Epidemiologia molecular de surto de raiva bovina na região central do Rio Grande do Sul, 2012

Kanitz, Fábio Adriano; Kowalski, Ananda Paula; Batista, Helena Beatriz de Carvalho Ruthner; Carnieli, Pedro; Oliveira, Rafael de Novaes; Weiblen, Rudi; Flores, Eduardo F.

doi:10.1590/s0103-84782014000500012

Cited by 5 publications

(5 citation statements)

References 9 publications

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“…Sublineage 1A was more widely distributed than 1B and was detected in all RS regions sampled. This sublineage clustered with RABV isolates from regions in northeast and central RS obtained in previous studies [16,24]; the same sublineage has also been detected in southeastern Brazil (in the states of São Paulo, Rio de Janeiro and Minas Gerais), northern Brazil (in the State of Pará), Argentina and Ecuador, evidencing its wide geographic distribution. Sublineage 1B, in contrast, seemed to have a more restricted geographic distribution, since it was detected only in the states of RS and SC, the two most southern states in Brazil, and in Argentina and Uruguay, countries bordering southern Brazil.…”

Section: Identification Of Sublineages Of Rabvsupporting

confidence: 80%

“…Consequently, new outbreaks of rabies in cattle can be expected to occur and are invariably associated with the presence of infected vampire bats in the region. However, more detailed studies aiming to investigate the genetic lineages of RABV that may be circulating in southern Brazil are scarce [16,[23][24][25]. In view of this scarcity, in the present study, the coding region sequences of the RABV N gene from isolates recovered from infected cattle in the states of RS and SC were sequenced and compared to equivalent sequences available in GenBank.…”

Section: Identification Of Sublineages Of Rabvmentioning

confidence: 99%

“…In view of this scarcity, in the present study, the coding region sequences of the RABV N gene from isolates recovered from infected cattle in the states of RS and SC were sequenced and compared to equivalent sequences available in GenBank. Such an approach has been used in a number of previous studies [7,8,21,23,24]. Through examination of the topology of the phylogenetic tree generated by the analyses, two genetic sublineages of RABV (1A and 1B) were identified to be circulating in southern Brazil, both having the vampire bat D. rotundus as their natural reservoir.…”

Section: Identification Of Sublineages Of Rabvmentioning

confidence: 99%

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Phylogenetic analysis of rabies viruses isolated from cattle in southern Brazil

Fernandes¹,

Carnieli²,

Gregório³

et al. 2020

Virus Genes

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Bats and dogs are the main reservoirs of rabies virus (RABV) in Latin America and are responsible for the maintenance of different cycles of infection. In the two neighbour and most southern Brazilian states of Rio Grande do Sul (RS) and Santa Catarina (SC), rabies in dogs has been successfully controlled for more than 30 years. However, rabies associated to the rural cycle remains endemic, with a significant, though oscillating-annual incidence of rabies in cattle. Despite the plethora of studies on genetic analyses of Brazilian RABV, isolates from southern Brazil have only scarcely been investigated. This work was performed to identify the genetic lineages of RABVs circulating in states of RS and SC. Fifty-nine RABV cattle isolates from RS and SC were selected and submitted to reverse transcription/polymerase chain reaction (RT-PCR) followed by sequencing of the nucleoprotein gene. In RS, the circulation of two sublineages (1A and 1B) of RABV was detected, both with characteristics of lineages usually detected in vampire bats (Desmodus rotundus). In SC, only one sublineage of RABV (1B) was detected. Nevertheless, the findings reported here are expected to contribute to the understanding of the biology of the virus in the region and its interactions with the natural host D. rotundus. Keywords Rural rabies • Genetic lineages • Rio Grande do Sul • Santa Catarina • BrazilEdited by Zhen F. Fu.

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Section: Identification Of Sublineages Of Rabvsupporting

confidence: 80%

Section: Identification Of Sublineages Of Rabvmentioning

confidence: 99%

Section: Identification Of Sublineages Of Rabvmentioning

confidence: 99%

See 1 more Smart Citation

Phylogenetic analysis of rabies viruses isolated from cattle in southern Brazil

Fernandes¹,

Carnieli²,

Gregório³

et al. 2020

Virus Genes

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“…This disease is associated with the presence of the virus in the aerial cycle, the maintenance of Chiroptera in the nature and human economic activities. Therefore, such factors predispose rabies propagation in these species; the livestock growth increases the offer of food and artificial shelters, which facilitate the maintenance and perpetuation of populations of bats Desmodus rotundus, besides ecological changes, which favor Chiroptera migration in the search for new sources of food (Brasil, 2009;Kanitz et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

“…Advancements have been obtained with control programs; however, rabies remains endemic in the Americas due to the presence of wild and domestic reservoirs (Wada et al, 2011). In the rural cycle of this disease, the vampire bat (Desmodus rotundus) is considered an important transmitter of the rabies virus (Kanitz et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Results of laboratory diagnosis of rabies in herbivores (cattle and horses): a retrospective study

Barrosa¹,

Langoni

Victória³

et al. 2018

Braz. J. Vet. Med.

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Rabies is a zoonosis of extreme importance to public health. Specifically in herbivores it causes economic losses, considering its lethality. The diagnosis provides information to describe the epidemiology of the disease in the regional study. For this purpose, a survey of Central Nervous System (CNS) materials, received for diagnosis in the Center-West region of the State of São Paulo, by the Zoonosis Diagnostic Service (SDZ), Faculdade de Medicina Veterinária e Zootecnia (FMVZ)-UNESP-Botucatu-SP. In the period from 2001 to 2015, a total of 643 samples were analyzed, being 489 (76.05%) of the bovine species, with 68 (13.9%) positive samples and 154 (23.95%) of the equine species with 17 (11.3%) positive. For the analyzed bovine samples, sensitivity and specificity values of the fluorescent antibody test (FAT) were 88.24 and 99.52% respectively and for equines, 47.06 and 100.0%. The frequency of samples received by species, month, and year and the results of the diagnostic tests recommended by the World Health Organization (WHO), fluorescent antibody test (FAT) and the mouse inoculation test (MIT), were analysed.

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Glycoprotein-G-gene-based molecular and phylogenetic analysis of rabies viruses associated with a large outbreak of bovine rabies in southern Brazil

et al. 2017

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A large outbreak of hematophagous-bat-associated bovine rabies has been occurring in Rio Grande do Sul (RS), the southernmost Brazilian state, since 2011, with official estimates exceeding 50,000 cattle deaths. The present article describes a genetic characterization of rabies virus (RABV) recovered from 59 affected cattle and two sheep, from 56 herds in 16 municipalities (2012-2016). Molecular analysis was performed using the nucleotide (nt) and predicted amino acid (aa) sequences of RABV glycoprotein G (G). A high level of nt and aa sequence identity was observed among the examined G sequences, ranging from 98.4 to 100%, and from 97.3 to 100%, respectively. Likewise, high levels of nt and aa sequence identity were observed with bovine (nt, 99.8%; aa, 99.8%) and hematophagous bat (nt, 99.5%; aa, 99.4%) RABV sequences from GenBank, and lower levels were observed with carnivore RABV sequences (nt, 92.8%; aa, 88.1%). Some random mutations were observed in the analyzed sequences, and a few consistent mutations were observed in some sequences belonging to cluster 2, subcluster 2b. The clustering of the sequences was observed in a phylogenetic tree, where two distinct clusters were evident. Cluster 1 comprised RABV sequences covering the entire study period (2012 to 2016), but subclusters corresponding to different years could be identified, indicating virus evolution and/or introduction of new viruses into the population. In some cases, viruses from the same location obtained within a short period grouped into different subclusters, suggesting co-circulation of viruses of different origins. Subcluster segregation was also observed in sequences obtained in the same region during different periods, indicating the involvement of different viruses in the cases at different times. In summary, our results indicate that the outbreaks occurring in RS (2012 to 2016) probably involved RABV of different origins, in addition to a possible evolution of RABV isolates within this period.

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Epidemiologia molecular de surto de raiva bovina na região central do Rio Grande do Sul, 2012

Cited by 5 publications

References 9 publications

Phylogenetic analysis of rabies viruses isolated from cattle in southern Brazil

Phylogenetic analysis of rabies viruses isolated from cattle in southern Brazil

Results of laboratory diagnosis of rabies in herbivores (cattle and horses): a retrospective study

Glycoprotein-G-gene-based molecular and phylogenetic analysis of rabies viruses associated with a large outbreak of bovine rabies in southern Brazil

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