Epidemiological Study of AGT Gene Polymorphism among Chinese Subjects with Primary Hypertension

Abstract

Background: Polymorphisms in the promoter region of the angiotensinogen (AGT) gene may affect AGT transcription and thus blood pressure. We determined the frequency of the AGT A-20C polymorphism in Chinese patients with primary hypertension. Using a molecular epidemiology approach, we also determined the relationship between primary hypertension and environmental-AGT A-20C polymorphism interactions.Methods: 912 subjects diagnosed with primary hypertension were analyzed from Shenyang Chinese Populations and their samples were genotyped using A-20C polymorphism within angiotensinogen (AGT) gene by estriction ragment ength Polymorphism methods (PCR-RFLP)Results: PCR and restriction fragment length polymorphism techniques were used to determine the distribution frequency of AGT A-20C alleles. Frequencies of 84.7% and 15.3% were observed for the A and C alleles, respectively. The polymorphism was in Hardy-Weinberg equilibrium according to a χ 2 test (χ 2 =0.58, P>0.05). Linear regression of AGT genotype and blood pressure revealed that systolic blood pressure was significantly higher for AC and CC genotypes compared to the wild-type AA genotype in females (P<0.05). Conclusion:This is the first report on the frequency of AGT A-20C polymorphism in a Chinese population. Compared to the AA genotype, the effects of genotypes AC and CC on blood pressure were mainly manifest as significantly higher systolic blood pressure in females.

AGT A-20C (rs5050) gene polymorphism and ulcer occurrence in patients treated with low-dose aspirin: a case-control study / Polimorfismul AGT A-20C și ulcerele gastro-duodenale la pacienții sub tratament cu aspirină în doze antiagregante: studiu caz-control

“…The AGT A-20C gene polymorphism was investigated by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) technique using the primers (from Eurogentec) and restriction enzyme (FastDigest EcoO109I from Thermoscientific) as previously described [9]. The PCR conditions were similar to those described by Zhang et al [13].…”

The angiotensinogen gene polymorphism, lifestyle factors, associated diseases and gastric areas of inflammatory and preneoplastic lesions in a Romanian sample of patients

“…DNA was extracted from venous blood samples of each subject collected in EDTA (Ethylenediaminetetraacetic acid)-containing tubes, using a commercially available kit (Quick-gDNAMini-Prep, ZymoResearch, USA). The genotypes of the investigated polymorphism of AGT gene, namely c.-58 A>C gene polymorphism, were established by using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique and specific restriction enzyme as previously described (14,15,16). The inclusion criteria for both groups were: adult subjects without malignancies, with complete medical and clinical data, referred for endoscopic evaluation, with at least four routine biopsies (two from the antrum and two from the corpus) who signed the informed consent.…”