Eosinophil cationic protein and interleukin‐8 levels in bronchial lavage fluid from children with asthma and infantile wheeze

Marguet, Christophe; Dean, Tara; Basuyau, Jean-Pierre; Warner, John O.

doi:10.1034/j.1399-3038.2001.012001027.x

Cited by 50 publications

(62 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our data show that the action of 15-d-PGJ 2 on recruited, newly activated T cells (which are quite prevalent during an asthmatic response) serves to induce the production of IL-8. This agrees with published findings that IL-8 is, in fact, found in much higher concentrations in the epidermis of psoriasis patients as well as in the bronchoalveolar lavage fluid of asthmatics (44,45). This increased IL-8 then induces the chemotaxis and subsequent activation of cells such as neutrophils.…”

Section: Discussionsupporting

confidence: 82%

See 1 more Smart Citation

15-Deoxy-Δ12,1412,14-PGJ2 Induces IL-8 Production in Human T Cells by a Mitogen-Activated Protein Kinase Pathway

Harris

Smith

Phipps

2002

The Journal of Immunology

View full text Add to dashboard Cite

Mast cells, platelets, and some macrophages are abundant sources of PGD2 and its active metabolite 15-deoxy-Δ12,14-PGJ2 (15-d-PGJ2). The lipid mediator 15-d-PGJ2 regulates numerous processes, including adipogenesis, apoptosis, and inflammation. The 15-d-PGJ2 has been shown to both inhibit as well as induce the production of inflammatory mediators such as TNF-α, IL-1β, and cyclooxygenase, mostly occurring via a nuclear receptor called peroxisome proliferator-activated receptor-γ (PPAR-γ). Data concerning the effects of 15-d-PGJ2 on human T cells and immune regulation are sparse. IL-8, a cytokine with both chemotactic and angiogenic effects, is produced by T lymphocytes following activation. Whether 15-d-PGJ2 can regulate the production of IL-8 in T cells in unknown. Interestingly, 15-d-PGJ2 treatment of unstimulated T cells induces cell death. In contrast, in activated human T lymphocytes, 15-d-PGJ2 does not kill them, but induces the synthesis of IL-8. In this study, we report that 15-d-PGJ2 induced a significant increase in both IL-8 mRNA and protein from activated human T lymphocytes. The induction of IL-8 by 15-d-PGJ2 did not occur through the nuclear receptor PPAR-γ, as synthetic PPAR-γ agonists did not mimic the IL-8-inducing effects of 15-d-PGJ2. The mechanism of IL-8 induction was through a mitogen-activated protein kinase and NF-κB pathway, as inhibitors of both systems abrogated IL-8 protein induction. Therefore, 15-d-PGJ2 can act as a potent proinflammatory mediator in activated T cells by inducing the production of IL-8. These findings show the complexity with which 15-d-PGJ2 regulates T cells by possessing both pro- and anti-inflammatory properties depending on the activation state of the cell. The implications of this research also include that caution is warranted in assigning a solely anti-inflammatory role for 15-d-PGJ2.

show abstract

Section: Discussionsupporting

confidence: 82%

“…Neutrophils play a major role in allergic inflammation (46). In fact, the level of neutrophil-mediated inflammation in bronchoalveolar lavage fluid often reflects the severity of asthma in the patient (44). Therefore, 15-d-PGJ 2 could be playing an integral role in the development of inflammation associated with allergies and asthma.…”

Section: Discussionmentioning

confidence: 99%

15-Deoxy-Δ12,1412,14-PGJ2 Induces IL-8 Production in Human T Cells by a Mitogen-Activated Protein Kinase Pathway

Harris

Smith

Phipps

2002

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…These changes assist in the recruitment of leukocytes, including eosinophils, and neutrophils [7][8][9][10][11][12]. Neutrophil recruitment into the airways of asthmatic children has been associated with the release of the potent neutrophil attractant interleukin (IL)-8 [13,14]. However, the chemoattractants involved in eosinophil trafficking remain to be shown.…”

mentioning

confidence: 99%

Role of the chemokines RANTES, monocyte chemotactic proteins-3 and -4, and eotaxins-1 and -2 in childhood asthma

Rojas‐Ramos¹,

Avalos²,

Pérez-Fernandez³

et al. 2003

European Respiratory Journal

View full text Add to dashboard Cite

Eosinophil recruitment into the airways is a feature of asthma in children. However, the mechanisms by which these cells migrate into the airways are not fully understood. The present study investigated the presence of the eosinophilactivating chemokines regulated on activation, normal T-cell expressed and secreted (RANTES), monocyte chemotactic proteins (MCP)-3 and -4, and eotaxins-1 and -2 in the bronchoalveolar lavage (BAL) fluid obtained from both asthmatic (n=10, age 6-10 yrs) and normal children (n=10, age 5-10 yrs).Measurements of chemokines in BAL fluid showed that levels of RANTES, MCPs-3 and -4, and eotaxins-1 and -2 were significantly increased in fluid obtained from asthmatic children when compared with normal children. Among the different chemokines, RANTES was the cytokine released in greatest quantities in BAL fluid from asthmatic children. There was a significant correlation between the concentrations of MCP-4 and eosinophil numbers in BAL fluid and a trend between both chemokines MCP-3 and eotaxin-2 and eosinophils.Interestingly, the levels of most chemokines correlated with one another. These findings suggest that RANTES monocyte chemotactic proteins-3 and -4, and eotaxins-1 and -2 may regulate eosinophil trafficking into the airways of asthmatic children in a coordinated manner. Eur Respir J 2003; 22: 310-316.

show abstract

“…Astımlı hastalardaki kullanımı, ECP'nin hışıltılı infantlarda gösterge olarak kullanılabilirliği ile ilgili çalışmaları beraberinde getirmiştir. Serum ECP düzeyinin hışıltılı alt solunum yolu enfeksiyonlarında yükseldiği ve bunlardaki düzeyin sağlıklı çocuklara göre anlamlı olarak daha yüksek olduğu, ECP düzeyi yüksek olan infantlarda hışıltılı alt solunum yolu enfeksiyonlarının gelişme riskinin daha yüksek olduğu ve bundan dolayı ECP düzeylerinin, astım gelişimi için risk taşıyan bireylerin izlenmesinde yararlı bir araç olarak kullanılabileceği ileri sürülmektedir (9,29). Eozinofilik aktivasyonun serumdaki göstergelerinin çocuklardaki havayolu inflamasyonunun derecesi ile korele olduğunun gösterilmesi, ECP'nin havayollarındaki inflamasyonun indirekt ve invaziv olmayan bir göstergesi olarak kullanı-labileceği fikrini doğurmuştur (30).…”

Section: Discussionunclassified

İnfantlarda Sık Hışıltı Gelişimini Öngörmede Erken Yaşam Belirteçleri; Gizemli Bir Yolda Eski Dost, ‘’Eozinofil’’ ile Devam Etmek

Turhan¹,

Arikoğlu²,

Tezol³

et al. 2017

Asthma Allergy Immunol

View full text Add to dashboard Cite

ÖZGiriş: Astımlı olguların birçoğunda hışıltı semptomu yaşamın ilk yıllarında başlar. Çocukluk çağında astım gelişiminde rol oynayan faktörleri değerlendiren çalışmalar olmasına rağmen, erken çocukluk döneminde sık hışıltı gelişimini öngören değerler hâlâ net değildir. Çalışmamızın amacı, hışıltı atakları ile ailesel, prenatal ve postnatal risk faktörleri arasındaki ilişkiyi ve serum eozinofilik katyonik protein (ECP) ve eozinofil düzeylerinin sık hışıltı ataklarını öngörmedeki duyarlılığını araştırmaktır. Gereç ve Yöntem:Çalışmaya, yaşları 1-36 ay arasında değişen ve akut hışıltı atağı ile başvuran 57 çocuk ve 50 sağlam çocuk alındı. Başvurudan sonraki bir yıllık sürede ≥3 akut hışıltı atağı geçiren çocuklar sık hışıltı grubu, daha az sayıda atak geçirenler seyrek hışıltı grubu olarak tanımlandı. Hışıltı risk faktörlerini ayrıntılı sorgulayan anket formu dolduruldu. Tüm hastalardan serum total IgE, ECP, eozinofil sayısı, besin ve inhalan spesifik IgE düzeyleri ve RSV/ adenovirus serolojisi için kan örnekleri alındı, deri prik testi yapıldı. Öyküde besin allerjisi şüphesi olan olgularda besin provokasyon testleri uygulandı. Bulgular: Sık hışıltı grubunda erkek cinsiyet (p=0.024), düşük sosyoekonomik düzey (p=0.046), 4. aydan önce formula mama ABSTRACT Objective: In most cases of asthma, wheezing symptoms start in early childhood. Although there are studies evaluating the factors that play a role in the development of childhood asthma, the predictive markers for frequent wheezing in early childhood are still unclear. The aim of our study was to investigate the relation between the wheezing episodes and the familial, prenatal, and postnatal risk factors, and the sensitivity of serum eosinophilic cationic protein (ECP) and eosinophil levels for predicting frequent wheezing. Materials and Methods:Fifty-seven children with acute wheezing and fifty healthy children aged between 1-36 months were included in the study. The children who had ≥ 3 episodes of wheezing in the next year after presentation were classified as the frequent wheezing group and those with fewer episodes were classified as the infrequent wheezing group. Administration of a comprehensive questionnaire about risk factors for wheezing; blood sampling for serum total IgE, ECP, eosinophil count, food and inhaled specific IgE levels and RSV/adenovirus serology; and skin prick tests were performed. Oral provocation tests were applied in cases of suspected food allergy.Results: Male gender (p=0.024), low socioeconomic level (p=0.046), initiation of milk formula (p=0.009) and eggs (p=0.018) before the fourth month, the presence of mold / humidity at home (p=0.023),

show abstract

Eosinophil cationic protein and interleukin‐8 levels in bronchial lavage fluid from children with asthma and infantile wheeze

Cited by 50 publications

References 29 publications

15-Deoxy-Δ12,1412,14-PGJ2 Induces IL-8 Production in Human T Cells by a Mitogen-Activated Protein Kinase Pathway

15-Deoxy-Δ12,1412,14-PGJ2 Induces IL-8 Production in Human T Cells by a Mitogen-Activated Protein Kinase Pathway

Role of the chemokines RANTES, monocyte chemotactic proteins-3 and -4, and eotaxins-1 and -2 in childhood asthma

İnfantlarda Sık Hışıltı Gelişimini Öngörmede Erken Yaşam Belirteçleri; Gizemli Bir Yolda Eski Dost, ‘’Eozinofil’’ ile Devam Etmek

Contact Info

Product

Resources

About