Epidemic of influenza A(H1N1)pdm09 analyzed by full genome sequences and the first case of oseltamivir-resistant strain in Myanmar 2017

Win, Su Mon; Saito, Reiko; Win, Nay; Lasham, Di Ja; Kyaw, Yadanar; Lin, Nay; Thein, Khin Nyo; Chon, Irina; Odagiri, Takashi; Thein, Win; Kyaw, Latt Latt; Tin, Ommar Swe; Saitoh, Akihiko; Tamura, Tsutomu; Hirokawa, Chika; Uchida, Yuko; Saito, Takehiko; Watanabe, Shinji; Odagiri, Takato; Kamata, Kazuhiro; Osada, Hiroyuki; Dapat, Clyde; Watanabe, Hirotaka; Tin, Htay Htay

doi:10.1371/journal.pone.0229601

Cited by 18 publications

(9 citation statements)

References 30 publications

(46 reference statements)

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“…The mixture was incubated at 46 °C, and the fluorescence was monitored. RT-PCR and sequencing were performed as a gold standard at Tosoh Corporation based on the Influenza Diagnosis Manual [ 8 – 10 ]. Total RNA was isolated from 140 μL of TRC extraction buffer mixed with a nasopharyngeal swab or 140 μL of a gargle specimen using the Qiagen RNeasy kit.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples

Kaku

Hashiguchi

Akamatsu

et al. 2021

Eur J Clin Microbiol Infect Dis

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We evaluated a novel transcription-reverse transcription concerted reaction (TRC) assay that can detect influenza A and B within 15 min using nasopharyngeal swab and gargle samples obtained from patients with influenza-like illness, between January and March 2018 and between January and March 2019. Based on the combined RT-PCR and sequencing results, in the nasal swabs, the sensitivity and specificity of TRC for detecting influenza were calculated as 1.000 and 1.000, respectively. In the gargle samples, the sensitivity and specificity of TRC were 0.946 and 1.000, respectively. The TRC assay showed comparable performance to RT-PCR in the detection of influenza viruses.

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Section: Methodsmentioning

confidence: 99%

Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples

Kaku

Hashiguchi

Akamatsu

et al. 2021

Eur J Clin Microbiol Infect Dis

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“…The peak of ALRI in the current study occurred from August to October, which coincides with the second half of the rainy season. In contrast, the influenza season occurs in the first half of the rainy season which is in July or August [ 28 , 50 ].…”

Section: Discussionmentioning

confidence: 99%

Clinical manifestations and outcome of viral acute lower respiratory infection in hospitalised children in Myanmar

Kamata

Thein

et al. 2022

BMC Infect Dis

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Background Acute lower respiratory infection (ALRI) remains the leading cause of death in children worldwide, and viruses have been the major cause of ALRI. In Myanmar, ALRI is associated with high morbidity and mortality in children, and detailed information on ALRI is currently lacking. Methods This prospective study investigated the viral aetiologies, clinical manifestations, and outcomes of ALRI in hospitalised children aged 1 month to 12 years at the Yankin Children Hospital, Yangon, Myanmar from May 2017 to April 2019. The sample size was set to 300 patients for each year. Two nasopharyngeal swabs were obtained for the patients with suspected viral ALRI; one for rapid tests for influenza and respiratory syncytial virus (RSV), and the other for real-time PCR for the 16 ALRI-causing viruses. Pneumococcal colonization rates were also investigated using real-time PCR. Clinical information was extracted from the medical records, and enrolled patients were categorised by age and severity for comparison. Results Among the 5463 patients admitted with a diagnosis of ALRI, 570 (10.4%) were enrolled in this study. The median age of the patients was 8 months (interquartile range, 4–15 months). The most common symptoms were cough (93%) and difficulty in breathing (73%), while the most common signs of ALRI were tachypnoea (78%) and chest indrawing (67%). A total of 16 viruses were detected in 502 of 570 patients’ samples (88%), with RSV B (36%) and rhinovirus (28%) being the most commonly detected. Multiple viruses were detected in 221 of 570 samples (37%) collected from 570 patients. Severe ALRI was diagnosed in 107 of 570 patients (19%), and RSV B and human rhinovirus were commonly detected. The mortality rate was 5%; influenza virus A (29%) and RSV B (21%) were commonly detected, and stunting and lack of immunization were frequently observed in such cases. Additionally, 45% (259/570) of the patients had pneumococcal colonization. Conclusions Viral ALRI in hospitalised children with a median of 8 months has significant morbidity and mortality rates in Myanmar. RSV and rhinovirus were the most commonly detected from nasopharyngeal swabs, while influenza virus and RSV were the most frequently associated with fatal cases.

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“…Few US FDA-licensed anti-influenza A virus drugs are available for clinical despite use, massive screening for new agents against IAV. The NA inhibitor oseltamivir is the main agent for use against IAV, but pathogen mutations have conferred resistance to oseltamivir in influenza A(H7N9) viruses 36 , 37 . Thus, NA inhibitors have limited efficacy against seasonal influenza viruses and emphasize the need to develop alternative anti-IAV therapies.…”

Section: Discussionmentioning

confidence: 99%

Potent sialic acid inhibitors that target influenza A virus hemagglutinin

Chang

Yeh

Cheng

et al. 2021

Sci Rep

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Eradicating influenza A virus (IAV) is difficult, due to its genetic drift and reassortment ability. As the infectious cycle is initiated by the influenza glycoprotein, hemagglutinin (HA), which mediates the binding of virions to terminal sialic acids moieties, HA is a tempting target of anti-influenza inhibitors. However, the complexity of the HA structure has prevented delineation of the structural characterization of the HA protein–ligand complex. Our computational strategy efficiently analyzed > 200,000 records of compounds held in the United States National Cancer Institute (NCI) database and identified potential HA inhibitors, by modeling the sialic acid (SA) receptor binding site (RBS) for the HA structure. Our modeling revealed that compound NSC85561 showed significant antiviral activity against the IAV H1N1 strain with EC50 values ranging from 2.31 to 2.53 µM and negligible cytotoxicity (CC50 > 700 µM). Using the NSC85561 compound as the template to generate 12 derivatives, robust bioassay results revealed the strongest antiviral efficacies with NSC47715 and NSC7223. Virtual screening clearly identified three SA receptor binding site inhibitors that were successfully validated in experimental data. Thus, our computational strategy has identified SA receptor binding site inhibitors against HA that show IAV-associated antiviral activity.

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Epidemic of influenza A(H1N1)pdm09 analyzed by full genome sequences and the first case of oseltamivir-resistant strain in Myanmar 2017

Cited by 18 publications

References 30 publications

Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples

Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples

Clinical manifestations and outcome of viral acute lower respiratory infection in hospitalised children in Myanmar

Potent sialic acid inhibitors that target influenza A virus hemagglutinin

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