Epidemiological Analysis of Salmonella Enteritidis Isolates Using Pulsed-Field Gel Electrophoresis and Bacteriophage Typing over the Period of April 2000 to March 2003 in Gifu Prefecture

Itagaki, Michiyo; Shiraki, Yutaka; Yamada, Mika; Tokoro, Mitsuo; Izumiya, Hidemasa; Watanabe, Haruo

doi:10.11150/kansenshogakuzasshi1970.78.690

Cited by 2 publications

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“…Enteritidis is the top serovar associated with human salmonellosis cases in Japan [16]. S. Enteritidis phage type 1 was commonly associated with these human salmonellosis outbreaks in Japan [6,12,14]. In this study, the same phage type (PT 1) was detected in S. Enteritidis isolates from rodents, an egg processing facility and liquid egg.…”

Section: Enteritidis Contamination In the Fieldsupporting

confidence: 50%

An Epidemiological Analysis of Salmonella Enteritidis Contamination in a Rat-Infested Chicken Layer Farm, an Egg Processing Facility, and Liquid Egg Samples by Pulsed-Field Gel Electrophoresis

Lapuz

Tani

Sasai

et al. 2007

The Journal of Veterinary Medical Science

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ABSTRACT. In order to determine the epidemiological link between the Salmonella Enteritidis contamination in a rat-infested chicken layer farm, an attached egg processing facility and liquid egg samples, several S. Enteritidis isolates were analyzed by pulsed-field gel electrophoresis (PFGE) and bacteriophage typing. A total of 33 S. Enteritidis strains were isolated from a total of 4,081 samples. Similar pulsed-field patterns were generated by S. Enteritidis isolates from liquid eggs, rats and effluent water. Additionally, only two phage types were detected among the S. Enteritidis isolates, PT 1b and PT 6. These results suggest that S. Enteritidis isolates from rats, egg processing facility, and liquid eggs are genetically related. Furthermore, S. Enteritidis infection in rats in layer farms poses a serious public health concern and should be included in future epidemiological studies. KEY WORDS: layer farm, rat, Salmonella Enteritidis.J. Vet. Med. Sci. 69(6): 649-652, 2007 Human illness caused by infection with Salmonella enterica subsp. enterica serovar Enteritidis increased worldwide beginning as early as 1970s and by 1990, became the primary cause of salmonellosis in the world [2]. A major factor in this increase has been attributed to consumption of undercooked eggs and egg products such as liquid egg, contaminated with S. Enteritidis [4]. Rodents, dirty environment, recent poultry farming practices, chicks, pullets, and feeds have all been considered as major sources for contamination with S. Enteritidis in chicken layer farms [5,7,[17][18][19]. In Japan, liquid egg is commonly used in making egg custard products. Murakami et al. [13] were able to isolate S. Enteritidis from unpasteurized liquid egg and epidemiologically linked it to isolates from human salmonellosis outbreaks. But little is known of the origin and mechanism of S. Enteritidis contamination in liquid egg.DNA-based typing by pulsed-field gel electrophoresis (PFGE) has been widely used as a molecular fingerprinting technique for the epidemiological analysis of bacterial infections. In this study, several S. Enteritidis isolates obtained from a rat-infested chicken layer farm, its attached egg processing facility and liquid egg samples from a liquid egg factory for 2 years were characterized by PFGE. The purpose of this study was to determine if there are epidemiological links between S. Enteritidis isolates obtained from layer houses, egg processing facility and liquid egg samples and more importantly to determine the mechanism of S. Enteritidis contamination in the field.A commercial chicken layer farm and an attached eggprocessing facility (GP center) were sampled regularly for S. Enteritidis contamination monitoring for 2 years. Samples for Salmonella isolation were 81 batches (6,480 eggs) of dirty and broken eggs, 130 batches (3,900 eggs) of nest run eggs, 720 environmental samples from the layer houses and 820 environmental samples from GP center, 1,436 samples of GP effluent water, 180 batches of rat fecal samples, and 87 rats. The...

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Section: Enteritidis Contamination In the Fieldsupporting

confidence: 50%

An Epidemiological Analysis of Salmonella Enteritidis Contamination in a Rat-Infested Chicken Layer Farm, an Egg Processing Facility, and Liquid Egg Samples by Pulsed-Field Gel Electrophoresis

Lapuz

Tani

Sasai

et al. 2007

The Journal of Veterinary Medical Science

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“…PT 21 has been detected in isolate from humans and poultry products between 1995 and 1997 (Nunes et al, 2003). Whereas PT1 is the most prevalent PT in Italy (Nastasi et al, 1997), Denmark (Baggesen et al, 1997), Finland (Lukinmaa et al, 2006), Spain (Soler et al, 2006), and Japan (Itagaki et al, 2004).…”

Section: Pfge and Ptmentioning

confidence: 97%

Phage types and pulsed-field gel electrophoresis patterns of Salmonella enterica serovar Enteritidis isolated from humans and chickens

Kim

Chun

et al. 2008

J Microbiol.

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We analyzed 66 Salmonella Enteritidis isolates in 2002. Thirty isolates were obtained from human patients with diarrhea, and 36 were obtained from chickens. A total of ten phage types (PT) were identified in the human and chicken isolates. PT1 and PT21 were the predominant PTs in both the human (20% and 13%) and chicken (17% and 47%) isolates. Twelve pulsotypes were generated by PFGE and divided into two major groups. Most of the PFGE types were categorized into cluster group 1. Eighteen chicken isolates in cluster group 1 showed high-level genetic association (>95%) with 22 other human isolates. Additionally, six chicken isolates from cluster group 2 showed fairly high-level genetic association (>95%) with the other seven human isolates. The highest levels of genetic association in humans and chickens were seen with A5-PT21 (11 isolates), A2-PT1 (7 isolates), and B1-PT4 (6 isolates). The Pulsed-Field Gel Electrophoresis (PFGE) and phage typing provided conclusive evidence that human Salmonella infections are attributable to the consumption of contaminated chicken.

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Epidemiological Analysis of Salmonella Enteritidis Isolates Using Pulsed-Field Gel Electrophoresis and Bacteriophage Typing over the Period of April 2000 to March 2003 in Gifu Prefecture

Cited by 2 publications

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An Epidemiological Analysis of Salmonella Enteritidis Contamination in a Rat-Infested Chicken Layer Farm, an Egg Processing Facility, and Liquid Egg Samples by Pulsed-Field Gel Electrophoresis

An Epidemiological Analysis of Salmonella Enteritidis Contamination in a Rat-Infested Chicken Layer Farm, an Egg Processing Facility, and Liquid Egg Samples by Pulsed-Field Gel Electrophoresis

Phage types and pulsed-field gel electrophoresis patterns of Salmonella enterica serovar Enteritidis isolated from humans and chickens

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