Epidemiological and molecular characterization of dengue viruses imported into Guangzhou during 2009–2013

Shi, Yongxia; Li, Shufen; Li, Xiaobo; Zheng, Kui; Yuan, Shuai; Huang, Jicheng

doi:10.1186/s40064-016-3257-3

Cited by 10 publications

(7 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our study further con rms the reports that the major epidemics thought to be dengue, emanated from three continents: Asia, Africa, and North America (42). There is a sudden increase in the number of imported DENV cases worldwide due to globalization (41). Adamawa is a state in Nigeria, a country located in West Africa region and shares a porous border with many African countries and accelerated in global trade and bilateral exchange program with many Asian countries such as China, India, Philippines, Malaysia, and Dubai.…”

Section: Discussionsupporting

confidence: 87%

Genetic diversity of Dengue virus serotypes circulating among Aedes mosquitoes in selected regions of Nigeria

Isa¹,

Ndams²,

Aminu³

et al. 2021

Preprint

View full text Add to dashboard Cite

The DENV mosquito vector is endemic to tropical and subtropical climates, placing ∼40% of the world’s population at direct risk of dengue infection. Currently, in Nigeria the status of DENV serotypes circulating among mosquitoes’ vectors is unknown. Our study was designed to identify and characterize the DENV serotypes circulating in Aedes mosquito populations collected in selected sites in Nigeria. The mosquitoes were collected, identified morphologically to species level using colored identification keys of Rueda. Generally, each species identified was tested in pools of 20 individuals of each Aedes species. RT-PCR and nested PCR were used to detected DENV serotypes in mosquitoes and characterized using Sanger sequencing methods. The results show that DENV serotypes were detected in 58.54% of the pools of mosquitoes screened. This corroborates with our unpublished report on the presence of DENV IgM antibodies in humans in the same study area. All DENV1-4 serotypes were detected in Ae. aegypti, Ae. albopictus and Ae. gaillosi with DENV4 serotype being reported for the first time in Nigeria. DENV2 (37.8%) was the most detected serotypes, while double and triple co-infections of serotypes were detected in 24.4% of the pools. Phylogenetic analysis revealed a strong evolutionary relatedness of DENV serotypes in our study with that of South and Southeast Asia, North America, and other African countries. This is the first reports on the co-infection of natural DENV in Aedes species pools in Nigeria. Thus, our study speculates a possible linkage between DENV serotypes and febrile flu-like disease burden being experienced by host communities in northern Nigeria.

show abstract

Section: Discussionsupporting

confidence: 87%

Genetic diversity of Dengue virus serotypes circulating among Aedes mosquitoes in selected regions of Nigeria

Isa¹,

Ndams²,

Aminu³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…These results show that for more than ten years, there has been a rapid turnover of clusters of DENV-1 genotype I isolates due to probable multiple introductions in the Lao PDR from neighboring countries following the trend of this DENV-1 pattern at the regional level [15,16,18,29,[36][37][38]. The new genetic data also allows access to a deeper level of analysis of DENV through the clusters' dynamics.…”

Section: Discussionmentioning

confidence: 79%

Using Background Sequencing Data to Anticipate DENV-1 Circulation in the Lao PDR

Calvez¹,

Bounmany²,

Balière

et al. 2021

Microorganisms

View full text Add to dashboard Cite

Since its first detection in 1979, dengue fever has been considered a major public health issue in the Lao People’s Democratic Republic (PDR). Dengue virus (DENV) serotype 1 was the cause of an epidemic in 2010–2011. Between 2012 and 2020, major outbreaks due successively to DENV-3, DENV-4 and recently DENV-2 have been recorded. However, DENV-1 still co-circulated in the country over this period. Here, we summarize epidemiological and molecular data of DENV-1 between 2016 and 2020 in the Lao PDR. Our data highlight the continuous circulation of DENV-1 in the country at levels ranging from 16% to 22% among serotyping tests. In addition, the phylogenetic analysis has revealed the circulation of DENV-1 genotype I at least since 2008 with a co-circulation of different clusters. Sequence data support independent DENV-1 introductions in the Lao PDR correlated with an active circulation of this serotype at the regional level in Southeast Asia. The maintenance of DENV-1 circulation over the last ten years supports a low level of immunity against this serotype within the Lao population. Thereby, the risk of a DENV-1 epidemic cannot be ruled out in the future, and this emphasizes the importance of maintaining an integrated surveillance approach to prevent major outbreaks.

show abstract

“…All DENV-1 isolates detected in this study belonged to genotype I, which was first documented in Thailand in 1981 43 . Since that time, DENV-1 genotype I has been routinely isolated in Thailand and several other countries in Southeast Asia 44 , 45 . Interestingly, our DENV-1 phylogenetic tree showed that previous and present genotype I Thai strains did not cluster in the same clade.…”

Section: Discussionmentioning

confidence: 99%

Molecular surveillance of arboviruses circulation and co-infection during a large chikungunya virus outbreak in Thailand, October 2018 to February 2020

Khongwichit

Chuchaona

Vongpunsawad

et al. 2022

Sci Rep

View full text Add to dashboard Cite

A large national outbreak of chikungunya virus (CHIKV) was recently reported in Thailand. While dengue virus (DENV) infection tends to occur year-round with an upsurge in the rainy season, Zika virus (ZIKV) also circulates in the country. The overlap in the distribution of these viruses increased the probability of co-infections during the heightened CHIKV activity. By examining 1806 patient serum samples submitted for CHIKV diagnostics from October 2018-February 2020 (511 CHIKV-negatives and 1295 CHIKV-positives), we used real-time reverse transcription-polymerase chain reaction to identify DENV and ZIKV individually. A total of 29 ZIKV and 36 DENV single-infections were identified. Interestingly, 13 co-infection cases were observed, of which 8 were CHIKV/DENV, 3 were CHIKV/ZIKV, and 2 were DENV/ZIKV. There were six DENV genotypes (13 DENV-1 genotype I, 10 DENV-2 Asian I, 10 DENV-2 Cosmopolitan, 6 DENV-3 genotype I, 2 DENV-3 genotype III, and 5 DENV-4 genotype I). Additionally, ZIKV strains identified in this study either clustered with strains previously circulating in Thailand and Singapore, or with strains previously reported in China, French Polynesia, and the Americas. Our findings reveal the co-infection and genetic diversity patterns of mosquito-borne viruses circulating in Thailand.

show abstract

Epidemiological and molecular characterization of dengue viruses imported into Guangzhou during 2009–2013

Cited by 10 publications

References 20 publications

Genetic diversity of Dengue virus serotypes circulating among Aedes mosquitoes in selected regions of Nigeria

Genetic diversity of Dengue virus serotypes circulating among Aedes mosquitoes in selected regions of Nigeria

Using Background Sequencing Data to Anticipate DENV-1 Circulation in the Lao PDR

Molecular surveillance of arboviruses circulation and co-infection during a large chikungunya virus outbreak in Thailand, October 2018 to February 2020

Contact Info

Product

Resources

About