Epicoccum purpurascens for biocontrol of Sclerotinia head rot of sunflower

Pieckenstain, Fernando Luis; Bazzalo, M. E.; Roberts, A. M. I.; Ugalde, Rodolfo A.

doi:10.1017/s0953756200003129

Cited by 43 publications

(24 citation statements)

References 17 publications

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“…The fungus was routinely maintained on Czapek-Dox slants at 4 ° C and ascospores were obtained as described elsewhere (Pieckenstain et al ., 2001a). Nicotiana tabacum L. cv Wisconsin 38 plants were grown at 22 ° C with a 16-h photoperiod.…”

Section: Biological Materialsmentioning

confidence: 99%

“…Final volume of the incubation medium was 40 µl per well and plates were incubated for 18 h at 24 ° C. Percentage of ascospore germination was determined by observation under a binocular microscope, as described in a previous report (Pieckenstain et al ., 2001a). When necessary, germ-tube lengths were also determined.…”

Section: Effect Of Inhibitors On Ascospore Germination and Mycelial Gmentioning

confidence: 99%

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Polyamine metabolism during the germination of Sclerotinia sclerotiorum ascospores and its relation with host infection

et al. 2004

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Summary• Polyamine biosynthesis inhibitors were used to study polyamine metabolism during the germination of Sclerotinia sclerotiorum ascospores, and to evaluate the potential of polyamine biosynthesis inhibition for the control of ascospore-borne diseases in plants.• The effects of inhibitors on ascospore germination, free polyamine levels, ornithine decarboxylase activity and development of disease symptoms on tobacco ( Nicotiana tabacum ) leaf discs inoculated with ascospores were determined.• α -Difluoromethylornithine inhibited ornithine decarboxylase and decreased free spermidine levels, but had no effect on ascospore germination. Both, the spermidine synthase inhibitor cyclohexylamine and the S -adenosyl-methionine decarboxylase inhibitor methylglyoxal bis -[guanyl hydrazone] decreased free spermidine levels, but only the latter inhibited ascospore germination, at concentrations of 5 m M or higher. Lesion development on leaf discs was reduced by cyclohexylamine and methylglyoxal bis -[guanyl hydrazone], but not by α -difluoromethylornithine. In the absence of inhibitors, dormant ascospores contained higher polyamine levels than mycelium.• Ascospore germination did not depend on ornithine decarboxylase activity and inhibitors of this enzyme will probably have a limited potential for the control of ascospore-borne plant diseases. On the contrary, spermidine synthase and S -adenosylmethionine decarboxylase could be more suitable targets for fungicidal action. The relative insensitivity of ascospore germination to polyamine biosynthesis inhibitors may be caused by their high polyamine content.

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Section: Biological Materialsmentioning

confidence: 99%

Section: Effect Of Inhibitors On Ascospore Germination and Mycelial Gmentioning

confidence: 99%

Polyamine metabolism during the germination of Sclerotinia sclerotiorum ascospores and its relation with host infection

et al. 2004

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“…Biocontrol agents may seemingly represent an environmentally friendly alternative to potent and toxic fungicides, which cannot be broken down in the environment (Abdalla et al 2014). There are many studies on the biological control of S. sclerotiorum by using fungal (Krutova 1987;Hannusch & Boland 1995;Mischke et al 1995;McLaren et al 1996;Huang et al 2000;Pieckenstain et al 2001;Zhang et al 2004;Huang & Erickson 2007) and bacterial (Godoy et al 1990;Yuen et al 1991Yuen et al , 1994Expert & Digat 1995;Boland 1997;Tozlu 2003;Fernando et al 2004;Abdullah et al 2008) biocontrol agents in either laboratory or field-trials. However, many field studies failed to consistently control this pathogen due to the fact that Sclerotinia ascospores can disperse for a long distance and then even a reduced number of sclerotia in field can cause significant infection and yield loss (Venette 1998;Hedke et al 1999).…”

mentioning

confidence: 99%

Biological control of Sclerotinia sclerotiorum (Lib.) de Bary, the causal agent of white mould disease in red cabbage, by some bacteria

Tozlu¹,

Mohammadi²,

Kotan³

et al. 2016

Plant Prot. Sci.

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Tozlu E., Mohammadi P., Senol Kotan M., Nadaroglu H., Kotan R. (2016): Biological control of Sclerotinia sclerotiorum (Lib.) de Bary, the causal agent of white mould disease in red cabbage, by some bacteria. Plant Protect. Sci.,.Sclerotinia sclerotiorum (Lib.) de Bary is the causal agent of white mould, stem, and fruit rot diseases on a wide variety of crop plants including cabbage (Brassica oleracea L.) in field and storage. Control of this pathogen by using commercial disease management methods is extremely difficult. Therefore, this study was performed to develop an alternative and effective control method for the diseases by using biocontrol bacteria -Bacillus subtilis (strains TV-6F, TV-17C, TV-12H, BA-140 and EK-7), Bacillus megaterium (strains TV-103B), and Bacillus pumilus (strains RK-103) on Petri plate assays and on red cabbage in pot assays. On Petri plates, all of the tested bacterial strains showed the zone of inhibition against the pathogen fungus ranging 15.00-26.50 mm. Their percentage inhibition rates and lesion length ranged 42.64-79.41% and 0.02-4.50 cm in pot assays, respectively. Consequently, our results indicated that especially B. subtilis strains TV-17C, TV-12H, and TV-6F can be used as bio control agent of S. sclerotiorum in red cabbage production.

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“…Pojedini izolati E. nigrum proizvode niz sekundarnih metabolita koji se primenjuju za biološku kontrolu Monilinia spp. na breskvi i nektarini (Larena et al 2005, Mari et al 2007, Sclerotinia sclerotiorum na suncokretu (Pieckenstain et al 2001) i Pythium sp. na pamuku (Hashem & Ali 2004).…”

Section: Uvodunclassified

Epicoccum nigrum the new pathogen of sorghum seed in Serbia

Ristić¹,

Stanković²,

Vučurović³

et al. 2012

Ratar i povrt

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U periodu [2009][2010][2011]. godine na lokalitetima Bački Petrovac i Čantavir prikupljeno je i analizirano 16 uzoraka zaraženog semena gajenog sirka (Sorghum bicolor (L.) Moench.) sorti Alba, Gold, Prima i Reform na prisustvo fitopatogenih gljiva. U pojedinačnim i mešanim zarazama ustanovljeno je prisustvo vrsta iz rodova Epicoccum, Fusarium, Alternaria, Aspergillus i Penicillium. Iz zaraženog semena izolovane su monosporne kulture i na osnovu morfoloških osobina identifikovane su kao Epicoccum nigrum. Patogenost izolata ove gljive potvrđena je pojavom simptoma na veštački inokulisanim sejancima sirka. Molekularna identifikacija obavljena je primenom PCR i amplifikacije ITS regiona ribozomalne DNK. Sekvence gena odabranih izolata 291-09 ( JQ619838) i 315-09 ( JQ619839) pokazale su 99-100% nukleotidne identičnosti sa sekvencama 31 izolata E. nigrum deponovanih u GenBank bazi podataka. Dobijeni rezultati predstavljaju prvu detaljnu karakterizaciju E. nigrum u Srbiji. Prisustvo većeg broja vrsta gljiva na semenu sirka zahteva dalja ispitivanja njihovih međusobnih odnosa i značaja.

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Epicoccum purpurascens for biocontrol of Sclerotinia head rot of sunflower

Cited by 43 publications

References 17 publications

Polyamine metabolism during the germination of Sclerotinia sclerotiorum ascospores and its relation with host infection

Polyamine metabolism during the germination of Sclerotinia sclerotiorum ascospores and its relation with host infection

Biological control of Sclerotinia sclerotiorum (Lib.) de Bary, the causal agent of white mould disease in red cabbage, by some bacteria

Epicoccum nigrum the new pathogen of sorghum seed in Serbia

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