Epidemiologic characterization of Pseudomonas aeruginosa in patients with cystic fibrosis

Spencker, F.-B.; Haupt, S.; Claros, Marina C.; Walter, S.; Lietz, T.; Schille, R.; Rodloff, Arne C.

doi:10.1046/j.1469-0691.2000.00171.x

Cited by 44 publications

(39 citation statements)

References 21 publications

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“…Stored isolates from the 100 patients were subject to molecular typing by PFGE, performed by a scientist blinded to clinical status, using the methods previously described by SPENCKER et al [4]. Plugs were digested with the restriction enzyme SpeI and electrophoresis was performed with a clamped homogenous electric fields-DRIII module (Bio-Rad Laboratories, Hercules, CA, USA) with modification of the switch times.…”

Section: Microbiologymentioning

confidence: 99%

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Clonal strains of Pseudomonas aeruginosa in paediatric and adult cystic fibrosis units

O’Carroll¹,

Syrmis²,

Wainwright³

et al. 2004

European Respiratory Journal

103

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Despite recent reports of clonal strains of Pseudomonas aeruginosa in cystic fibrosis (CF) units, the need for routine microbiological surveillance remains contentious.Sputum was collected prospectively from productive patients attending the regional paediatric and adult CF units in Brisbane, Australia. All P. aeruginosa isolates were typed using pulsed-field gel electrophoresis. Spirometry, anthropometrics, hospitalisations and antibiotic sensitivity data were recorded.The first 100 sputum samples (first 50 patients at each clinic) harboured 163 isolates of P. aeruginosa. A total of 39 patients shared a common strain (pulsotype 2), 20 patients shared a strain with at least one other patient and 41 patients harboured unique strains. Eight patients shared a strain identical to a previously reported Australian transmissible strain (pulsotype 1). Compared with the unique strain group, patients harbouring pulsotype 2 were younger and had poorer lung function. Treatment requirements were similar in these two groups, as were the rates of multiresistance.In conclusion, 59% of patients harboured a clonal strain, supporting the need for routine microbiological surveillance. In contrast to previously described clonal strains, the dominant pulsotype was indistinguishable from nonclonal strains with respect to both colonial morphology and multiresistance. The clinical significance of clonal strains remains uncertain and requires longitudinal study.

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Section: Microbiologymentioning

confidence: 99%

“…Once established in the CF lung, P. aeruginosa is virtually impossible to eradicate. Although the age of P. aeruginosa acquisition is an important determinant of prognosis [2], transmission from one patient to another is historically thought to be an uncommon occurrence [3,4] and, accordingly, segregation based on infection status is not routine.…”

mentioning

confidence: 99%

Clonal strains of Pseudomonas aeruginosa in paediatric and adult cystic fibrosis units

O’Carroll¹,

Syrmis²,

Wainwright³

et al. 2004

European Respiratory Journal

103

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“…Five colonies per patient were typed. PFGE of SpeI-genomic DNA digests of P. aeruginosa isolates was also performed [28].…”

mentioning

confidence: 99%

Pseudomonas aeruginosa transmission is infrequent in New Zealand cystic fibrosis clinics

Schmid¹,

Ling²,

Leung³

et al. 2008

European Respiratory Journal

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Pseudomonas aeruginosa is an important pathogen in cystic fibrosis (CF). Although most patients harbour unique P. aeruginosa isolates, some clinics report patients sharing common strains. The overall importance of person-to-person transmission in P. aeruginosa acquisition and whether routine patient segregation is necessary remains uncertain. The present authors therefore investigated the extent of P. aeruginosa transmission in New Zealand CF clinics.New Zealand's seven major CF centres were assessed, combining epidemiological data with computer-assisted SalI DNA fingerprinting of 496 isolates from 102 patients.One cluster of related isolates was significantly more prevalent in the largest clinic than expected by chance. The seven patients with isolates belonging to this cluster had more contact with each other than the remaining patients attending this centre. No other convincing evidence of transmission was found in any of the other smaller clinics. Three P. aeruginosa strains believed to be transmissible between patients in Australian and British CF clinics are present in New Zealand, but there was no definite evidence they had spread.Pseudomonas aeruginosa transmission is currently infrequent in New Zealand cystic fibrosis clinics. This situation could change rapidly and ongoing surveillance is required. The current results confirm that computer-assisted SalI DNA fingerprinting is ideally suited for such surveillance.

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“…P. aeruginosa is widely distributed in the natural environment Aoi et al, 2000 , which increases the risk of it contaminating water dispensers. However, this bacterium is a pathogen associated with opportunistic infections Edamoto et al, 2009 , requiring special attention with respect to the potential for healthcare-associated infections Spencker et al, 2000 . Furthermore, Ralstonia pickettii Woo et al, 2002 and R. insidiosa Van der beek et al, 2005 , which exhibited the highest isolation rates observed in this study, also include species that may influence health. These bacteria are widely distributed in the natural world, including in water, soil and plants.…”

Section: Proposed Using a C E T I C A C I D O R H Y D R O G E N P E Rmentioning

confidence: 61%

Bacterial Contamination in Cold Water Samples Obtained from Water Dispensers

2015

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Epidemiologic characterization of Pseudomonas aeruginosa in patients with cystic fibrosis

Abstract: Typing with PFGE is well suited for detailed investigations of colonization with Pseudomonas aeruginosa in cystic fibrosis patients. Pyocin typing can provide additional information for epidemiologic purposes.

Cited by 44 publications

References 21 publications

Clonal strains of Pseudomonas aeruginosa in paediatric and adult cystic fibrosis units

Clonal strains of Pseudomonas aeruginosa in paediatric and adult cystic fibrosis units

Pseudomonas aeruginosa transmission is infrequent in New Zealand cystic fibrosis clinics

Bacterial Contamination in Cold Water Samples Obtained from Water Dispensers

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