Epicardium-derived cells enhance proliferation, cellular maturation and alignment of cardiomyocytes

Weeke-Klimp, Alida H.; Bax, Noortje Noortje; Bellu, Anna Rita; Winter, Elizabeth M.; Vrolijk, J.; Plantinga, J.A.; Maas, Saskia; Brinker, Marja G. L.; Mahtab, Edris A F; Groot, Adriana C. Gittenberger‐de; Luyn, Marja J. A. van; Harmsen, Martin C.; Lie-Venema, Heleen

doi:10.1016/j.yjmcc.2010.07.007

Cited by 70 publications

(60 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Importantly, in the mouse, Wt1-lineage tracing after MI revealed that labelled cells remain on the heart surface (Zhou et al, 2011a). In vitro results suggest that coculture of cardiomyocytes with EPDCs enhances cardiomyocyte proliferation and promotes an efficient myofibre arrangement (Eid et al, 1992;Weeke-Klimp et al, 2010). In our experiments, wt1b:GFP cells were highly migratory.…”

Section: Epdcs Differentiate Into Myofibroblasts and Perivascular Fibmentioning

confidence: 46%

Pan-epicardial lineage tracing reveals that epicardium derived cells give rise to myofibroblasts and perivascular cells during zebrafish heart regeneration

González‐Rosa

Peralta

Mercader

2012

Developmental Biology

129

144

View full text Add to dashboard Cite

Myocardial infarction (MI) leads to a severe loss of cardiomyocytes, which in mammals are replaced by scar tissue. Epicardial derived cells (EPDCs) have been reported to differentiate into cardiomyocytes during development, and proposed to have cardiomyogenic potential in the adult heart. However, mouse MI models reveal little if any contribution of EPDCs to myocardium. In contrast to adult mammals, teleosts possess a high myocardial regenerative capacity. To test if this advantage relates to the properties of their epicardium, we studied the fate of EPDCs in cryoinjured zebrafish hearts. To avoid the limitations of genetic labelling, which might trace only a subpopulation of EPDCs, we used cell transplantation to track all EPDCs during regeneration. EPDCs migrated to the injured myocardium, where they differentiated into myofibroblasts and perivascular fibroblasts. However, we did not detect any differentiation of EPDCs nor any other non-cardiomyocyte population into cardiomyocytes, even in a context of impaired cardiomyocyte proliferation. Our results support a model in which the epicardium promotes myocardial regeneration by forming a cellular scaffold, and suggests that it might induce cardiomyocyte proliferation and contribute to neoangiogenesis in a paracrine manner.

show abstract

Section: Epdcs Differentiate Into Myofibroblasts and Perivascular Fibmentioning

confidence: 46%

Pan-epicardial lineage tracing reveals that epicardium derived cells give rise to myofibroblasts and perivascular cells during zebrafish heart regeneration

González‐Rosa

Peralta

Mercader

2012

Developmental Biology

129

144

View full text Add to dashboard Cite

show abstract

“…It cannot be excluded that inhibition of the outgrowth of the epicardium has secondary effects on the heart which can affect cardiac functioning, since epicardial cells are important for differentiation and maturation of cardiomyocytes [44]. However, our results show that epicardial covering is important for the response to epinephrine, since normal hearts without epicardium (HH15) do not respond to epinephrine.…”

Section: Discussionmentioning

confidence: 57%

The epicardium as modulator of the cardiac autonomic response during early development

Kelder

Duim

Vicente‐Steijn

et al. 2015

Journal of Molecular and Cellular Cardiology

Self Cite

View full text Add to dashboard Cite

“…The activated epicardium and EPDCs reexpressed WT1 not only in the MI border zone but also in remote areas. Another approach is represented by epicardial cells cocultured with cardiomyocytes in which EPDCs change myocardial alignment and contraction [23]. A direct approach was provided by grafting human adult atrial epicardial cells [24] cultured in vitro from a cobblestone epithelium into spindle shape, thereby acquiring characteristics of mesenchymal stem cells.…”

Section: Cardiovascular Repairmentioning

confidence: 99%

The Arterial Epicardium: A Developmental Approach to Cardiac Disease and Repair

Groot

Winter

Goumans

et al. 2016

Etiology and Morphogenesis of Congenital Heart Disease

Self Cite

View full text Add to dashboard Cite

The significance of the epicardium that covers the heart and the roots of the great arteries should not be underestimated as it is a major component with impact on development, disease, and repair. The epicardium differentiates from the proepicardial organ located at the venous pole (vPEO). The differentiation capacities of the vPEO into epicardium-derived cells (EPDCs) have been extensively described. A hitherto escaped part of the epicardium derives from a second proepicardial organ located at the arterial pole (aPEO) and covers the intrapericardial part of the aorta and pulmonary trunk. In avian and mouse embryos, disturbance of epicardium differentiation causes a spectrum of cardiac anomalies including coronary artery abnormalities, deficient annulus fibrosis with rhythm disturbances, valve malformations, and non-compaction cardiomyopathies. Late in prenatal life the epicardium becomes dormant, losing the activity of many genes.In human cardiac diseases, both arterial and venous epicardium can be activated again into EPDCs. The epicardial reactivation observed after experimental

show abstract

Epicardium-derived cells enhance proliferation, cellular maturation and alignment of cardiomyocytes

Cited by 70 publications

References 39 publications

Pan-epicardial lineage tracing reveals that epicardium derived cells give rise to myofibroblasts and perivascular cells during zebrafish heart regeneration

Pan-epicardial lineage tracing reveals that epicardium derived cells give rise to myofibroblasts and perivascular cells during zebrafish heart regeneration

The epicardium as modulator of the cardiac autonomic response during early development

The Arterial Epicardium: A Developmental Approach to Cardiac Disease and Repair

Contact Info

Product

Resources

About