Epidemiological Analysis of
            <i>Salmonella enteritidis</i>
            Isolates from Humans and Broiler Chickens in Thailand by Phage Typing and Pulsed-Field Gel Electrophoresis

Boonmar, Sumalee; Bangtrakulnonth, Aroon; Pornrunangwong, Srirat; Terajima, Jun; Watanabe, Haruo; Kaneko, Ken‐ichi; Ogawa, Masahiro

doi:10.1128/jcm.36.4.971-974.1998

Cited by 56 publications

(20 citation statements)

References 25 publications

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“…This confirmed the high genetic homogeneity among the Salm. Enteritidis strains reported previously by other laboratories (Millemann et al 1995;Thong et al 1995;Landeras et al 1996;Boonmar et al 1998;Bakeri et al 2003). Plasmid profile analysis has been used as a rapid and useful method for subtyping of some Salmonella serotypes including Salm.…”

Section: Combination Of Pfge Plasmid Profile and Phage Typesmentioning

confidence: 99%

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Pulsed-field gel electrophoresis, plasmid profiles and phage types for the human isolates of Salmonella enterica serovar Enteritidis obtained over 13 years in Taiwan

et al. 2005

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Aims: Plasmid profile, phage typing, and pulsed-field gel electrophoresis (PFGE) patterns of 124 Salmonella Enteritidis strains isolated in 1998-2002 in Taiwan were analysed and the results were compared with those of the 63 strains obtained in 1991-1997, so that molecular subtypes and epidemic strains for Salmonella Enteritidis over a 13-year period (1991-2002) could be elucidated. Methods and Results: A total of 124 strains of Salmonella Enteritidis isolated from human in Taiwan between 1998 and 2002 were analysed by PFGE, plasmid analysis and phage typing. The results obtained were compared with those of the 63 strains obtained in 1991-1997, so that the clonal relationships for a total of 187 strains obtained over 13 years could be elucidated. For PFGE, restriction enzymes XbaI, SpeI and NotI were used for chromosomal DNA digestion. Results showed 28 PFGE pattern combinations for the 187 Salmonella strains. Of them, pattern X3S3N3 was the major subtype as 130 strains isolated from different locations during [1991][1992][1993][1994][1995][1996][1997][1998][1999][2000][2001][2002] showed this PFGE pattern. For all these 187 strains, the genetic similarity was higher than 80%. Plasmid analysis showed 17 distinct types, which consist of one to four plasmids and the predominant phage type of those strains was PT4 (71AE6%) and PT6a (13AE4%). The three methods identified different degrees of polymorphism in the following order: plasmid profile (18 types, D ¼ 0AE659) > PFGE (28 types, D ¼ 0AE512) > phage typing (13 types, D ¼ 0AE438). As PFGE patterns, phage type and plasmid profile were combined for subtyping, the 187 strains could be grouped into 46 subtypes and the discriminatory index was raised to 0AE795. For these 46 subtypes, the predominant one was X3S3N3/P1/PT4, which contained 77 (41%) isolates. Conclusions: Most of the Salmonella Enteritidis strains from sporadic cases were with pattern X3S3N3. They were the prevalent and may be the epidemic strains found in Taiwan during 1991-2002. The present study suggested that the several variants were derived from a single clonal line and the genome for strains of Salmonella Enteritidis are highly conserved over a 13-year period (1991)(1992)(1993)(1994)(1995)(1996)(1997)(1998)(1999)(2000)(2001)(2002). Significance and Impact of the Study: The results obtained here are useful for epidemiolgical study of salmonellosis caused by Salmonella Enteritidis in Taiwan. Comparing the data of the present study with those obtained for strains from other countries, the major subtypes for Salmonella Enteritidis infection in the world can be elucidated.

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Section: Combination Of Pfge Plasmid Profile and Phage Typesmentioning

confidence: 99%

“…It may cause salmonellosis and gastroenteritis in humans and animals. Animals and their products, especially meat and eggs from chickens, are considered as a major source of infection with this pathogen for humans (Rodrigue et al 1990;Boonmar et al 1998). In the past 10 years, Salm.…”

Section: Introductionmentioning

confidence: 99%

Pulsed-field gel electrophoresis, plasmid profiles and phage types for the human isolates of Salmonella enterica serovar Enteritidis obtained over 13 years in Taiwan

et al. 2005

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“…These methods include phage typing, serotyping, IS 200 typing, ribotyping, multilocus sequence typing, pulsed-field gel electrophoresis (PFGE), comparison of antibiograms, plasmid profiles, random amplified polymorphic DNAs and amplified fragment length polymorphisms (Helmuth & Schroeter, 1994;Olsen et al, 1994;Laconcha et al, 1998;Kotetishvili et al, 2002;Yan et al, 2004). Of these methods, PFGE is the most commonly used method for subtyping Salmonella strains (Thong et al, 1995;Laconcha et al, 1997;Boonmar et al, 1998;Fernandez et al, 2003). For phage typing, the major phage types of S. Enteritidis strains from different geographical areas have been found to vary.…”

Section: Introductionmentioning

confidence: 99%

The presence of major world-wide clones for phage type 4 and 8Salmonella entericaserovar Enteritidis and the evaluation of their virulence levels by invasiveness assaysin vitroandin vivo

Pang

Lin²,

Tsai

et al. 2006

FEMS Microbiology Letters

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Seventy-seven animal isolates of Salmonella enterica serovar Enteritidis (S. Enteritidis) obtained from the United States were analyzed by phage typing and pulsed field gel electrophoresis (PFGE). Thirty-nine strains were found with phage types (PT) 4, 8, and 13a. When the chromosomal DNA of these 39 isolated strains with PT4, 8, and 13a were digested with XbaI, SpeI and NotI, followed by PFGE analysis, 28 strains were found with a pattern combination of X4S4N4, which was the major subtype. When PFGE patterns of the US isolates with PT 4 and 8 were compared with those of the Taiwanese and German isolates, pattern X3S3N3 was confirmed to be the world-wide subtype shared by PT 4 isolates, as previously reported, while pattern X4S4N4 was newly found to be the most common subtype shared by PT 8 strains. The presence of such major world-wide clones, however, does not necessarily mean that these clones are highly virulent, at least not according to the results of invasiveness assays using cultured human intestinal epithelium cell line Int-407 and living BALB/mice.

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“…for humans. Studies that compare the genetic profiles of isolates obtained from the chicken processing chain with the genetic profiles of isolates obtained from clinical samples must be hereafter performed, as has been described in some studies (Boonmar et al 1998;Cardinale et al 2005;Cheong et al 2007).…”

Section: Resultsmentioning

confidence: 99%

Molecular tracking of Salmonella spp. in chicken meat chain: from slaughterhouse reception to end cuts

et al. 2015

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Due to the importance of Salmonella spp. in poultry products, this study aimed to track its main contamination routes since slaughtering reception to processing of chicken end cuts. Samples from different steps of slaughtering and processing (n = 277) were collected from two chicken slaughterhouses (Sl1 and Sl2) located in Minas Gerais state, Brazil, and subjected to Salmonella spp. detection. The obtained isolates were subjected to serological identification and tested by PCR for specific Salmonella spp. genes (ompC and sifB). Also, Salmonella spp. isolates were subjected to XbaI macrorestriction and pulsed-field gel electrophoresis (PFGE). Sixty-eight samples were positive for Salmonella spp. and 172 isolates were obtained. Sl1 and Sl2 presented similar frequencies of Salmonella spp. positive samples during reception, slaughtering and processing (p > 0.05), except for higher frequencies in Sl1 for chicken carcasses after de-feathering and evisceration (p < 0.05). PFGE allowed the identification of cross contamination and persistence of Salmonella spp. strains in Sl1. The results highlighted the relevance of the initial steps of chicken slaughtering for Salmonella spp. contamination, and the pre-chilling of carcasses as an important controlling tool. In addition, the presence of Salmonella spp. in chicken end cuts samples represents a public health concern.

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Epidemiological Analysis of Salmonella enteritidis Isolates from Humans and Broiler Chickens in Thailand by Phage Typing and Pulsed-Field Gel Electrophoresis

Cited by 56 publications

References 25 publications

Pulsed-field gel electrophoresis, plasmid profiles and phage types for the human isolates of Salmonella enterica serovar Enteritidis obtained over 13 years in Taiwan

Pulsed-field gel electrophoresis, plasmid profiles and phage types for the human isolates of Salmonella enterica serovar Enteritidis obtained over 13 years in Taiwan

The presence of major world-wide clones for phage type 4 and 8Salmonella entericaserovar Enteritidis and the evaluation of their virulence levels by invasiveness assaysin vitroandin vivo

Molecular tracking of Salmonella spp. in chicken meat chain: from slaughterhouse reception to end cuts

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