Epidemiology and genotypic characterisation of dissemination patterns of uropathogenicEscherichia coliin a community

Abstract

To characterise the dissemination patterns of uropathogenic Escherichia coli (UPEC) in a community, we conducted a study utilising molecular and fundamental descriptive epidemiology. The subjects, consisted of women having community-acquired acute urinary tract infection (UTI), were enrolled in the study from 2011 to 2012. UPEC isolates were subjected to antibacterial-susceptibility testing, O serogrouping, phylotyping, multilocus-sequence typing with phylogenetic-tree analysis and pulsed-field-gel electrophoresis (PFGE). From the 209 unique positive urinary samples 166 UPEC were isolated, of which 129 were fully susceptible to the tested antibiotics. Of the 53 sequence types (STs), the four most prevalent STs (ST95, ST131, ST73 and ST357) accounted for 60% of all UPEC strains. Antimicrobial resistance was less frequently observed for ST95 and ST73 than for the others. A majority of rare STs and a few common STs constituted the diversity pattern within the population structure, which was composed of the two phylogenetically distinct clades. Eleven genetically closely related groups were determined by PFGE, which accounted for 42 of the 166 UPEC isolates, without overt geo-temporal clustering. Our results indicate that a few major lineages of UPEC, selected by unidentified factors, are disseminated in this community and contribute to a large fraction of acute UTIs.

Global Evolution of Pathogenic Bacteria With Extensive Use of Fluoroquinolone Agents

“…However, the distinction in virulence between hospital-associated and community-acquired strains is not as unequivocal as with MRSA and MDR K. pneumoniae because in contrast to these agents CA-E. coli strains, as primarily urogenital pathogens, are often exposed to fluoroquinolones and other antibiotics impacting the fitness of the isolates in a clonedependent fashion. This is the reason why in contrast to MRSA and MDR K. pneumonaiae the fluoroquinolone-selected clone of MDR E. coli, ST131, may feature prominently in communityacquired infections (Blanco et al, 2013;Doi et al, 2013;Nicolas-Chanoine et al, 2014;Chong et al, 2018;Jamborova et al, 2018;Matsukawa et al, 2019).…”

<p>Whole-genome sequence analysis of multidrug-resistant uropathogenic strains of <em>Escherichia coli</em> from Mexico</p>

“…In another study carried out in UPEC strains (n=167), the most frequent ST identified was ST131 (n=20), mainly corresponding to phylogenetic group B2, which also includes ST ST-1193, ST-14, ST-10 and ST-69, found in the present study (Table 1). 47 In this study, group O25:H4-ST-131, belonging to phylogenetic group B2 of E. coli was the most prevalent group in the patients studied. Therefore, it is important to establish epidemiological monitoring programs in the region, focused specifically on controlling bacterial resistance to antimicrobials.…”

Anti-Bacterial Effects of Essential Oils against Uropathogenic Bacteria

“…Therefore, we tested for synergistic activity using checkerboard assays against antibiotic-sensitive E. coli strains as the major causative agent for uncomplicated cystitis. Strains were selected according to their sequence types to represent four of the most prevalent sequence types of cystitis causing E. coli —131 ( E. coli CHD94), 95 ( E. coli UTI89), 73 ( E. coli CHD3), and 69 ( E. coli CHD22) [ 14 ]. The combination of thyme and tea tree resulted in an additive 8-fold reduction of the MIC and MBC values of both essential oils compared to the substances alone ( Table 2 ).…”