Epidemiological Analysis of a Herd Infected with Strangles by Imported Carrier Horse

Katayama, Masakazu; Miyama, M; Furuya, Satoko; Kuwamoto, Yasushi; Hobo, Seiji; Anzai, Toru

doi:10.12935/jvma1951.56.139

Cited by 8 publications

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“…Convalescent sera were obtained from 25 horses of uncertain age that had recovered from strangles (Katayama and others 2003). During quarantine inspection of nine quarter horses imported from the usa in January 2001, fever and enlarged submandibular lymph nodes were observed.…”

Section: Methodsmentioning

confidence: 99%

“…S equi infects the horses' cranial lymph nodes and is highly communicable to other horses. Outbreaks of strangles have been reported in many countries (Newton and others 1999, Katayama and others 2003, Gronbaek and others 2006, Kelly and others 2006) and can cause scheduled horse racing meets to be cancelled. An accurate diagnosis of the disease is indispensable to prevent it from spreading.…”

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confidence: 99%

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Proline‐glutamic acid‐proline‐lysine repetition peptide as an antigen for the serological diagnosis of strangles

2008

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The reactivity of the proline-glutamic acid-proline-lysine (PEPK) repetition peptide antigen in 3176 serum samples was investigated to evaluate its utility as an antigen for the serological diagnosis of strangles. The reactivity of the sera of horses infected with Streptococcus equi subspecies equi was high when the peptide had several PEPK repetitions. However, as the number of PEPK repetitions increased, the reactivity of the antigen with the sera of horses infected with Streptococcus equi subspecies zooepidemicus also increased. In horses infected experimentally with S equi, the reactivity of the PEPK antigen with five repetitions increased one week after inoculation and continued to increase during the following four weeks. The optical density (OD) values of test sera from horses infected experimentally with S equi and sera from horses that had recovered from strangles were high. The od values of sera from horses that had recovered from an experimental infection with S zooepidemicus and of sera from healthy horses were comparatively low.

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Section: Methodsmentioning

confidence: 99%

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Proline‐glutamic acid‐proline‐lysine repetition peptide as an antigen for the serological diagnosis of strangles

2008

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“…equi (S. equi) and is a commonly diagnosed and important infectious disease of horses worldwide [2,[6][7][8][13][14][15][16]. It is characterized by a mucopurulent nasal discharge and acute swelling with subsequent abscess formation in the submandibular and retropharyngeal lymph nodes [5,[13][14][15].…”

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confidence: 99%

“…S. equi infects the horse's cranial lymph glands and is highly communicable to other horses. Outbreaks of strangles have been reported in many countries and can cause scheduled horse racing meets to be cancelled [4,[6][7][8]11]. S. equi produce an M-like protein, SeM, and the seM gene has been proposed as a target gene to distinguish S. equi from different strains of streptococci including Streptococcus equi subsp.…”

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confidence: 99%

Development and Application of Loop-Mediated Isothermal Amplification Methods Targeting the seM Gene for Detection of Streptococcus equi subsp. equi

Hobo

Niwa

Oku

2012

The Journal of Veterinary Medical Science

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ABSTRACT. Loop-mediated isothermal amplification (LAMP) constitutes a potentially valuable diagnostic tool for rapid diagnosis of contagious diseases. In this study, we developed a novel LAMP method (seM-LAMP) to detect the seM gene of Streptococcus equi subsp. equi (S. equi), the causative agent of strangles in equids. The seM-LAMP successfully amplified the target sequence of the seM gene at 63C within 60 min. The sensitivity of the seM-LAMP was slightly lower than the 2nd reaction of the seM semi-nested PCR. To evaluate the species specificity of the seM-LAMP, we tested 100 S. equi and 189 non-S. equi strains. Significant amplification of the DNA originating from S. equi was observed within 60 min incubation, but no amplification of non-S. equi DNA occurred. The results were identical to those of seM semi-nested PCR. To investigate the clinical usefulness of the methods, the seM-LAMP and the seM semi-nested PCR were used to screen 590 nasal swabs obtained during an outbreak of strangles. Both methods showed that 79 and 511 swabs were S. equi positive and negative, respectively, and the results were identical to those of the culture examination. These results indicate that the seM-LAMP is potentially useful for the reliable routine diagnosis of Streptococcus equi subsp. equi infections.

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“…This disease has long been known as an important infectious disease of horses, and has occurred in almost all the countries, including Japan. It still causes economic losses in terms of the cost of treatment, quarantine measures and, occasionally, the death of animals [3,6,[10][11][12].…”

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Development of a Diagnostic Shuttle PCR Test Targeting the Streptococcus equi SeM Gene

Anzai

Hobo

Niwa

2006

JES

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Epidemiological Analysis of a Herd Infected with Strangles by Imported Carrier Horse

Cited by 8 publications

References 0 publications

Proline‐glutamic acid‐proline‐lysine repetition peptide as an antigen for the serological diagnosis of strangles

Proline‐glutamic acid‐proline‐lysine repetition peptide as an antigen for the serological diagnosis of strangles

Development and Application of Loop-Mediated Isothermal Amplification Methods Targeting the <i>seM</i> Gene for Detection of <i>Streptococcus equi</i> subsp. <i>equi</i>

Development of a Diagnostic Shuttle PCR Test Targeting the Streptococcus equi SeM Gene

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