Epidemiological investigation and phylogenetic analysis of caprine parainfluenza virus type 3 in sheep of China

Li, Mao; Yang, Leilei; Li, Wenliang; Liang, Panhong; Zhang, Shaohua; Li, Jizong; Sun, Min; Zhang, Wenwen; Wang, Liqun; Zhong, Cheng; Liu, Maojun; Ji, Jiang; Xue-peng, Cai; Luo, Xuenong

doi:10.1111/tbed.13149

Cited by 17 publications

(13 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since its discovery, CPIV3 has caught the attention of researchers in goat and sheep respiratory diseases due to its high prevalence and pathogenicity. Our previous report showed that CPIV3 antibody could be detected in 39.3% ( 2 ) and 64.9% ( 3 ) of 2,919 goat sera and 1,863 sheep sera, respectively, collected from six provinces in China. Moreover, CPIV3 genomes were frequently detected in clinical samples using real-time qPCR ( 4 ).…”

Section: Introductionmentioning

confidence: 98%

Bta-miR-98 Suppresses Replication of Caprine Parainfluenza Virus Type 3 Through Inhibiting Apoptosis by Targeting Caspase-3

Zhong

Liao

et al. 2020

Front. Immunol.

Self Cite

View full text Add to dashboard Cite

Caprine parainfluenza virus type 3 (CPIV3) is an emerging respiratory pathogen that affects the sheep and goat industry in China and possibly other countries around the world. Accumulating evidence suggests that microRNAs play important roles in regulating virus-host interactions and can suppress or facilitate viral replication. In this study, we showed that CPIV3 infection induced apoptosis in Madin-Darby bovine kidney (MDBK) cells, as determined by morphological changes and flow cytometry. Caspase activity and the expression of pro-apoptotic genes further indicated that CPIV3 induced apoptosis by activating both the intrinsic and extrinsic pathways. We also demonstrated the involvement of bta-microRNA-98 (bta-miR-98) in regulating CPIV3-induced apoptosis. Bta-miR-98 was downregulated in MDBK cells infected with CPIV3. Overexpression of bta-miR-98 significantly decreased the activities of caspase-3, −8, and −9. Conversely, inhibition of bta-miR-98 had completely opposite effects. Furthermore, our data showed that bta-miR-98 markedly affected CPIV3 replication by regulating apoptosis. Importantly, we found that bta-miR-98 modulated CPIV3-induced apoptosis by targeting caspase-3, an effector of apoptosis. Collectively, our results may suggest that CPIV3 infection induced apoptosis and downregulated the levels of bta-miR-98, and this miRNA regulated viral replication through effected apoptosis. This study contributes to our understanding of the molecular mechanisms underlying CPIV3 pathogenesis.

show abstract

Section: Introductionmentioning

confidence: 98%

Bta-miR-98 Suppresses Replication of Caprine Parainfluenza Virus Type 3 Through Inhibiting Apoptosis by Targeting Caspase-3

Zhong

Liao

et al. 2020

Front. Immunol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…CPIV3 causes severe respiratory diseases, characterized by the clinical symptoms as coughing, nasal discharges, viremia, virus shedding and lung consolidation [18]. The virus infects sheep and goats, and has been found in many provinces in China with an overall seroprevalence of 39.3-64.9 % [19,20]. The mechanism of pathogenesis and transmission of CPIV3 remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Exosomes promote caprine parainfluenza virus type 3 infection by inhibiting autophagy

Liang

et al. 2020

Journal of General Virology

Self Cite

View full text Add to dashboard Cite

Caprine parainfluenza virus type 3 (CPIV3) is a novel important pathogen causing respiratory disease in goats, but the pathogenic mechanism is not clear yet. Evidence suggests that exosomes transfer biologically active molecules between cells. Viral infections can cause profound changes in exosome components, and exosomes have been involved in viral transmission and pathogenicity. In this study, we explored the characteristics and functions of exosomes purified from the supernatant of Madin–Darby bovine kidney (MDBK) cells inoculated with CPIV3. Infection of CPIV3 showed increased exosome secretion and the loading of viral proteins and RNA into exosomes. These exosomes were capable of transferring CPIV3 genetic materials to recipient cells to establish a productive infection and promote the viral replication. To explore the potential mechanism, small RNA deep sequencing revealed that CPIV3 exosomes contained a diverse range of RNA species, including miRNA and piRNA, in proportions different from exosomes isolated from mock-infected cells. Expression patterns of 11 differentially expressed miRNAs were subsequently validated by quantitative reverse transcriptase PCR (qRT-PCR). Targets of miRNAs were predicted and functional annotation analysis showed that the main pathways involved were autophagy signalling ways. Autophagy inhibited by the CPIV3-exosome was further verified, and miR-126–3 p_2 packaged in the vesicles was an important regulation factor in this process. Inhibition of autophagy may be one of the responsible reasons for promoting efficient replication of exosome-mediated CPIV3 infection. The study suggests that exosomes are key in pathogenesis or protection against CPIV3. Further understating of their role in CPIV3 infection may bring novel insight to the development of protection measures.

show abstract

“…The comparison of complete L protein amino acid sequences, representing the current species demarcation criterion for Respirovirus genus [26], shows that ChamoisRV/IT2014 possesses sequence differences from the other respirovirus species. Moreover, although the phylogenetic analysis of ChamoisRV/IT2014 based on M, HN and F genes, used as targets for genetic typing of RV3 [5], confirms the highest nucleotide identity with CRV3, it shows low nucleotide identity for all three genes.…”

Section: Discussionmentioning

confidence: 85%

“…CRV3 (formerly CPIV3) was first identified in goats [2] and recently also in sheep in China [5]. To date, CRV3 has not been reported in other countries.…”

Section: Introductionmentioning

confidence: 99%

Identification and Genetic Characterization of a Novel Respirovirus in Alpine Chamois (Rupicapra rupicapra rupicapra)

Luzzago

Ebranati

Llombart‐Bosch

et al. 2020

Animals

View full text Add to dashboard Cite

Croatia. This wide distribution favours interactions with other wild ruminants and livestock, implying the risk of cross-transmission of pathogens. Due to the impact of lung diseases on chamois populations, the investigation of respiratory pathogens is important for wildlife conservation and for the understanding of infection transmission at the livestock-wildlife interface. In the present study, a novel respirovirus was isolated from a chamois with lung lesions and characterized by full-genome sequence and phylogeny. The genome characterization of this novel virus revealed similarities to domestic ruminant respiroviruses, mainly of caprine and ovine origin. Overall, phylogenetic analyses indicated that the chamois virus is distinct from already defined species and suggested that it is a putative novel member of the genus Respirovirus. The present investigation contributes to the knowledge of respiratory infections in wild ruminants and raises questions on the epidemiological link between chamois and other animal species.Abstract: The Respirovirus genus, family Paramamixoviridae, includes respiratory viral pathogens. Here we report the identification and genetic characterization of a respirovirus in an Alpine chamois showing interstitial pneumonia associated with catarrhal bronchopneumonia. The full-genome characterization of this respirovirus, named ChamoisRV/IT2014, revealed low similarities to caprine respirovirus (77.1%), bovine respirovirus (74.5%) and human respirovirus (72.0%). The phylogenetic analyses based on the full-length genome sequence of the novel isolate and reference respirovirus strains showed that ChamoisRV/IT2014 clustered with caprine respirovirus but formed a separate branch. The phylogenetic tree topology of complete large protein amino acid sequences, representing the current species demarcation criterion for Respirovirus genus, showed a 0.05 branch length of ChamoisRV/IT2014 sequence between the nearest node and the tip of the branch, suggesting that this virus belongs to a novel species. This new isolate in a new host species raises several questions to be addressed on the epidemiological role of chamois and the risks of cross-transmission between wild ruminants and livestock.

show abstract

Epidemiological investigation and phylogenetic analysis of caprine parainfluenza virus type 3 in sheep of China

Cited by 17 publications

References 13 publications

Bta-miR-98 Suppresses Replication of Caprine Parainfluenza Virus Type 3 Through Inhibiting Apoptosis by Targeting Caspase-3

Bta-miR-98 Suppresses Replication of Caprine Parainfluenza Virus Type 3 Through Inhibiting Apoptosis by Targeting Caspase-3

Exosomes promote caprine parainfluenza virus type 3 infection by inhibiting autophagy

Identification and Genetic Characterization of a Novel Respirovirus in Alpine Chamois (Rupicapra rupicapra rupicapra)

Contact Info

Product

Resources

About