Epidemiological and Microbiological Profile of Infective Keratitis in a Tertiary Care Centre, South India

Jampala, Sandhya; Gopinathan, Anusha; Aparna,; Nair, Deepthi; Kr, Dinesh; Radhakrishnan, Ashwin; Karim, Sabrina

doi:10.15272/ajbps.v4i37.616

Cited by 3 publications

(4 citation statements)

References 19 publications

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“…In the present study, the sensitivity using KOH wet mount was 54.55% (Table 2), in a study conducted by Jampala et al (33), the sensitivity of KOH wet mount was reported to be 33.33%. Positivity of CFW plus KOH wet mount in the present study is in line with a study conducted by Shokohi et al (17) (31.81%) and the experimental proof of producing corneal ulcer after the injection of fungi and showed that corticosteroids enhance the virulence.…”

Section: Resultssupporting

confidence: 55%

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A Comparative Study of Potassium Hydroxide Wet Mount, Calcofluor White Staining and Culture for the Diagnosis of Keratomycosis

Alekhya

Sunder

Prathiba

2020

Avicenna J Clin Microbiol Infect

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Background: The incidence of keratomycosis has increased dramatically in recent years. Early diagnosis and treatment of keratomycosis are important in preventing further complications. Direct microscopic techniques are time-saving for diagnosing keratomycosis when compared to culture methods. This study was carried out to determine the sensitivities of potassium hydroxide (KOH) wet mount, Gram stain and Calcofluor white (CFW) stain plus KOH wet mount by taking culture as the gold standard. Methods: Corneal scrapings were collected from 150 clinically suspected patients with keratomycosis. Demographic profile was collected and analyzed. Results: Of these patients, 67.33% were male, 24% were in the age group of 51-60 years, 70% were ruralresidents, 44% were agricultural workers, and 60% presented with a history of corneal trauma. Laboratory investigations have revealed that 29.33% (44 cases) were culture positive. In other words, Fusarium spp. was isolated in 17 cases, Aspergillus spp. in 14 cases, phaeoid fungi in 3 cases and unidentified fungi in 10 cases. The positivity of CFW stain plus KOH wet mount, KOH wet mount, and Gram stain was 30%, 23.3%, and 20%, respectively. Sensitivities of CFW stain plus KOH wet mount, KOH wet mount, and Gram stain were 79.55%, 54.55%, and 47.62%, respectively. Conclusion: Post-investigative analysis has revealed that CFW stain plus KOH wet mount was better than KOH wet mount alone in demonstrating fungal pathogens. Therefore, early diagnosis of keratomycosis by meticulous examination of corneal scrapings by direct microscopy specifically using CFW stain plus KOH wet mount and institution of antifungal therapy may limit ocular morbidity and disastrous sequelae among these patients.

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Section: Resultssupporting

confidence: 55%

“…was the most common isolate (3,14,19,(29)(30)(31)(32). While Jampala et al (33) reported Candida spp. as the most common isolate.…”

Section: Resultsmentioning

confidence: 98%

A Comparative Study of Potassium Hydroxide Wet Mount, Calcofluor White Staining and Culture for the Diagnosis of Keratomycosis

Alekhya

Sunder

Prathiba

2020

Avicenna J Clin Microbiol Infect

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“…According to a study by Jampala, et al 11 in 49 patients, among ocular disorders, lid disorders were the primary causes with blepharitis 7% among the study subjects.…”

Section: Discussionmentioning

confidence: 92%

“…[8][9][10] According to the WHO, corneal blindness attributed to infectious keratitis is a leading reason for visual disability and it is classified as a 'silent epidemic' taking place all over the world. 11 Keratitis is considered an ocular emergency requiring immediate and appropriate anti-microbial therapy to prevent permanent vision loss. So, if left untreated, it can have severe consequences.…”

Section: Introductionmentioning

confidence: 99%

Etiological factors & microbiological characteristics of infectious keratitis in western Maharashtra

Mohan

Magdum²,

Kaul³

et al. 2021

IJCEO

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To identify the etiological factors and study the microbiological characteristics of infectious keratitis in Western Maharashtra. This was a prospective observational hospital‐based study conducted at the Department of Ophthalmology, at a tertiary care hospital, in a part of central India. Data related to socioeconomic status and etiological factors was collected. Results were analyzed on the basis of history, slit lamp examination, microbiological scraping and culture results. During the period of September 2018 to August 2020, a total of 60 patients with infectious keratitis were examined in the Ophthalmology OPD. Majority of patients of infectious keratitis were between 61 and 70 (18.33%) age group. Incidence in male was higher (68%) than female. Ocular trauma was the most common etiological factor (52%). Prevalence of Fungal keratitis (52%) was higher than bacterial (46%) in this part of India. 57% subjects showed organism on Gram staining. 53% subjects showed KOH positivity. 68.3% showed growth over microbiological culture with the most common species isolated as Fusarium species (26.6%) followed by Pseudomonas aeruginosa. Incidence of fungal keratitis is higher in this part of Western Maharashtra. Most common etiological factor was determined to be trauma with vegetative matter among farmers which tells us that they are most prone for infectious keratitis. Prompt diagnosis, microbiological scraping, culture growth identification and starting on appropriate treatment can help reduce the morbidity of corneal ulcers.

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Microbiological spectrum of ocular infections in patients of tertiary care eye hospital of Punjab

Oberoi¹,

Singh

Singh³

et al. 2021

IJMMTD

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Eye is a complex and sensitive organ and is therefore more vulnerable to trauma and various infections ocular infections are one of the most commonly encountered infections. Any part of the eye can be infected by bacteria, fungi, parasites or viruses. In order to minimize the ocular morbidity and mortality, timely antimicrobial treatment must be initiated on the basis of clinical and microbial evaluation. For specific antibacterial treatment, isolation and identification of bacterial pathogens along with antibiotic susceptibility spectrum is essential. Hence, this study was undertaken to isolate and identify the bacterial and fungal pathogens responsible for the development of ocular infections and to determine their in vitro susceptibilities to commonly used antibiotics. A total of 203 samples of suspected ocular infections comprising 117 conjunctival swabs, 34 corneal specimens, 24 lacrimal pus samples and 28 vitreous specimens were analysed. Out of them 102(50.1%) showed growth. 82(40%) were bacterial and 21(10.3%) were fungal isolates. Due to the emergence of drug resistance it is imperative that all ophthalmological samples must be tested for antibiotic resistance as far as possible. Indiscriminate use of over the counter antibiotic eye formulations should be avoided and also it is important to know the changing pattern of pathogens.

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Epidemiological and Microbiological Profile of Infective Keratitis in a Tertiary Care Centre, South India

Cited by 3 publications

References 19 publications

A Comparative Study of Potassium Hydroxide Wet Mount, Calcofluor White Staining and Culture for the Diagnosis of Keratomycosis

A Comparative Study of Potassium Hydroxide Wet Mount, Calcofluor White Staining and Culture for the Diagnosis of Keratomycosis

Etiological factors & microbiological characteristics of infectious keratitis in western Maharashtra

Microbiological spectrum of ocular infections in patients of tertiary care eye hospital of Punjab

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