Eosinophil peroxidase-dependent hydroxyl radical generation by human eosinophils

McCormick, Michael L.; Roeder, T.L.; Railsback, Michelle A.; Britigan, B. E.

doi:10.1016/0891-5849(93)90485-d

Cited by 20 publications

(23 citation statements)

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“…It has been shown that generation of O 2 9 -and H 2 O 2 plays an important role in host defense against parasite infection [36]. Excess NO and O 2 9…”

Section: Discussionmentioning

confidence: 99%

Curcumin reduces oxidative and nitrative DNA damage through balancing of oxidant–antioxidant status in hamsters infected with Opisthorchis viverrini

Pinlaor

Yongvanit

Prakobwong

et al. 2009

Molecular Nutrition Food Res

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Opisthorchis viverrini (OV) infection is endemic in northeastern Thailand. We have previously reported that OV infection induces oxidative and nitrative DNA damage via chronic inflammation, which contributes to the disease and cholangiocarcinogenesis. Here, we examined the effect of curcumin, an antioxidant, on pathogenesis in OV-infected hamsters. DNA lesions were detected by double immunofluorescence and the hepatic expression of oxidant-generating and antioxidant genes was assessed by quantitative RT-PCR analysis. Dietary 1.0% curcumin significantly decreased OV-induced accumulation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), an oxidative DNA lesion, and 8-nitroguanine, a nitrative DNA lesion, in the nucleus of bile duct epithelial and inflammatory cells. Expression of oxidant-generating genes (inducible nitric oxide synthase; iNOS, its nuclear transcriptional factor, NF-kappaB, and cyclooxygenase-2), and plasma levels of nitrate, malondialdehyde, and alanine aminotransferase, were also decreased in curcumin-treated group. In contrast, curcumin increased the mRNA expression of antioxidant enzymes (Mn-superoxide dismutase and catalase), and ferric-reducing anti-oxidant power in the plasma. In conclusion, curcumin reduced oxidative and nitrative DNA damage by suppression of oxidant-generating genes and enhancement of antioxidant genes, leading to inhibition of oxidative and nitrative stress. Therefore, curcumin may be used as a chemopreventive agent to reduce the severity of OV-associated diseases and the risk of cholangiocarcinoma (CCA).

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“…It has been shown that generation of O 2 9 -and H 2 O 2 plays an important role in host defense against parasite infection [36]. Excess NO and O 2 9…”

Section: Discussionmentioning

confidence: 99%

Curcumin reduces oxidative and nitrative DNA damage through balancing of oxidant–antioxidant status in hamsters infected with Opisthorchis viverrini

Pinlaor

Yongvanit

Prakobwong

et al. 2009

Molecular Nutrition Food Res

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“…The accumulation of oxidative DNA damage may be associated with the production of reactive oxygen and nitrogen species in O. viverrini infection. Superoxide anion radical (O 2 Á À ) is derived from eosinophils (27) and macrophages (28) and dismutated to H 2 O 2 , which induces metaldependent 8-oxodG formation (29). Our previous results showed that O. viverrini -infected hamsters had higher inducible NO synthase levels and increased 8-oxodG formation in the epithelial bile duct and in inflammatory cells at the site of infection (17,30).…”

Section: Discussionmentioning

confidence: 99%

Urinary 8-Oxo-7,8-Dihydro-2′-Deoxyguanosine in Patients with Parasite Infection and Effect of Antiparasitic Drug in Relation to Cholangiocarcinogenesis

Thanan

Murata

Pinlaor

et al. 2008

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Parasite infection of Opisthorchis viverrini is a major risk factor for cholangiocarcinoma. Our previous immunohistochemical studies showed that O. viverrini infection induced oxidative DNA lesions in the bile duct epithelium during cholangiocarcinoma development. The current study assessed the levels of 8-oxo-7,8-dihydro-2 ¶-deoxyguanosine (8-oxodG), an oxidative DNA lesion, in the urine and leukocytes of O. viverrini -infected subjects and cholangiocarcinoma patients. Forty-nine O. viverrini -infected patients, 55 cholangiocarcinoma patients, and 17 healthy controls were enrolled in the study. We measured 8-oxodG levels in the urine and leukocytes of these subjects using an electrochemical detector coupled to high-performance liquid chromatography. O. viverrini -infected patients were assessed before treatment and 2 months and 1 year after praziquantel treatment. Urinary 8-oxodG levels were significantly higher in cholangiocarcinoma patients (6.83 F 1.00 Mg/g creatinine) than in O. viverrini -infected patients (4.45 F 0.25 Mg/g creatinine; P < 0.05) and healthy subjects (3.03 F 0.24 Mg/g creatinine; P < 0.01) and higher in O. viverrini -infected subjects than in healthy subjects (P < 0.01). The urinary 8-oxodG levels in O. viverrini -infected patients significantly decreased 2 months after praziquantel treatment and were comparable with levels in healthy subjects 1 year after treatment. Urinary 8-oxodG levels were significantly correlated with leukocyte 8-oxodG levels, plasma nitrate/nitrite levels, and aspartate aminotransferase activity. In conclusion, this study, in addition to our previous studies, indicates that 8-oxodG formation by parasite infection may play an important role in cholangiocarcinoma development. Urinary 8-oxodG may be a useful biomarker to monitor not only infection but also carcinogenesis. (Cancer Epidemiol Biomarkers Prev 2008;17(3):518 -24)

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“…Eosinophils are exceptionally rich in peroxidase, e.g., EPO, which is present in the matrix of eosinophil granules at 15 g/10 6 eosinophils (9, 35). In systems using purified EPO, superoxide, and hydrogen peroxide, the generation of potent cytotoxic species such as the hydroxyl radical in eosinophils has been observed (31). When combined with H 2 O 2 and halides (for example, chloride, iodide, or bromide), EPO catalyzes the generation of highly cytotoxic hypochalous acids (22).…”

Section: Discussionmentioning

confidence: 99%

Bactericidal Activity of Human Eosinophilic Granulocytes againstEscherichia coli

Persson¹,

Andersson²,

Bodelsson

et al. 2001

Infect Immun

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Eosinophils participate in allergic inflammation and may have roles in the body's defense against helminthic infestation. Even under noninflammatory conditions, eosinophils are present in the mucosa of the large intestine, where large numbers of gram-negative bacteria reside. Therefore, roles for eosinophils in host defenses against bacterial invasion are possible. In a system for bacterial viable counts, the bactericidal activity of eosinophils and the contribution of different cellular antibacterial systems against Escherichia coli were investigated. Eosinophils showed a rapid and efficient killing of E. coli under aerobic conditions, whereas under anaerobic conditions bacterial killing decreased dramatically. In addition, diphenylene iodonium chloride (DPI), an inhibitor of the NADPH oxidase and thereby of superoxide production, also significantly inhibited bacterial killing. The inhibitor of nitric oxide (NO) production L-N 5 -(1-iminoethyl)-ornithine dihydrochloride did not affect the killing efficiency, suggesting that NO or derivatives thereof are of minor importance under the experimental conditions used. To investigate the involvement of superoxide and eosinophil peroxidase (EPO) in bacterial killing, EPO was blocked by azide. The rate of E. coli killing decreased significantly in the presence of azide, whereas addition of DPI did not further decrease the killing, suggesting that superoxide acts in conjunction with EPO. Bactericidal activity was seen in eosinophil extracts containing granule proteins, indicating that oxygen-independent killing may be of importance as well. The findings suggest that eosinophils can participate in host defense against gram-negative bacterial invasion and that oxygen-dependent killing, i.e., superoxide acting in conjunction with EPO, may be the most important bactericidal effector function of these cells.

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Eosinophil peroxidase-dependent hydroxyl radical generation by human eosinophils

Cited by 20 publications

References 0 publications

Curcumin reduces oxidative and nitrative DNA damage through balancing of oxidant–antioxidant status in hamsters infected with Opisthorchis viverrini

Curcumin reduces oxidative and nitrative DNA damage through balancing of oxidant–antioxidant status in hamsters infected with Opisthorchis viverrini

Urinary 8-Oxo-7,8-Dihydro-2′-Deoxyguanosine in Patients with Parasite Infection and Effect of Antiparasitic Drug in Relation to Cholangiocarcinogenesis

Bactericidal Activity of Human Eosinophilic Granulocytes againstEscherichia coli

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