Epidemiologically related and unrelated strains of Pseudomonas aeruginosa serotype O12 cannot be distinguished by phenotypic and genotypic typing

Mifsud, Amparo; Watine, Joseph; Picard, Bertrand; Charet, Jean-Christophe; Solignac-Bourrel, C; Pitt, T. L.

doi:10.1016/s0195-6701(97)90116-x

Cited by 20 publications

(14 citation statements)

References 19 publications

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“…The inadequacy of antibiotype to confirm a clonal relation among multiresistant isolates has been reported (6,10). However, we found antibiotyping useful since all the multiresistant isolates of this study were included in the same genotype.…”

Section: Discussionmentioning

confidence: 61%

“…The combination of the two methods was also insufficient to establish a distinction among isolates from different genotypes. In fact, failure to obtain good quality data using phenotypic methods has been reported previously, also with respect to isolates from cystic fibrosis patients (1,5,6,9,10,20,21).…”

Section: Discussionmentioning

confidence: 85%

“…Although susceptibility tests provide profiles that are used in infection control, in most cases these data cannot confirm clusters of nosocomial cross-infection or distinguish unrelated infections (5,(7)(8)(9), especially when multiresistant strains are analyzed (6,10). However, quantitative analysis of antibiograms by comparing disk zone sizes has been considered useful for nosocomial infection control in a few situations (11).…”

Section: Introductionmentioning

confidence: 99%

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Typing of Pseudomonas aeruginosa from hospitalized patients: a comparison of susceptibility and biochemical profiles with genotype

Freitas

Barth

2004

Braz J Med Biol Res

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Typing techniques are essential for understanding hospital epidemiology, permitting the elucidation of the source of infection and routes of bacterial transmission. Although DNA-based techniques are the "gold standard" for the epidemiological study of Pseudomonas aeruginosa, antibiotic profiles and biochemical results are used because they are easy to perform and to interpret and relatively inexpensive. Antibiotypes (susceptibility profiles) and biotypes (biochemical profiles) were compared to genotypes established by DNA restriction enzyme analysis in 81 clinical isolates of P. aeruginosa from three hospitals in Porto Alegre, Brazil. The epidemiological relationship among patients was also evaluated. Susceptibility and restriction profiles were discrepant in more than 50% of the cases, and many antibiotypes were observed among isolates from the same genotype. Furthermore, susceptibility profiles did not allow the distinction of isolates from unrelated genotypes. Since a large number of isolates (63%) yielded the same biochemical results, only 10 biotypes were detected, showing that this typing method has a low discriminatory power. On the other hand, DNA restriction enzyme typing allowed us to establish 71 distinct types. Epidemiological data about the relation among P. aeruginosa isolates were not conclusive. The results of the present study indicate that the only method that can establish a clonal relation is DNA restriction enzyme typing, whereas the other methods may cause misleading interpretations and are inadequate to guide proper infection control measures.

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Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

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Typing of Pseudomonas aeruginosa from hospitalized patients: a comparison of susceptibility and biochemical profiles with genotype

Freitas

Barth

2004

Braz J Med Biol Res

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“…Fifteen years ago, O12 was the major serotype of P. aeruginosa strains hosting the PSE-1 ␤-lactamase, at least in Europe (26). It may be that serotype O12 strains, which form a tight cluster in terms of DNA relatedness (21,25), are particularly adept at acquiring or hosting the integrons that encode the IMP, VIM, and PSE enzymes, but this hypothesis is speculative.…”

Section: Description Of Outbreakmentioning

confidence: 99%

Outbreak of Carbapenem-Resistant Pseudomonas aeruginosa Producing VIM-8, a Novel Metallo-β-Lactamase, in a Tertiary Care Center in Cali, Colombia

Crespo

Woodford²,

Sinclair³

et al. 2004

J Clin Microbiol

143

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The prevalence of imipenem resistance among Pseudomonas aeruginosa isolates at a 195-bed tertiary care medical center in Cali, Colombia, rose from 2% in 1996 to 28% in 1997 and to over 40% in 2003. Many isolates showed high-level multiresistance, and phenotypic characterization suggested the spread of a predominant strain with minor variants. Sixty-six resistant isolates collected between February 1999 and July 2003 from hospitalized patients (n ‫؍‬ 54) and environmental samples (n ‫؍‬ 12) were subjected to a fuller analysis. Genetic fingerprints were compared by pulsed-field gel electrophoresis (PFGE) of SpeI-digested genomic DNA, and bla IMP and bla VIM genes were sought by PCR. PFGE and serotyping indicated that 52 of the 66 isolates belonged to a single strain, with 82% similarity; the PFGE pattern for this organism was designated pattern A. Two further pairs of isolates represented single strains; the remaining nine isolates were unique, and in the case of one isolate, no satisfactory PFGE profile could be obtained. The pattern A isolates were mostly of serotype O12 and were highly resistant to imipenem (MICs, 32 to >256 g/ml), with this resistance decreased eightfold or more in the presence of EDTA. They yielded amplicons with bla VIM -specific primers, and sequencing of DNA from a representative isolate revealed bla VIM-8 , a novel allele with three polymorphisms compared with the sequence of bla VIM-2 . Two of these nucleotide changes were silent, but the third determined a Thr139Ala substitution. Only 4 of 13 resistant isolates (2 clinical isolates and 2 environmental isolates) assigned to other PFGE types carried bla VIM alleles, whereas the others were less multiresistant and mostly had lower levels of imipenem resistance (MICs, <32 g/ml) which was not significantly reduced by EDTA. No bla IMP alleles were detected. During 2003, when the environmental study was undertaken, serotype O12 isolates with bla VIM were recovered from sinks and stethoscopes in the most-affected units, although not from the hands of staff; the problem declined once these reservoirs were disinfected and hygienic precautions were reinforced.

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“…The isolates 22843 and 27135 (VIM-2) were classified into ST111, a founder of the clonal complex CC4, corresponding to the major European P. aeruginosa clone P12 (9). P12 members express various resistance mechanisms, including MBLs (2,7,9,10,12). Interestingly, the "GreekSwedish" isolate with In59 represented ST229, which also belongs to CC4 (7).…”

mentioning

confidence: 99%

First Organisms with Acquired Metallo-β-Lactamases (IMP-13, IMP-22, and VIM-2) Reported in Austria

Duljasz

Gniadkowski

Sitter

et al. 2009

Antimicrob Agents Chemother

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Epidemiologically related and unrelated strains of Pseudomonas aeruginosa serotype O12 cannot be distinguished by phenotypic and genotypic typing

Cited by 20 publications

References 19 publications

Typing of Pseudomonas aeruginosa from hospitalized patients: a comparison of susceptibility and biochemical profiles with genotype

Typing of Pseudomonas aeruginosa from hospitalized patients: a comparison of susceptibility and biochemical profiles with genotype

Outbreak of Carbapenem-Resistant Pseudomonas aeruginosa Producing VIM-8, a Novel Metallo-β-Lactamase, in a Tertiary Care Center in Cali, Colombia

First Organisms with Acquired Metallo-β-Lactamases (IMP-13, IMP-22, and VIM-2) Reported in Austria

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