Eosinophilic leukaemia: morphological, cytochemical, and electron microscopic studies.

Presentey, B.; Jerushalmy, Z.; Mintz, Uri

doi:10.1136/jcp.32.3.261

Cited by 18 publications

(6 citation statements)

References 32 publications

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“…Specifically, both red and blue granules can be seen in the HL-60 eosinophils stained with Wright-Giemsa, the granules are chloroacetate esterase and PAS positive (eosinophilic granules from normal persons are negative for both), and crystailoids are rare (8). The persistence of chloroacetate esterase activity has previously been described in eosinophilic leukemia (23) and the presence of multilameilar inclusions and the paucity of crystailoids in secondary granules as seen through the electron microscope has also been described in eosinophils from lymphoma patients (19). Additionally, vacuolization of the cytoplasm has been described in circulating eosinophils in various pathologic states although normal eosinophilic precursors may vacuolate in the bone marrow (24), as may circulating normal eosinophils upon appropriate stimulation (25).…”

Section: Discussionmentioning

confidence: 79%

Eosinophilic differentiation of the human promyelocytic leukemia cell line, HL-60.

Fischkoff

Pollak

Gleich

et al. 1984

The Journal of Experimental Medicine

129

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By the addition of specific chemical agents to the culture medium, the HL-60 leukemia cell line, derived from a patient with acute promyelocytic leukemia (1), can be induced to mature to cells with many of the functional, morphological, and biochemical features of either neutrophils or macrophages (2, 3). Two recent studies strongly suggested that HL-60 cells also differentiated to eosinophils when cultured in soft agar (4, 5); the colonies from soft agar stained with Luxolfast-blue, a stain specific (among hematopoietic cells) for eosinophilic granules (6). Unfortunately, few cells differentiated to eosinophils, and it was technically difficult to harvest cells from semi-solid medium. These factors limit the usefulness of that system as a model for eosinophilopoiesis. Furthermore, the two reports disagreed on the role of added colony-stimulating factors. The recent description of increased numbers of morphologically abnormal eosinophils in the bone marrow of patients with FAB type M4 leukemia (AMMoL) 1 who have abnormalities of chromosome 16, del(16)(q22) (7) and inv(16)(p 13q22) (8), has further focused attention on the phenomenon of eosinophilic differentiation of leukemia cells. We observed that HL-60 cells cultured in slightly alkaline liquid medium develop granules typical in appearance to those found in eosinophils and eosinophilic precursors. Our higher induction efficiency and ease in harvesting cells from liquid culture permitted a more complete characterization of the differentiated cells. To determine whether HL-60 cells might serve as a model system for eosinophilopoiesis in general, or eosinophilic differentiation of leukemia cells in particular, the HL-60-derived eosinophils were characterized morphologically, histochemically, and cytogenetically.

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Section: Discussionmentioning

confidence: 79%

Eosinophilic differentiation of the human promyelocytic leukemia cell line, HL-60.

Fischkoff

Pollak

Gleich

et al. 1984

The Journal of Experimental Medicine

129

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“…Some of those cases may be considered variants of AML (M2, FAB) with predominant cosinophilic maturation. Ultrastructural studies in some cases (Ackerman, 1964;Weinger et al, 1975;Anteunis et al, 1978;Presentey et a / , 1979) appear to have shown marked morphological abnormalities in the eosinophils, evidence of eosinophilic differentiation and transitional forms between blasts and the more mature cells. We have not observed such transitional forms in the five cases studied by electron microscopy.…”

Section: Discussionmentioning

confidence: 99%

The Association of Eosinophilia with Lymphoblastic Leukaemia or Lymphoma: a Study of Seven Patients

Catovsky¹,

et al. 1980

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Seven patients with hypereosinophilia in association with a lymphoblastic malignancy are described. The eosinophilia preceded or was present at diagnosis in all patients. Eosinophil counts fell during complete remission but rose significantly before or during relapses in five patients. Hypogranular and sometimes Pelger-eosinophils were seen in five cases. Surface and enzyme markers defined the malignancy in six cases as common-ALL (three), T-ALL (two) and T-lymphoblastic lymphoma (one). Although a diagnosis of eosinophilic leukaemia or acute myeloid leukaemia with eosinophil differentiation was considered in three patients, cytochemical and ultrastructural studies failed to show any evidence of myeloid differentiation in the blast cells. The bone marrow karyotype was normal in the four patients studied. All seven patients had one or more relapses and six died 6-62 months from diagnosis. Severe complications of the hypereosinophilic syndrome developed in one patient. As T-lymphocytes have been shown to be involved in the induction of eosinophilia in rodents, it is suggested that the hypereosinophilia in these patients was induced by eosinopoietic stimuli produced by lymphoblasts.

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“…Naphthol AS-D chloroacetate esterase positivity similar to that seen here has been reported as a feature of eosinophilic leukaemia in some instances [19] though not in others [20,21], Our assessment of the present case, based on the clinical picture, laboratory findings and morphometric analysis, is that this pa tient's hypereosinophilia was not leukaemic.…”

Section: Discussionmentioning

confidence: 66%

Hypereosinophilic Syndrome: Case Report and Morphometric Study

Sokol

Hudson²,

Brown³

et al. 1988

Acta Haematol

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A clinical study of a patient with hypereosinophilic syndrome is presented. The blood eosinophil count reached 273 × 10⁹ cells per litre. There were only mild symptoms but skin, lung and heart were affected. There was a good response to treatment. In a morphometric analysis, the patient’s eosinophils were compared with those of 9 healthy subjects, using appropriate multiple comparison procedures. The patient’s eosinophils showed significantly increased cell size and surface area. Mitochondrial volume and surface area were also increased and there were more mitochondrial profiles per section. The eosinophil granules showed a striking reduction in total volume, both the number of profiles and the profile area of individual granules being decreased. These findings are consistent with inappropriate activation and degranulation, a relatively orderly degranulation perhaps accounting for the mild symptoms. This investigation illustrates the value of morphometry in quantitating cellular changes in haematological disorders.

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Eosinophilic leukaemia: morphological, cytochemical, and electron microscopic studies.

Cited by 18 publications

References 32 publications

Eosinophilic differentiation of the human promyelocytic leukemia cell line, HL-60.

Eosinophilic differentiation of the human promyelocytic leukemia cell line, HL-60.

The Association of Eosinophilia with Lymphoblastic Leukaemia or Lymphoma: a Study of Seven Patients

Hypereosinophilic Syndrome: Case Report and Morphometric Study

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