Eosinophil activation by lymphokines and T cell clone products in the rat

Veith, Monica C.; Pestel, Joël; Loiseau, Sylvie; Capron, Moníque; Càpron, André

doi:10.1002/eji.1830151218

Cited by 10 publications

(3 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Veith et al [26] have shown an enhancement of antibody-mediated killing using T cell derived factors, and Auriault et al [6] and Capron et al [27] have described similar ef fects with products derived from mast cells and para sites. Furthermore, our own results with FMLP indi cated that the ADCC response of eosinophils from M.corti infected rats was enhanced, albeit to levels comparable with those of unstimulated eosinophils from animals treated with Sephadex-saline.…”

Section: Discussionmentioning

confidence: 92%

Effect of Chemotactic Agents on Rat and Guinea Pig Eosinophil Cytotoxicity in vitro

Musgrove¹,

Cook²

1988

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Purified guinea pig and rat peritoneal eosinophils were examined for their ability to mediate an antibody-dependent cell cytotoxic reaction against ⁵¹Cr-labelled chick red blood cells in the presence of N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP), leukotriene B₄, eosinophil chemotactic factor of anaphylaxis, or histamine. Guinea pig eosinophils were induced either by repeated intraperitoneal injections of polymyxin B sulphate or by a single intraperitoneal injection of saline. Using subagglutinating concentrations of antibody, the antibody-dependent cell cytotoxic response was significantly enhanced with FMLP, leukotriene B4, and eosinophil chemotactic factor of anaphylaxis. Histamine was without effect. Rat eosinophils were induced either by three intravenous injections of Sephadex G-200 followed by saline intraperitoneally, by a single intraperitoneal injection of saline, or by infection with the parasite Mesocestoides corti. None of the materials examined was able to enhance the cytotoxic activity of the rat eosinophil preparations over the range tested, in the presence of subagglutinating concentrations of antibody. The antibody-dependent cell cytotoxic response of eosinophils from infected animals was significantly reduced compared with those from Sephadex/saline-treated rats. Incubation of eosinophils obtained from infected animals with FMLP in the presence of agglutinating concentrations of antibody restored eosinophil cytotoxicity. None of the other materials was active. It is concluded that the ability of rat and guinea pig eosinophils to be activated in vitro is partly species related and partly due to the method of cell induction. Differences between the two species may be important when establishing animal models of eosinophil function.

show abstract

Section: Discussionmentioning

confidence: 92%

Effect of Chemotactic Agents on Rat and Guinea Pig Eosinophil Cytotoxicity in vitro

Musgrove¹,

Cook²

1988

Int Arch Allergy Immunol

View full text Add to dashboard Cite

show abstract

“…T cell clones (G5 or E23) (1 x 106-4 x lo6 cells/ml) were stimulated for 24 h in the presence of irradiated (2000 rads = 20 Gy) syngeneic APC with or without IPSm (50 pglml; [29]). After washings, T cell clones were maintained for a further 24 h in RPMI containing 5% FCS or in serum-free RPMI.…”

Section: Preparation Of T Cell Clones Supernatants (Sn)mentioning

confidence: 99%

Schistosoma mansoni‐specific rat T cell clones. II. Different effects of adult worm‐specific T cell clones in immunocompetent and nude infected rats

Pestel

Dissous

Louis³

et al. 1989

Eur J Immunol

Self Cite

View full text Add to dashboard Cite

The in vivo functional activities of two highly proliferating helper rat T cell clones (E23 and G5) specific for the excretory-secretory antigens of Schistosoma mansoni adult worms were investigated. When injected into infected immunocompetent rats, both clones increased the antibody response against the 30-40-kDa schistosomulum surface antigens, but failed to induce an immune protection. In contrast, when the same clones were injected into infected nude rats, a high degree of protection was obtained. In this latter case the absence of detectable specific antibody response, whether of IgE or IgG isotype, suggested that parasites were destroyed by an antibody-independent mechanism, i.e. macrophages activation by lymphokines. Indeed supernatants obtained from T cell clones specifically restimulated with schistosome antigens expressed a macrophage activated activity similar to interferon-gamma. Following incubation with these supernatants or with the active fractions, macrophages exhibited a significant schistosomulicidal activity and both clones were shown to transfer an antigen-specific delayed-type hypersensitivity reaction to normal rats. Taken together these results demonstrate that, depending on the immune status of the host, antigen-specific T cell clones can function differently and consequently that one function associated with one type of lymphokine could be favored.

show abstract

“…The analysis of the various components involved in immunity directed towards Schistosoma mansoni has revealed that its essential features are the participation of antibodies, in cooperation with phagocytic cells or platelets, and thymodependency (6). The analysis of the target antigens present on the schistosomulutn surface, recognized by cytotoxic monoclonal or polyclonal antibodies, has emphasized the preponderant role played by four antigens of 38 (10), 28 (2), 22, and 26 kilodaltons (kDa) (1). Among these molecules, that of 28 kDa is of particular interest since it is present in the in vitro translation products of adult worm RNA (2).…”

mentioning

confidence: 99%

Helper T cells induced by a purified 28-kilodalton antigen of Schistosoma mansoni protect rats against infection

Auriault¹,

Balloul²,

Pierce³

et al. 1987

Infect Immun

View full text Add to dashboard Cite

Schistosoma mansoni adult worm 28-kilodalton (kDa) proteins were separated on polyacrylamide slab gels, recovered by electrophoretic elution, and used to immunize Fischer rats. After the second or third inijection, inguinal lymph node T lymphocytes were propagated in vitro for 4 weeks in the presence of syngeneic antigen-presenting cells and adult worm antigens in medium containing interleukin-2. After this period of culture, 99% of the cells expressed the W3/13+ surface phenotype and 93% of the cells expressed the W3/25+ surface phenotype. These cells were then tested for their in vivo functional activity after transfer to Fischer rats that had been either infected with S. mansoni cercariae or immunized with the 28-kDa purified protein. In each case, an increase of S. mansoni-specific antibodies was observed. Whereas anti-28-kDa antibodies were only detectable at day 40 postinfection in controls iu'ected with normal T lymphocytes, they appeared as early as day 13 postinfection when the animals received 28-kDa protein-specific T lymphocytes. This led to an effective protection of infected rats (45 to 85%) which correlated with the increase in S. mansoni-specific antibodies. These results therefore demonstrate that the 28-kDa protein possesses epitopes capable of activating helper T cells, which confer a strong protective immunity by enhancing the production of cytotoxic antibodies. The stimulation of the 28-kDa-specific T cells with recombinant proteins suggests that the major epitopes are located toward the carboxylic end of the molecule.

show abstract

Eosinophil activation by lymphokines and T cell clone products in the rat

Cited by 10 publications

References 43 publications

Effect of Chemotactic Agents on Rat and Guinea Pig Eosinophil Cytotoxicity in vitro

Effect of Chemotactic Agents on Rat and Guinea Pig Eosinophil Cytotoxicity in vitro

Schistosoma mansoni‐specific rat T cell clones. II. Different effects of adult worm‐specific T cell clones in immunocompetent and nude infected rats

Helper T cells induced by a purified 28-kilodalton antigen of Schistosoma mansoni protect rats against infection

Contact Info

Product

Resources

About