Epidemic spread of OXA-48 beta-lactamase in Croatia

Abstract

The OXA-48-positive organisms found in this study showed wide variability in antibiotic susceptibility, β-lactamase content and PFGE banding patterns. This study revealed a switch from the predominance of VIM-1 in 2012-2013 to that of OXA-48 in the 2015 to 2017.

Hidden Carbapenem Resistance in OXA-48 and Extended-Spectrum β-Lactamase-PositiveEscherichia coli

“…36,37 In the previous studies in Croatia K. pneumoniae was the first and the dominant species harboring OXA-48. [13][14][15] Similarly, as in previous studies, there was the polyclonal dissemination of OXA-48-positive E. coli and horizontal transmission of L plasmid determinant in E. coli. In contrast to the previous study, OXA-48 was the sole carbapenemase in the reported isolates.…”

“…28,33,34 The previous identification of OXA-48 b-lactamase in K. pneumoniae and E. cloacae raises the possibility of intergeneric conjugative gene transfer between different species of Enterobacteriaceae. [13][14][15] All except one isolate were collected from patients located at surgical departments and/or ICU and belonged to two clusters, as confirmed by PFGE. Long-term hospitalization, surgical procedures, and antimicrobial pressure have probably led to the selection of such isolates.…”

“…14 In the recent multicenter study on carbapenemases in Croatia, three K. pneumoniae and one E. cloacae isolate from Slavonski Brod located in Slavonia region of Croatia were shown to possess OXA-48. 15 During 2016, 102 ESBL-producing E. coli from primarily sterile specimens and other samples, such as urine, wounds, abdominal drainage, surveillance cultures, were collected from hospitalized and community patients in northeast part of Croatia, in Slavonia region. The resistance phenotype of these isolates implied a CTX-M-type b-lactamase with high level of resistance to cefotaxime and ceftriaxone.…”

Genomic Characterization of New Variant of Hydrogen Sulfide (H2S)-Producing Escherichia coli with Multidrug Resistance Properties Carrying the mcr-1 Gene in China

“…Therefore, the presence of a rare sulfonamides resistance gene, sul3, could be an interesting characteristic of this H 2 S-producing E. coli strain. Antibiotic resistance genes found in this study were also reported previously in E. coli isolated from humans in various studies in Australia [19], Argentina [20], Tunisia [21], Croatia [22], Sweden [23], Spain [24], Bolivia and Peru [25], Algeria [26], Nigeria [27], and Lithuania [28].…”

OXA-48 Carbapenemase in Klebsiella pneumoniae Sequence Type 307 in Ecuador

“…We recently reported a strain of Raoutella ornithinolytica that harbored the blaOXA-48-like gene with no plasmid characterization that was recovered after this strain [59], and to the best of our knowledge, this is the first description of carrying OXA-48-like carbapenemase in an IncL plasmid isolated in Ecuador. Interestingly, our patient came from Croatia were this mechanism of resistance was reported previously [60][61][62], indicating that contagion with this strain most likely happened in Europe. The implications of globalization and free travel are now being revealed with the fast spread of the SARS-CoV-19, but in reality, this is nothing new and it has long been the case with mechanisms of resistance.…”