Epidemiological Characteristics of Angular Leaf Spot of Bean: A Systems Analysis

Allorent, Delphine; Savary, Serge

doi:10.1007/s10658-005-4038-y

Cited by 19 publications

(10 citation statements)

References 20 publications

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“…Angular leaf spot (ALS) is among the most destructive diseases of common bean (Phaseolus vulgaris) in tropical and subtropical regions (Allorent and Savary, 2005). The disease is ranked second among the biotic factors that constrain bean production in Africa (Wangara et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Pathogen Variability and New Sources of Resistance to Angular Leaf Spot Among Bean Landraces in Uganda

Ddamulira¹

2019

AJFAND

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Angular leaf spot (ALS), a fungal disease caused by Pseudocercospora griseola, has a significant impact on bean productivity in Africa, causing up to 80% yield losses. Efforts to breed for ALS resistance are challenged by continuously evolving pathogen races that differ by location. Released bean varieties in Uganda are susceptible to ALS; the few exotic sources of resistance available are not well adapted to local environmental conditions. To overcome these challenges, a study was conducted to understand pathogen variability and identify new sources of ALS resistance for deployment in ALS resistance breeding. Variability in 45 P. griseola pathotypes was elucidated using a set of 12 ALS differential cultivars, random amplified microsatellite markers, and conserved sequences. The differentials and markers defined 12 pathotypes and 30 haplotypes, respectively, which belonged to the Middle American and Andean gene pool groups, each with high variability. Among the 74 bean landraces screened using the 1:6, 17:39, 21:39, and 61:63 P. griseola pathotypes, only U00279 showed consistent resistance to all the four pathotypes. U00297's resistance to pathotype 17:39 was conferred by a single dominant gene, while digenic epistatic gene interactions were responsible for resistance to other pathotypes. The dominant gene in U00297 was independent of resistance genes harbored by documented resistance sources AND277 and G5686. The results revealed high variability in P. griseola and identified a new source of broad ALS resistance. The divergent inheritance patterns of resistance to the different pathotypes indicate the importance of race specificity of the target host plant in breeding for disease resistance.

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Section: Introductionmentioning

confidence: 99%

Pathogen Variability and New Sources of Resistance to Angular Leaf Spot Among Bean Landraces in Uganda

Ddamulira¹

2019

AJFAND

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“…Phaeoisariopsis griseola (Sacc.) Ferraris) [ 10 ], and can be identified by angular necrotic spots on plant leaves and pods [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Association Studies of Anthracnose and Angular Leaf Spot Resistance in Common Bean (Phaseolus vulgaris L.)

et al. 2016

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The common bean (Phaseolus vulgaris L.) is the world’s most important legume for human consumption. Anthracnose (ANT; Colletotrichum lindemuthianum) and angular leaf spot (ALS; Pseudocercospora griseola) are complex diseases that cause major yield losses in common bean. Depending on the cultivar and environmental conditions, anthracnose and angular leaf spot infections can reduce crop yield drastically. This study aimed to estimate linkage disequilibrium levels and identify quantitative resistance loci (QRL) controlling resistance to both ANT and ALS diseases of 180 accessions of common bean using genome-wide association analysis. A randomized complete block design with four replicates was performed for the ANT and ALS experiments, with four plants per genotype in each replicate. Association mapping analyses were performed for ANT and ALS using a mixed linear model approach implemented in TASSEL. A total of 17 and 11 significant statistically associations involving SSRs were detected for ANT and ALS resistance loci, respectively. Using SNPs, 21 and 17 significant statistically associations were obtained for ANT and angular ALS, respectively, providing more associations with this marker. The SSR-IAC167 and PvM95 markers, both located on chromosome Pv03, and the SNP scaffold00021_89379, were associated with both diseases. The other markers were distributed across the entire common bean genome, with chromosomes Pv03 and Pv08 showing the greatest number of loci associated with ANT resistance. The chromosome Pv04 was the most saturated one, with six markers associated with ALS resistance. The telomeric region of this chromosome showed four markers located between approximately 2.5 Mb and 4.4 Mb. Our results demonstrate the great potential of genome-wide association studies to identify QRLs related to ANT and ALS in common bean. The results indicate a quantitative and complex inheritance pattern for both diseases in common bean. Our findings will contribute to more effective screening of elite germplasm to find resistance alleles for marker-assisted selection in breeding programs.

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“…P. griseola spores germinate on the leaf surface after 3 days of moist conditions, enter the leaf through the stomata and grow intercellularly, limited by the leaf veins resulting in an angular lesion shape (Monda et al 2001 ; Willocquet et al 2004 ). Infection and sporulation occurs in a broad temperature range, from 10 to 33 °C (reviewed in Allorent and Savary 2005 ). Following the two gene pools of common bean, Mesoamerican and Andean, the pathogen evolved in each gene pool separately (Guzman et al 1995 ; Crous et al 2006 ).…”

Section: Introductionmentioning

confidence: 99%

Fine-mapping of a major QTL controlling angular leaf spot resistance in common bean (Phaseolus vulgaris L.)

et al. 2015

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Key messageA major QTL for angular leaf spot resistance in the common bean accession G5686 was fine-mapped to a region containing 36 candidate genes. Markers have been developed for marker-assisted selection.AbstractCommon bean (Phaseolus vulgaris L.) is an important grain legume and an essential protein source for human nutrition in developing countries. Angular leaf spot (ALS) caused by the pathogen Pseudocercospora griseola (Sacc.) Crous and U. Braun is responsible for severe yield losses of up to 80 %. Breeding for resistant cultivars is the most ecological and economical means to control ALS and is particularly important for yield stability in low-input agriculture. Here, we report on a fine-mapping approach of a major quantitative trait locus (QTL) ALS4.1GS, UC for ALS resistance in a mapping population derived from the resistant genotype G5686 and the susceptible cultivar Sprite. 180 F3 individuals of the mapping population were evaluated for ALS resistance and genotyped with 22 markers distributed over 11 genome regions colocating with previously reported QTL for ALS resistance. Multiple QTL analysis identified three QTL regions, including one major QTL on chromosome Pv04 at 43.7 Mbp explaining over 75 % of the observed variation for ALS resistance. Additional evaluation of 153 F4, 89 BC1F2 and 139 F4/F5/BC1F3 descendants with markers in the region of the major QTL delimited the region to 418 kbp harboring 36 candidate genes. Among these, 11 serine/threonine protein kinases arranged in a repetitive array constitute promising candidate genes for controlling ALS resistance. Single nucleotide polymorphism markers cosegregating with the major QTL for ALS resistance have been developed and constitute the basis for marker-assisted introgression of ALS resistance into advanced breeding germplasm of common bean.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-015-2472-6) contains supplementary material, which is available to authorized users.

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Epidemiological Characteristics of Angular Leaf Spot of Bean: A Systems Analysis

Cited by 19 publications

References 20 publications

Pathogen Variability and New Sources of Resistance to Angular Leaf Spot Among Bean Landraces in Uganda

Pathogen Variability and New Sources of Resistance to Angular Leaf Spot Among Bean Landraces in Uganda

Genome-Wide Association Studies of Anthracnose and Angular Leaf Spot Resistance in Common Bean (Phaseolus vulgaris L.)

Fine-mapping of a major QTL controlling angular leaf spot resistance in common bean (Phaseolus vulgaris L.)

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