Epiblast integrity requires CLASP and Dystroglycan-mediated microtubule anchoring to the basal cortex

Nakaya, Yukiko; Sukowati, Erike W.; Sheng, Guojun

doi:10.1083/jcb.201302075

Cited by 49 publications

(42 citation statements)

References 47 publications

(93 reference statements)

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“…Loss of dystroglycan did not perturb the F-actin ring. The F-actin ring might help localize dystroglycan, as dystroglycan can be stabilized at specific cellular domains by anchoring to the cytoskeleton (Nakaya et al, 2013). F-actin has also been implicated as possibly stabilizing an integrin receptor diffusion barrier during phagocytosis (Freeman et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Cell Invasion In Vivo via Rapid Exocytosis of a Transient Lysosome-Derived Membrane Domain

et al. 2017

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Summary Invasive cells use small invadopodia to breach basement membrane (BM), a dense matrix that encases tissues. Following the breach, a large protrusion forms to clear a path for tissue entry by poorly understood mechanisms. Using RNAi screening for defects in C. elegans anchor cell (AC) invasion, we found that UNC-6(netrin)/UNC-40(DCC) signaling at the BM breach site directs exocytosis of lysosomes using the exocyst and SNARE SNAP-29 to form a large protrusion that invades vulval tissue. Live-cell imaging revealed that the protrusion is enriched in the matrix metalloprotease ZMP-1 and transiently expands AC volume more than 20%, displacing surrounding BM and vulval epithelium. Photobleaching and genetic perturbations showed that the BM receptor dystroglycan forms a membrane diffusion barrier at the neck of the protrusion that enables protrusion growth. Together these studies define a netrin dependent pathway that builds an invasive protrusion, an isolated lysosome-derived membrane structure specialized to breach tissue barriers.

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Section: Discussionmentioning

confidence: 99%

Cell Invasion In Vivo via Rapid Exocytosis of a Transient Lysosome-Derived Membrane Domain

et al. 2017

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“…While several studies have implicated invadopodia with EMT, 75,117 direct visualization of their activity and formation is lacking. Moreover, there is evidence that cells cross basement membrane through a variety of mechanisms, including downregulation of basement membrane receptors, 118 physical forces that shift or breach the basement membrane, 79,83,119 entry through preformed portals, 66,67,120,121 and remodeling events that create absences or holes in the basement membrane. 62,[122][123][124] These studies will be important in evaluating whether targeting invadopodia would be an efficient approach to blocking cancer metastasis.…”

Section: Discussionmentioning

confidence: 99%

Invadopodia and basement membrane invasion in vivo

Lohmer

Kelley

Hagedorn

et al. 2014

Cell Adhesion & Migration

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“…However, no direct interaction occurs between LL5 and integrins, indicating that other adaptor proteins are also involved. In the pre-gastrulation epithelium of the epiblast, LL5 cooperates with CLASPs to maintain basement membrane integrity and anchors microtubules to the basal cell cortex (Nakaya et al, 2013). In these cells, CLASP1 and CLASP2 associate with a-dystroglycan, which is also a laminin receptor, raising the possibility that the ECM coordinates microtubular orientation by both dystroglycan and the laminin-binding integrins a3b1 and a6b1.…”

Section: Ilk Recruits Microtubules To Active B1 Integrin In Polarisedmentioning

confidence: 99%

Integrins and epithelial cell polarity

Lee

Streuli

2014

Journal of Cell Science

126

117

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Cell polarity is characterised by differences in structure, composition and function between at least two poles of a cell. In epithelial cells, these spatial differences allow for the formation of defined apical and basal membranes. It has been increasingly recognised that cell-matrix interactions and integrins play an essential role in creating epithelial cell polarity, although key gaps in our knowledge remain. This Commentary will discuss the mounting evidence for the role of integrins in polarising epithelial cells. We build a model in which both inside-out signals to polarise basement membrane assembly at the basal surface, and outside-in signals to control microtubule apical-basal orientation and vesicular trafficking are required for establishing and maintaining the orientation of epithelial cell polarity. Finally, we discuss the relevance of the basal integrin polarity axis to cancer.

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Epiblast integrity requires CLASP and Dystroglycan-mediated microtubule anchoring to the basal cortex

Abstract: Disruption of the CLASP- and Dystroglycan-mediated cortical microtubule anchoring reduces epiblast–basement membrane interactions and initiates gastrulation.

Cited by 49 publications

References 47 publications

Cell Invasion In Vivo via Rapid Exocytosis of a Transient Lysosome-Derived Membrane Domain

Cell Invasion In Vivo via Rapid Exocytosis of a Transient Lysosome-Derived Membrane Domain

Invadopodia and basement membrane invasion in vivo

Integrins and epithelial cell polarity

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