resources

About