Epidemiological Study of &lt;I&gt;Fusarium&lt;/I&gt; Species Causing Invasive and Superficial Fusariosis in Japan

Muraosa, Yasunori; Oguchi, Misato; Yahiro, Maki; Watanabe, Akira; Yaguchi, Takashi; Kamei, Katsuhiko

doi:10.3314/mmj.16-00024

Cited by 27 publications

(12 citation statements)

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“…19,31 FSSC has been further divided into several phylogenetic groups by phylogenetic tree analysis. Hassan et al reported that FSSC3 + 4 (also called Fusarium falciforme ) was predominantly isolated from keratitis, 6 whereas Muraosa et al 32 reported that FSSC3 + 4 was the most frequent isolate collected from patients with superficial fusariosis in humans, followed by FSSC5. In our present study, 10 keratitis-derived FSSC isolates were distributed among the FSSC5 (n = 5), FSSC3 + 4 (n = 4), and FSSC9 (n = 1) complexes (Table 1).…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Luliconazole Against Broad-Range Filamentous Fungi Including Fusarium solani Species Complex Causing Fungal Keratitis

Todokoro

Suzuki

Tamura

et al. 2018

Cornea

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Purpose:Fungal keratitis can be difficult to medically treat. Topical antifungals are usually applied empirically as the initial option in treating fungal keratitis. Natamycin (NAT) and/or voriconazole (VRCZ) have been widely used in the treatment of fungal keratitis. However, Fusarium solani species complex (FSSC), which are the dominant species of fungal keratitis, are resistant to VRCZ. This study investigated in vitro efficacy of luliconazole (LLCZ), a new imidazole antifungal, against FSSC and other filamentous fungi.Methods:A total of 18 Fusarium isolates and 7 others were grown on potato dextrose agar at 30 and 37°C. For Fusarium, species identification and phylogenetic tree analysis were performed based on elongation factor-1α (EF-1α) DNA sequencing. The broth microdilution method was used for antifungal susceptibility testing of 11 antifungal drugs including LLCZ.Results:The 18 identified Fusarium isolates belonged to FSSC (n = 13), Fusarium oxysporum species complex (FOSC; n = 2), Fusarium chlamydosporum species complex (FCSC; n = 1), Fusarium incarnatum-equiseti species complex (FIESC; n = 1), and Fusarium fujikuroi species complex (FFSC; n = 1). We further divided 13 FSSC isolates into 3 clades, FSSC5 (n = 8), FSSC3 + 4 (n = 4), and FSSC9-a (n = 1), with 8 FSSC strains growing at 37°C. LLCZ showed lowest minimum inhibitory concentrations (MICs) against all tested filamentous fungi, with a MIC90 against the Fusarium species of 0.06 μg/mL, whereas MIC90 for NAT and VRCZ were 4 and 8 μg/mL, respectively.Conclusions:LLCZ has the strongest in vitro antifungal activity among all drugs used against broad-range filamentous fungi including FSSC. LLCZ may potentially be a new medical treatment option for fungal keratitis.

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Section: Discussionmentioning

confidence: 99%

Efficacy of Luliconazole Against Broad-Range Filamentous Fungi Including Fusarium solani Species Complex Causing Fungal Keratitis

Todokoro

Suzuki

Tamura

et al. 2018

Cornea

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“…In Singapore, an outbreak of contact lens associated keratitis caused by FSSC species occurred in 2005 involving 66 patients (Jureen et al, 2008). An epidemiological study in Japan, from 1998 to 2015, found that FSSC species accounted for 72.6% of all fusariosis cases, of which 77.8% were invasive fusariosis (IF) (Muraosa et al, 2017). An analysis of a collection of environmental Fusarium isolates from the Malaysian highlands found that 66.1% of 1,449 isolates belonged to the FSSC (Manshor et al, 2012), highlighting their predominance in Malaysia.…”

Section: Introductionmentioning

confidence: 99%

A 23 bp cyp51A Promoter Deletion Associated With Voriconazole Resistance in Clinical and Environmental Isolates of Neocosmospora keratoplastica

et al. 2020

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In the fungal pathogen Aspergillus fumigatus, resistance to azole antifungals is often linked to mutations in CYP51A, a gene that encodes the azole antifungal drug target lanosterol 14α-demethylase. The aim of this study was to investigate whether similar changes could be associated with azole resistance in a Malaysian Fusarium solani species complex (FSSC) isolate collection. Most (11 of 15) clinical FSSC isolates were Neocosmospora keratoplastica and the majority (6 of 10) of environmental isolates were Neocosmospora suttoniana strains. All 25 FSSC isolates had high minimum inhibitory concentrations (MICs) for itraconazole and posaconazole, low MICs for amphotericin B, and various (1 to >32 mg/l) voriconazole susceptibilities. There was a tight association between a 23 bp CYP51A promoter deletion and high (>32 mg/l) voriconazole MICs; of 19 FSSC strains sequenced, nine isolates had voriconazole MICs > 32 mg/l, and they all contained the 23 bp CYP51A promoter deletion, although it was absent in the ten remaining isolates with low (≤12 mg/l) voriconazole MICs. Surprisingly, this association between voriconazole resistance and the 23 bp CYP51A promoter deletion held true across species boundaries. It was randomly distributed within and across species boundaries and both types of FSSC isolates were found among environmental and clinical isolates. Three randomly selected N. keratoplastica isolates with low (≤8 mg/l) voriconazole MICs had significantly lower (1.3-7.5 times) CYP51A mRNA expression levels than three randomly selected N. keratoplastica isolates with high (>32 mg/l) voriconazole MICs. CYP51A expression levels, however, were equally strongly induced (∼6,500-fold) by voriconazole in two representative strains reaching levels, after 80 min of induction, that were comparable to those of CYP51B. Our results suggest that FSSC isolates with high voriconazole MICs have a 23 bp CYP51A promoter deletion that provides a potentially useful marker for voriconazole resistance in FSSC isolates. Early detection of possible voriconazole resistance is critical for choosing the correct treatment option for patients with invasive fusariosis.

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“…Notes — Neocosmospora tonkinensis , previously known as FSSC 9, is known to include human pathogens, mostly isolated from corneal specimens (O’Donnell et al 2008, Muraosa et al 2017), as well as from animal infections (O’Donnell et al 2008, 2016). Short et al (2011) reported also the isolation of this species from water drains in the USA.…”

Section: Resultsmentioning

confidence: 99%

Removing chaos from confusion: assigning names to common human and animal pathogens in Neocosmospora

Sandoval-Denis

Crous

2018

persoonia

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The genus Neocosmospora encompasses highly prevalent and aggressive human and animal fungal pathogens. Here we assign formal descriptions and Latin binomials to some of the most clinically relevant phylogenetic species of the genus. Three new species, named Neocosmospora catenata , N. gamsii and N. suttoniana (previously assigned to the informal names ‘ Fusarium ’ solani species complex (FSSC) lineages, FSSC 43, FSSC 7 and FSSC 20, respectively) are described on the basis of multilocus phylogenetic analyses (using EF-1α , ITS, LSU and RPB2 loci) and morphological characters. Lineage FSSC 9 is conspecific with the ex-type strain of Cylindrocarpon tonkinense , thus the new combination Neocosmospora tonkinensis is proposed. In addition, and based on the latest taxonomy for this generic complex, new combinations are introduced for four medically important taxa : Neocosmospora keratoplastica , N. lichenicola , N. metavorans and N. petroliphila . The most significant distinctive features for all the clinically relevant species treated here are compared and illustrated.

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Epidemiological Study of <I>Fusarium</I> Species Causing Invasive and Superficial Fusariosis in Japan

Cited by 27 publications

References 50 publications

Efficacy of Luliconazole Against Broad-Range Filamentous Fungi Including Fusarium solani Species Complex Causing Fungal Keratitis

Efficacy of Luliconazole Against Broad-Range Filamentous Fungi Including Fusarium solani Species Complex Causing Fungal Keratitis

A 23 bp cyp51A Promoter Deletion Associated With Voriconazole Resistance in Clinical and Environmental Isolates of Neocosmospora keratoplastica

Removing chaos from confusion: assigning names to common human and animal pathogens in Neocosmospora

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