EpCAM-based assays for epithelial tumor cell detection in cerebrospinal fluid

Bussel, Mark T J van; Pluim, Dick; Bol, Mijke; Beijnen, Jos H.; Schellens, Jan H.M.; Brandsma, Dieta

doi:10.1007/s11060-017-2691-6

Cited by 28 publications

(25 citation statements)

References 34 publications

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“…In these assays, immunomagnetic enrichment with anti-EpCAM (or anti-MCSP) MicroBeads prior to flow cytometry is used. 17,19,28 To distinguish CTCs from leukocytes, anti-CD45 fluorescein isothiocyanate for leukocyte labeling is added. In addition to these markers, some groups use anti-CD33 to improve differentiation between monocyte/macrophages/granulocytes (CD45− CD33+ CD326+) and epithelial (tumor) cells (CD45− CD33− CD326+).…”

Section: Circulating Tumor Cellsmentioning

confidence: 99%

Liquid biopsy in central nervous system metastases: a RANO review and proposals for clinical applications

et al. 2019

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Liquid biopsies collect and analyze tumor components in body fluids, and there is an increasing interest in the investigation of liquid biopsies as a surrogate for tumor tissue in the management of both primary and secondary brain tumors. Herein we critically review available literature on spinal fluid and plasma circulating tumor cells (CTCs) and cell-free tumor (ctDNA) for diagnosis and monitoring of leptomeningeal and parenchymal brain metastases. We discuss technical issues and propose several potential applications of liquid biopsies in different clinical settings (ie, for initial diagnosis, for assessment during treatment, and for guidance of treatment decisions). Last, ongoing clinical studies on CNS metastases that include liquid biopsies are summarized, and recommendations for future clinical studies are provided.

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Section: Circulating Tumor Cellsmentioning

confidence: 99%

Liquid biopsy in central nervous system metastases: a RANO review and proposals for clinical applications

et al. 2019

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“…Flow cytometry (FC) is widely used in the diagnosis of hematological neoplasms, but despite having demonstrated its potential usefulness, FC is not routinely used in the diagnosis or follow-up of nonhematopoietic neoplasms (Acosta, Pereira, & Arroz, 2016;Davidson,2016;Dorwal, Moore, Stewart, Harrison, & Monaghan,2018;Pillai, Cibas, & Dorfman, 2013;Pillai & Dorfman, 2016;van Bussel et al, 2018). In concordance, the existing literature about this subject is scarce.…”

Section: Introductionmentioning

confidence: 99%

The utility of flow cytometry in the diagnostic work up of malignant effusions due to nonhematopoietic neoplasms

Wong-Arteta

Rey

Aragón

et al. 2020

Cytometry Part B Clinical

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Malignant pleural effusion and peritoneal carcinomatosis are frequent causes of effusion. Cytological evaluation (PAP-stained slides followed by immunocytochemistry, IHC, if applicable) is currently the gold standard for the diagnosis of malignant effusions, but its sensitivity varies between 40 and 80%, being a time-consuming technique. Although flow cytometry (FC) is not routinely used in the diagnosis or follow-up of nonhematopoietic neoplasms, it has the advantage of being rapidly applicable to fresh samples, potentially decreasing the time for the diagnosis. The main objective of this study was to assess the utility of FC as a confirmatory tool in the diagnosis of neoplastic effusions, based on the expression of EpCAM antibody in tumor cells versus the cytological evaluation. In this work 1,535 serous fluids were collected, of which 101 (68 pleural, 33 ascites) were selected through a screening algorithm and sent to the FC and cytological evaluation. Seventy-three of these samples (46 pleural, 27 ascites) were considered malignant as determined by clinical, cytological and radiological criteria. According to our data, 75% (55/73) of these samples were positive by Cytology/IHC and 74% (54/73) by FC. We noticed that, although the sensitivity, specificity, and area under the curve were similar, the turnaround time was shorter when using FC. Moreover, these results clearly improved by combining both techniques. We conclude that FC provides information about malignant effusions faster than immunohistochemical staining, and we believe that performing both techniques in parallel would improve diagnostic performance.

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“…Recent findings have indicated that a great deal of circRNAs might regulate miRNAs function as microRNA sponges and play a significant role in the regulation of transcription 21 , 22 . For instance, circPCNXL2 binds to miR-153 as a miRNA sponge to regulate ZEB2 expression in RCC progression 19 .…”

Section: Discussionmentioning

confidence: 99%

CircUBAP2 Inhibits Proliferation and Metastasis of Clear Cell Renal Cell Carcinoma via Targeting miR-148a-3p/FOXK2 Pathway

et al. 2020

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Clear cell renal cell carcinoma (ccRCC) is the prominent histological subtype of renal cell carcinoma (RCC) with high incidence of local recurrence and distant metastasis. It has been documented that circular ribonucleic acids (circRNAs) play crucial roles in the development of cancers; however, study on exploring the role of circRNAs in ccRCC still remains limited. In the present study, we aimed to evaluate the biological function of a novel circRNA UBAP2 (circUBAP2) in ccRCC and the underlying mechanism. Our results showed that circUBAP2 expression was significantly down-regulated in ccRCC tissues and cell lines. Overexpression of circUBAP2 significantly inhibited the proliferation, migration, and invasion of ccRCC cells. MiR-148a-3p was a target miRNA of circUBAP2 in ccRCC cells, and its expression levels in ccRCC tissues and cell lines were negatively correlated with circUBAP2 levels. Moreover, miR-148a-3p reversed the inhibitory effects of circUBAP2 on cell proliferation, migration, and invasion in ccRCC cells. Additionally, forkhead box K2 (FOXK2) was found to be a target gene of miR-148a-3p and regulated by miR-148a-3p in ccRCC cells. Furthermore, knockdown of FOXK2 reversed the inhibitory effects of miR-148a-3p inhibitor on ccRCC cells. In conclusion, these findings indicated that circUBAP2 functioned as a novel tumor suppressor in ccRCC through regulating the miR-148a-3p/FOXK2 axis. Therefore, circUBAP2 might serve as a potential therapeutic target for the treatment of ccRCC.

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EpCAM-based assays for epithelial tumor cell detection in cerebrospinal fluid

Cited by 28 publications

References 34 publications

Liquid biopsy in central nervous system metastases: a RANO review and proposals for clinical applications

Liquid biopsy in central nervous system metastases: a RANO review and proposals for clinical applications

The utility of flow cytometry in the diagnostic work up of malignant effusions due to nonhematopoietic neoplasms

CircUBAP2 Inhibits Proliferation and Metastasis of Clear Cell Renal Cell Carcinoma via Targeting miR-148a-3p/FOXK2 Pathway

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