EphB2 promotes cervical cancer progression by inducing epithelial-mesenchymal transition

Gao, Qing; Liu, Wei; Cai, Jiangyi; Li, Mu; Gao, Yue; Lin, Wenjing; Li, Zong-Fang

doi:10.1016/j.humpath.2013.10.001

Cited by 47 publications

(50 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…26 Another important tyrosine kinase receptor is EphB2, the activation of which is recently demonstrated to promote EMT through Ras signal pathway in cervical cancer. 27 These tyrosine kinase receptors are usually highly activated to modulate the progression of cervical cancer. The EMT found in CSCC may be due to the coeffect of these molecules and AEG-1, although the association of AEG-1 with these molecules was rarely investigated in cervical cancer.…”

Section: Discussionmentioning

confidence: 99%

Astrocyte Elevated Gene-1 Promotes Progression of Cervical Squamous Cell Carcinoma by Inducing Epithelial-Mesenchymal Transition via Wnt Signaling

Song

Xiong

et al. 2015

International Journal of Gynecological Cancer

View full text Add to dashboard Cite

ObjectiveThe aim of this study was to investigate the association of astrocyte elevated gene-1 (AEG-1) with epithelial-mesenchymal transition of cervical squamous cell carcinoma (CSCC) and the underlying mechanisms.MethodsThe expression of proteins was determined by immunohistochemistry in tissues. Overexpression and knockdown of AEG-1 in SiHa cells were achieved by stable AEG-1 gene transfection (SiHa-AEG-1+) and AEG-1-siRNA (SiHa-AEG-1−), respectively. The cellular levels of messenger RNA and proteins were assessed with reverse transcription polymerase chain reaction and Western blotting, respectively. The cell invasion capacity was assessed by the chamber invasion assay.ResultsAEG-1 was overexpressed in clinical CSCC and associated with lymph node metastasis, parametrial involvement, stromal invasion, and vascular invasion. A high level of vimentin and a low level of E-cadherin were also detected in the cancer tissues. AEG-1 expression was positively correlated with vimentin expression and negatively with E-cadherin expression in CSCC tissues. In addition, high level of AEG-1 was related to unfavorable prognosis of CSCC. On a cellular level, overexpression of AEG-1 was found to lead to an up-regulation of vimentin and a down-regulation of E-cadherin on messenger RNA and protein level in SiHa cells, whereas AEG-1 knockdown led to a contrary result. Meanwhile, the nuclear levels of NF-κB p65 and β-catenin were also increased in SiHa-AEG-1+, whereas their nuclear levels were decreased in SiHa-AEG-1−. Inhibition of Wnt signaling significantly reduced vimentin level and enhanced E-cadherin level in SiHa-AEG+, but inhibition of NF-κB signaling did not. SiHa-AEG-1+ and SiHa-AEG− showed an enhanced and a decreased invasive capacity, respectively. The enhanced invasiveness of SiHa-AEG-1+ was weakened by inhibition of Wnt signaling.ConclusionsAEG-1 was associated with the progression of CSCC by promoting epithelial-mesenchymal transition via Wnt signaling pathway.

show abstract

Section: Discussionmentioning

confidence: 99%

Astrocyte Elevated Gene-1 Promotes Progression of Cervical Squamous Cell Carcinoma by Inducing Epithelial-Mesenchymal Transition via Wnt Signaling

Song

Xiong

et al. 2015

International Journal of Gynecological Cancer

View full text Add to dashboard Cite

show abstract

“…EphB2 is reportedly highly expressed in several human cancers, and its overexpression plays an important role in cancer metastasis [56,57]. Eph2 expression is high in cervical cancer cells, it activates the R-Ras pathway and acts as an EMT inducer by imparting changes in the phenotype of cervical epithelial cells to elongated mesenchymal cells, which enhances the cell migratory and invasive properties [58]. Cells that undergo EMT have been shown to acquire a stem cell phenotype [59].…”

Section: Receptor Tyrosine Kinase and Emt In Cervical Cancermentioning

confidence: 99%

“…Cells that undergo EMT have been shown to acquire a stem cell phenotype [59]. The ectopic overexpression of EphB2 induces tumour sphere-forming ability and tumourigenesis potential, while silencing EphB2 decreases the stem cell properties [58].…”

Section: Receptor Tyrosine Kinase and Emt In Cervical Cancermentioning

confidence: 99%

See 1 more Smart Citation

EMT in cervical cancer: Its role in tumour progression and response to therapy

2015

View full text Add to dashboard Cite

“…(77,81) The Eph receptors/ephrins have also been implicated in tumourigenesis, progression and metastasis across a wide range of malignancies (Table 3). ( (83) Prostate cancer (84,85) Breast cancer (86,87) Melanoma (88) Glioblastoma (89,90) Gastric cancer (91) EphA3 Gastric cancer (92) Colorectal cancer (93) EphB2 Advanced cSCC (94) Glioblastoma (95)(96)(97) Cervical cancer (98) Breast cancer (99) EphB4 HNSCC (100) Non-small cell lung cancer (101) Breast cancer (99) Downregulated EphA1 NMSC (77) …”

Section: Galaninmentioning

confidence: 99%

Molecular mechanisms of perineural spread of cutaneous carcinoma: a potential role for receptor tyrosine kinases and other biomarkers

Schmidt¹

View full text Add to dashboard Cite

Perineural invasion and spread of cutaneous squamous cell carcinoma of the head and neck (cSCCHN) is associated with worse prognosis and high rates of locoregional recurrence. It occurs in less than 5% of cases, but given the high incidence of cSCC in Australia, portends significant morbidity and mortality. Primary tumour biology remains poorly understood and precise molecular mechanisms by which malignant squamous cells invade and progress axially within the perineural space remain unclear. Thus, there is no targeted therapy for perineural spread of cSCCHN or other neurotropic malignancies. Dysregulation of cell membrane receptor trafficking is a hallmark of cancer and such receptors are potential therapeutic targets.Over-expression of the epidermal growth factor receptor (EGFR) is well described in squamous cell carcinoma and the monoclonal antibody cetuximab is approved for advanced mucosal head Ultimately, we aim to improve survival and quality of life for sufferers of this morbid form of tumour spread. Our findings may also have implications for other neurotropic malignancy, including pancreatic, gastric, colorectal and prostate cancers. 4 Declaration by authorThis thesis is composed of my original work, and contains no material previously published or written by another person except where due reference has been made in the text. I have clearly stated the contribution by others to jointly-authored works that I have included in my thesis.

show abstract

EphB2 promotes cervical cancer progression by inducing epithelial-mesenchymal transition

Cited by 47 publications

References 29 publications

Astrocyte Elevated Gene-1 Promotes Progression of Cervical Squamous Cell Carcinoma by Inducing Epithelial-Mesenchymal Transition via Wnt Signaling

Astrocyte Elevated Gene-1 Promotes Progression of Cervical Squamous Cell Carcinoma by Inducing Epithelial-Mesenchymal Transition via Wnt Signaling

EMT in cervical cancer: Its role in tumour progression and response to therapy

Molecular mechanisms of perineural spread of cutaneous carcinoma: a potential role for receptor tyrosine kinases and other biomarkers

Contact Info

Product

Resources

About