Epidemiological and molecular analysis of hepatitis B virus infection in manicurists in Central Brazil

Cavaretto, Larissa dos Santos Pereira; Motta-Castro, Ana Rita Coimbra; Teles, Sheila Araújo; Souza, Fernanda Queiroz de; Cardoso, Wesley Márcio; Rezende, Grazielli Rocha de; Tanaka, Tayana Serpa Ortiz; Bandeira, Larissa Melo; Cesar, Gabriela Alves; Puga, Marco Antonio Moreira; Nepomuceno, Bruna B.; Lago, Bárbara Vieira do; Fernandes-Fitts, Sonia M.

doi:10.1002/jmv.24940

Cited by 2 publications

(1 citation statement)

References 36 publications

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“…Phylogenetic analysis demonstrated that samples from subgenotypes A1, D3, D4 and F2 clustered with circulating viral isolates [ 51 , 52 , 53 , 54 , 55 , 56 ]. On the other hand, samples from A2, D1 and D2 clustered with sequences from Europe [ 57 ], USA [ 58 ], South Africa (unpublished) and Turkey [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

Genomic Variability of Hepatitis B Virus Circulating in Brazilian Western Amazon

Roca

Villar

Lima

et al. 2022

Viruses

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The emergence of clinically relevant mutations in the hepatitis B virus (HBV) genome has been a matter of great debate because of the possibility of escape from the host’s immune system, the potential to cause more severe progression of liver diseases and the emergence of treatment-resistant variants. Here we characterized the circulating variants of HBV in Rondônia State, in the north of Brazil. Serum samples of 62 chronic HBV carriers were subjected to PCR assays and clinical data were collected. Mutations and genotypes were characterized through direct sequencing. The findings show the presence of subgenotypes A1 (54.83%, 34/62), D3 (16.13%, 10/62), F2 (16.13%, 10/62), A2 (4.84%, 3/62), D2 (3.23%, 2/62), D1 (1.61%, 1/62), D4 (1.61%, 1/62) and F4 (1.61%, 1/62). Deletions in the pre-S2 region were found in 13.79% (8/58) of the samples, mutations in the S gene in 59.68% (37/62) and RT mutations in 48.39% (30/62). We found a variable genotypic distribution in different locations and important mutations related to immune escape and drug resistance in Western Amazonia, which contributed to genetic surveillance and provided important information to help control the disease.

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Section: Discussionmentioning

confidence: 99%

Genomic Variability of Hepatitis B Virus Circulating in Brazilian Western Amazon

Roca

Villar

Lima

et al. 2022

Viruses

View full text Add to dashboard Cite

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Genetic diversity of hepatitis B virus quasispecies in different biological compartments reveals distinct genotypes

do Lago,

Bezerra,

Moreira

et al. 2023

Sci Rep

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The selection pressure imposed by the host immune system impacts hepatitis B virus (HBV) quasispecies variability. This study evaluates HBV genetic diversity in different biological fluids. Twenty paired serum, oral fluid, and DBS samples from chronic HBV carriers were analyzed using both Sanger and next generation sequencing (NGS). The mean HBV viral load in serum was 5.19 ± 4.3 log IU/mL (median 5.29, IQR 3.01–7.93). Genotype distribution was: HBV/A1 55% (11/20), A2 15% (3/20), D3 10% (2/20), F2 15% (3/20), and F4 5% (1/20). Genotype agreement between serum and oral fluid was 100% (genetic distances 0.0–0.006), while that between serum and DBS was 80% (genetic distances 0.0–0.115). Two individuals presented discordant genotypes in serum and DBS. Minor population analysis revealed a mixed population. All samples displayed mutations in polymerase and/or surface genes. Major population analysis of the polymerase pointed to positions H122 and M129 as the most polymorphic (≥ 75% variability), followed by V163 (55%) and I253 (50%). Neither Sanger nor NGS detected any antiviral primary resistance mutations in the major populations. Minor population analysis, however, demonstrated the rtM204I resistance mutation in all individuals, ranging from 2.8 to 7.5% in serum, 2.5 to 6.3% in oral fluid, and 3.6 to 7.2% in DBS. This study demonstrated that different fluids can be used to assess HBV diversity, nonetheless, genotypic differences according to biological compartments can be observed.

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Epidemiological and molecular analysis of hepatitis B virus infection in manicurists in Central Brazil

Cited by 2 publications

References 36 publications

Genomic Variability of Hepatitis B Virus Circulating in Brazilian Western Amazon

Genomic Variability of Hepatitis B Virus Circulating in Brazilian Western Amazon

Genetic diversity of hepatitis B virus quasispecies in different biological compartments reveals distinct genotypes

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