Epidemics of Dermatitis

Baghchechi, Mohsen; Goldenberg, Alina; Jacob, Sharon E.

doi:10.1016/j.iac.2021.04.005

Cited by 1 publication

(2 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Metal allergies are the most common cause of allergic contact dermatitis and may underlie implant failure 1‐3 . This public health concern is dominated by nickel allergy, affecting ~11% of the general population in Europe and North America 4 .…”

Section: Introductionmentioning

confidence: 99%

“…Metal allergies are the most common cause of allergic contact dermatitis and may underlie implant failure. [1][2][3] This public health concern is dominated by nickel allergy, affecting ~11% of the general population in Europe and North America. 4 Co-sensitization to cobalt and palladium, adjacent elements in the periodic table of the elements, is frequent but the mechanisms of antigen recognition and the extent of immunological cross-reactivity remain unclear.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Unique and common TCR repertoire features of Ni²⁺‐, Co²⁺‐, and Pd²⁺‐specific human CD154 + CD4+ T cells

Riedel

Aparicio‐Soto

Curato

et al. 2022

Allergy

View full text Add to dashboard Cite

Background: Apart from Ni 2+ , Co 2+ , and Pd 2+ ions commonly trigger T cell-mediated allergic contact dermatitis. However, in vitro frequencies of metal-specific T cells and the mechanisms of antigen recognition remain unclear.Methods: Here, we utilized a CD154 upregulation assay to quantify Ni 2+ -, Co 2+ -, and Pd 2+ -specific CD4+ T cells in peripheral blood mononuclear cells (PBMC). Involved αβ T cell receptor (TCR) repertoires were analyzed by high-throughput sequencing.Results: Peripheral blood mononuclear cells incubation with NiSO 4 , CoCl 2 , and PdCl 2 increased frequencies of CD154 + CD4+ memory T cells that peaked at ~400 μM.Activation was TCR-mediated as shown by the metal-specific restimulation of T cell clones. Most abundant were Pd 2+ -specific T cells (mean 3.5%, n = 19), followed by Co 2+ -and Ni 2+ -specific cells (0.6%, n = 18 and 0.3%, n = 20) in both allergic and nonallergic individuals. A strong overrepresentation of the gene segment TRAV9-2 was unique for Ni 2+ -specific TCR (28% of TCR) while Co 2+ and Pd 2+ -specific TCR favorably expressed TRAV2 (8%) and the TRBV4 gene segment family (21%), respectively. As a second, independent mechanism of metal ion recognition, all analyzed metal-specific TCR showed a common overrepresentation of a histidine in the complementarity determining region 3 (CDR3; 15% of α-chains, 34% of β-chains). The positions of the CDR3 histidine among metal-specific TCR mirrored those in random repertoires and were conserved among cross-reactive clonotypes.Conclusions: Induced CD154 expression allows a fast and comprehensive detection of Ni 2+ -, Co 2+ -, and Pd 2+ -specific CD4+ T cells. Distinct TCR repertoire features underlie the frequent activation and cross-reactivity of human metal-specific T cells.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Unique and common TCR repertoire features of Ni²⁺‐, Co²⁺‐, and Pd²⁺‐specific human CD154 + CD4+ T cells

Riedel

Aparicio‐Soto

Curato

et al. 2022

Allergy

View full text Add to dashboard Cite

show abstract

Epidemics of Dermatitis

Cited by 1 publication

References 80 publications

Unique and common TCR repertoire features of Ni²⁺‐, Co²⁺‐, and Pd²⁺‐specific human CD154 + CD4+ T cells

Unique and common TCR repertoire features of Ni²⁺‐, Co²⁺‐, and Pd²⁺‐specific human CD154 + CD4+ T cells

Contact Info

Product

Resources

About

Epidemics of Dermatitis

Cited by 1 publication

References 80 publications

Unique and common TCR repertoire features of Ni2+‐, Co2+‐, and Pd2+‐specific human CD154 + CD4+ T cells

Unique and common TCR repertoire features of Ni2+‐, Co2+‐, and Pd2+‐specific human CD154 + CD4+ T cells

Contact Info

Product

Resources

About

Unique and common TCR repertoire features of Ni²⁺‐, Co²⁺‐, and Pd²⁺‐specific human CD154 + CD4+ T cells

Unique and common TCR repertoire features of Ni²⁺‐, Co²⁺‐, and Pd²⁺‐specific human CD154 + CD4+ T cells