EP-1806: Characterization of hypoxia in three rat prostate tumor sublines, using dynamic F-MISO PET and histology

Romano, Patrizio; Chen, Cheng; Glowa, Christin; Peschke, Peter; Pan, Leyun; Haberkorn, Uwe; Dimitrakopoulou-Strauß, Antonia; Karger, Christian P.

doi:10.1016/s0167-8140(15)31924-1

Cited by 2 publications

(2 citation statements)

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“…Thus, even if the measured parameters are corrected for the impact of the AIF, it may still include some uncertainty resulting from the fact that the measured rather than the actual AIF of the tumour has been used. The strong dependence of the TAC on the AIF may also explain the very different TAC shapes observed by Mena-Romano et al [31] in three different rat prostate tumour sublines.…”

Section: Parametric Sensitivity Study For the Fmiso Dynamicsmentioning

confidence: 83%

Simulation of hypoxia PET-tracer uptake in tumours: Dependence of clinical uptake-values on transport parameters and arterial input function

et al. 2020

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Poor radiotherapy outcome is in many cases related to hypoxia, due to the increased radioresistance of hypoxic tumour cells. Positron emission tomography may be used to non-invasively assess the oxygenation status of the tumour using hypoxia-specific radiotracers. Quantification and interpretation of these images remains challenging, since radiotracer binding and oxygen tension are not uniquely related. Computer simulation is a useful tool to improve the understanding of tracer dynamics and its relation to clinical uptake parameters currently used to quantify hypoxia. In this study, a model for simulating oxygen and radiotracer distribution in tumours was implemented to analyse the impact of physiological transport parameters and of the arterial input function (AIF) on: oxygenation histograms, time-activity curves, tracer binding and clinical uptake-values (tissue-to-blood ratio, TBR, and a composed hypoxia-perfusion metric, FHP). Results were obtained for parallel and orthogonal vessel architectures and for vascular fractions (VFs) of 1% and 3%. The most sensitive parameters were the AIF and the maximum binding rate (K max ). TBR allowed discriminating VF for different AIF, and FHP for different K max , but neither TBR nor FHP were unbiased in all cases. Biases may especially occur in the comparison of TBRor FHP-values between different tumours, where the relation between measured and actual AIF may vary. Thus, these parameters represent only surrogates rather than absolute measurements of hypoxia in tumours.

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Section: Parametric Sensitivity Study For the Fmiso Dynamicsmentioning

confidence: 83%

Simulation of hypoxia PET-tracer uptake in tumours: Dependence of clinical uptake-values on transport parameters and arterial input function

et al. 2020

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“…In vivo preclinical imaging studies have also demonstrated their value in the translation of clinically relevant endpoints [5,6]. Current areas of research include the determination of parameters with relevance for application in radiotherapy, such as tumor hypoxia [7,8], or the impact of different types of radiation (e.g. photons or protons) on tissue [9,10].…”

Section: Introductionmentioning

confidence: 99%

Multi-modality bedding platform for combined imaging and irradiation of mice

Müller

Schürer

Neubert

et al. 2020

Biomed. Phys. Eng. Express

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Preclinical imaging and irradiation yields valuable insights into clinically relevant research topics. While complementary imaging methods such as computed tomography (CT), magnetic resonance imaging (MRI), and positron emission tomography (PET) can be combined within single devices, this is technically demanding and cost-intensive. Similarly, bedding and setup solutions are often specific to certain devices and research questions. We present a bedding platform for mice that is compatible with various preclinical imaging modalities (combined PET/MRI, cone beam CT) and irradiation with photons and protons. It consists of a 3D-printed bedding unit (acrylonitrile butadiene styrene, ABS) holding the animal and features an inhalation anesthesia mask, jaw fixation, ear pins, and immobilization for the hind leg. It can be embedded on mounting adaptors for multi-modal imaging and into a transport box (polymethyl methacrylate, PMMA) for experiments outside dedicated animal facilities while maintaining the animal’s hygiene status. A vital support unit provides heating, inhalation anesthesia, and a respiration monitor. We dosimetrically evaluated used materials in order to assess their interaction with incident irradiation. Proof-of-concept multi-modal imaging protocols were used on phantoms and mice. The measured attenuation of the bedding unit for 40/60/80/200 kV X-rays was less than 3%. The measured stopping-power-ratio of ABS was 0.951, the combined water-equivalent thickness of bedding unit and transport box was 4.2 mm for proton energies of 150 MeV and 200 MeV. Proof-of-concept imaging showed no loss of image quality. Imaging data of individual mice from different imaging modalities could be aligned rigidly. The presented bed aims to provide a platform for experiments related to both multi-modal imaging and irradiation, thus offering the possibility for image-guided irradiation which relies on precise imaging and positioning. The usage as a self-contained, stand-alone unit outside dedicated animal facilities represents an advantage over setups designed for specific devices.

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EP-1806: Characterization of hypoxia in three rat prostate tumor sublines, using dynamic F-MISO PET and histology

Cited by 2 publications

References 0 publications

Simulation of hypoxia PET-tracer uptake in tumours: Dependence of clinical uptake-values on transport parameters and arterial input function

Simulation of hypoxia PET-tracer uptake in tumours: Dependence of clinical uptake-values on transport parameters and arterial input function

Multi-modality bedding platform for combined imaging and irradiation of mice

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