Epidemiological Investigation of Hospital Transmission of Corynebacterium striatum Infection by Core Genome Multilocus Sequence Typing Approach

Kang, Yutong; Chen, Shenglin; Zheng, Beijia; Du, Xin; Li, Zhenpeng; Tan, Zhizhou; Zhou, Haijian; Huang, Jia; Tian, Leihao; Zhong, Jiaxin; Ma, Xueli; Li, Fang; Yao, Jianquan; Wang, Yu; Zheng, Meiqin; Li, Zhenjun

doi:10.1128/spectrum.01490-22

Cited by 3 publications

(3 citation statements)

References 65 publications

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“…In epidemiological studies of infectious diseases, strain typing contributes to the understanding of bacterial transmission routes [ 32 , 33 ]. In this study, MRBP isolates were effectively differentiated by identifying SNP differences through short-read sequencing.…”

Section: Discussionmentioning

confidence: 99%

Whole-genome comparison of two same-genotype macrolide-resistant Bordetella pertussis isolates collected in Japan

Koide,

Uchitani,

Yamaguchi

et al. 2024

PLoS ONE

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The emergence of macrolide-resistant Bordetella pertussis (MRBP) is a significant problem because it reduces treatment options for pertussis and exacerbates the severity and spread of the disease. MRBP has been widely prevalent in mainland China since the 2010s and has been sporadically detected in other Asian countries. In Japan, two MRBP clinical strains were first isolated in Tokyo and Osaka between June and July 2018. The isolates BP616 in Osaka and BP625 in Tokyo harbored the same virulence-associated allelic genes (including ptxP1, ptxA1, prn1, fim3A, and fhaB3) and MT195 genotype and exhibited similar antimicrobial susceptibility profiles. However, despite their simultaneous occurrence, a distinguishable epidemiological link between these isolates could not be established. To gain further insight into the genetic relationship between these isolates in this study, we performed whole-genome analyses. Phylogenetic analysis based on genome-wide single-nucleotide polymorphisms revealed that the isolates belonged to one of the three clades of Chinese MRBP isolates, but there were 11 single-nucleotide polymorphism differences between BP616 and BP625. Genome structure analysis revealed two large inversions (202 and 523 kbp) and one small transposition (3.8 kbp) between the genomes. These findings indicate that the two Japanese MRBP isolates are closely related to Chinese MRBP isolates but are genomically distinct, suggesting that they were introduced into Japan from mainland China through different transmission routes.

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Section: Discussionmentioning

confidence: 99%

Whole-genome comparison of two same-genotype macrolide-resistant Bordetella pertussis isolates collected in Japan

Koide,

Uchitani,

Yamaguchi

et al. 2024

PLoS ONE

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“…It can be challenging to distinguish between an actual infection and colonization from patient specimens. To differentiate, it is crucial to repeatedly identify the organism, isolate it from a sterile site, and maintain a high level of clinical suspicion in the appropriate host, especially in cases involving immunocompromised individuals or medical devices [ 7 ].…”

Section: Discussionmentioning

confidence: 99%

Infective Endocarditis Caused by Corynebacterium striatum: Navigating Challenges and Treatment Strategies in an Emerging Threat

Gaifer,

Samman,

Albluwi

2023

Cureus

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Corynebacterium striatum is a type of bacteria normally found in the environment and is considered a benign microbe on the human body surface. However, it can induce severe medical conditions, including bacteremia, infective endocarditis, osteomyelitis, and infections, in other organs. This case study focuses on a 56-year-old male patient with multiple comorbidities who presented with an ischemic stroke. Several days after the insertion of a right internal jugular line, the patient developed fever and tachycardia. Blood cultures revealed the presence of Corynebacterium striatum , a Gram-positive bacilli. Transesophageal echocardiography confirmed the diagnosis of complicated infective endocarditis (IE) with mitral valve vegetation and moderate mitral regurgitation. Prompt treatment with appropriate antibiotics, including linezolid and later vancomycin, led to the patient's improvement and eventual discharge in good condition. This case highlights the importance of early recognition, aggressive management, and accurate diagnosis in cases of IE caused by Corynebacterium striatum . Proper antibiotic selection is crucial, considering the emerging antibiotic resistance patterns associated with this pathogen. By addressing these aspects, patient outcomes can be improved, and potential complications such as IE can be prevented.

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“…The training file was downloaded from GitHub ( https://github.com/B-UMMI/chewBBACA/blob/master/CHEWBBACA/prodigal_training_files/Enterococcus_faecium.trn ). Allele calling from the core genome was performed using all genomes as previously described ( 40 ). A minimum spanning tree was constructed using PHYLOViZ Online ( https://online.phyloviz.net/index ).…”

Section: Methodsmentioning

confidence: 99%

Genomic analysis of inter-hospital transmission of vancomycin-resistant Enterococcus faecium sequence type 80 isolated during an outbreak in Hiroshima, Japan

Segawa,

Masuda,

Hisatsune

et al. 2024

Antimicrob Agents Chemother

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Outbreaks caused by vancomycin-resistant enterococci that transcend jurisdictional boundaries are occurring worldwide. This study focused on a vancomycin-resistant enterococcus outbreak that occurred between 2018 and 2021 across two cities in Hiroshima, Japan. The study involved genetic and phylogenetic analyses using whole-genome sequencing of 103 isolates of vancomycin-resistant enterococci to identify the source and transmission routes of the outbreak. Phylogenetic analysis was performed using core genome multilocus sequence typing and core single-nucleotide polymorphisms; infection routes between hospitals were inferred using BadTrIP. The outbreak was caused by Enterococcus faecium sequence type (ST) 80 carrying the vanA plasmid, which was derived from strain A10290 isolated in India. Of the 103 isolates, 93 were E. faecium ST80 transmitted across hospitals. The circular vanA plasmid of the Hiroshima isolates was similar to the vanA plasmid of strain A10290 and transferred from E. faecium ST80 to other STs of E. faecium and other Enterococcus species by conjugation. The inferred transmission routes across hospitals suggest the existence of a central hospital serving as a hub, propagating vancomycin-resistant enterococci to multiple hospitals. Our study highlights the importance of early intervention at the key central hospital to prevent the spread of the infection to small medical facilities, such as nursing homes, with limited medical resources and a high number of vulnerable individuals.

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Epidemiological Investigation of Hospital Transmission of Corynebacterium striatum Infection by Core Genome Multilocus Sequence Typing Approach

Abstract: Recently, WGS of many human and animal pathogens has been successfully used to investigate microbial outbreaks. The cgMLST schema are powerful genotyping tools that can be used to investigate potential epidemics and provide classification of the strains precise and reliable.

Cited by 3 publications

References 65 publications

Whole-genome comparison of two same-genotype macrolide-resistant Bordetella pertussis isolates collected in Japan

Whole-genome comparison of two same-genotype macrolide-resistant Bordetella pertussis isolates collected in Japan

Infective Endocarditis Caused by Corynebacterium striatum: Navigating Challenges and Treatment Strategies in an Emerging Threat

Genomic analysis of inter-hospital transmission of vancomycin-resistant Enterococcus faecium sequence type 80 isolated during an outbreak in Hiroshima, Japan

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