Eobania vermiculata as a potential indicator of nitrate contamination in soil

Cofone, Rita; Carraturo, Federica; Capriello, Teresa; Libralato, Giovanni; Siciliano, Antonietta; Giudice, Carmela Del; Maio, Nicola; Guida, Marco; Ferrandino, Ida

doi:10.1016/j.ecoenv.2020.111082

Cited by 9 publications

(8 citation statements)

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“…In E1d cells, only fucosylated residuals were present, as detected by AAA, whereas in E2d cells galactosaminylated (detected by SBA) and glycosaminylated (detected by WGA) residuals were also present. In the The decrease of mucus in cells in snails treated with pollutants has been observed previously (Cofone et al, 2020). This can be due to both reduction in the synthesis of mucus or increase of secretion rates that do not allow mucus to accumulate in the cytoplasm.…”

Section: Discussionmentioning

confidence: 78%

“…The main site for the accumulation of pollutants is the gastric gland and several biomarkers have been proposed from this organ (e.g., Itziou & Dimitriadis, 2011Regoli et al, 2006). Effects have been observed also in the foot (Ali & Said, 2019;Cofone et al, 2020;El-Khayat et al, 2015;Peña et al, 2017), being the main organ to encounter environmental pollutants during locomotion, and alterations such as increase of the cells in the lining outer epithelium or their disintegration, increased gaps and loss of cellular integrity, formation of deeper folds, increased gaps, loss of cellular integrity, and laxity of muscle fibers. Subepidermal mucous glands are also affected, since their number decreases and appear narrower and empty because of increased secretion.…”

Section: Introductionmentioning

confidence: 99%

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Aluminum exposure alters the pedal mucous secretions of the chocolate‐band snail, Eobania vermiculata (Gastropoda: Helicidae)

Guglielmi,

Mastrodonato,

Semeraro

et al. 2024

Microscopy Res & Technique

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Aluminum (Al) is used in everyday life and present in food drugs, packaging, industry, and agriculture. Although it is the most common metal in the Earth crust, a correlation has been demonstrated between its presence and various pathologies, even serious ones, especially of a neurological type. However, there is a histological gap regarding the role Al can have in contact with the covering and secreting epithelia. The alterations of the ventral and dorsal foot mucocytes and their secretions of the snail Eobania vermiculata caused by Al were investigated in situ by histochemical and lectin‐histochemical techniques. Administration to different experimental groups took place for 3 and 9 days with 50 and 200 μM of AlCl3. Several types of mucocytes were detected with a prevalent secretion of acid glycans in the foot of E. vermiculata. Sulfated glycans prevail in the dorsal region, with one type showing only fucosylated residues and another also having galactosaminylated and glycosaminylated residues. Carboxylated glycans prevail in the ventral region, with presence of galactosaminylated, glycosaminylated, and fucosylated residuals in both cells. Snails treated presented a general decrease of mucin amount in the secreting cells and affected the mucus composition. These changes could alter the rheological and functional properties of the mucus with possible implications for the health of the treated animals.Research Highlights Snails were fed with Al‐contaminated lettuce at different concentrations. In the foot mucocytes produced mucus with prevailing acidic glycans. In the treated resulted a reduction in the amount of mucus and an alteration of glycan composition.

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Section: Discussionmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Aluminum exposure alters the pedal mucous secretions of the chocolate‐band snail, Eobania vermiculata (Gastropoda: Helicidae)

Guglielmi,

Mastrodonato,

Semeraro

et al. 2024

Microscopy Res & Technique

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“…However, they are present in very large quantities after sudden discharges, promoting the proliferation of algae and photosynthetic microorganisms that reduce light penetration into deep water layers. The decomposition of dead algae also leads to oxygen consumption, resulting in the oxygen concentration in the water sufficiently low as to lead to anaerobic conditions that can result in accumulation of ammonia and nitrite compounds that might intoxicate aquatic fauna in Oued Guigou (Bjerregaard et al, 2018; Cofone et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

“…The decomposition of dead algae also leads to oxygen consumption, resulting in the oxygen concentration in the water sufficiently low as to lead to anaerobic conditions that can result in accumulation of ammonia and nitrite compounds that might intoxicate aquatic fauna in Oued Guigou (Bjerregaard et al, 2018;Cofone et al, 2020).…”

Section: Re Sults and Discussionmentioning

confidence: 99%

Assessment of heavy metal and organic pollution of a salmonid ecosystem: Case of the Oued Guigou River, Middle Atlas (Morocco)

et al. 2023

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The present study focused on evaluating the quality of the Oued Guigou River located in Middle Atlas (Morocco) by studying relevant physicochemical and metallic parameters. Samples for determining the organic matter and trace metal elements trapped in water samples were collected at upstream of Oued Guigou between December 2020 and April 2021. The samples were analysed for physicochemical quality, while the trace metal elements (lead, zinc, copper, chromium, iron, nickel, calcium and magnesium) were determined using ICP‐AES analysis. The results indicated significant organic pollution, as well as standard deviation and average changes in the values of the studied metals, most likely attributable to domestic effluents. The metal content in particular exhibited high values exceeding the recommended Moroccan standard limits, suggesting domestic and agricultural pollution is a potential threat to both the receiving rivers and their associated lakes and reservoirs.

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“…Total DNA was extracted through denaturation for 10 min at 98 °C: samples were centrifuged samples at 8,000 rpm for 5 min at 4 °C, and supernatant was recovered. PCR reactions were carried out in a TECHNE Prime Thermal Cycler employing universal primers, with a 700 bp amplicon size, complementary to the 16S rRNA gene’s conserved V3–V6 regions [ 32 , 33 ]. The selected oligos were synthetized by Biofab Research srl, and PCR reactions were carried out according to Carraturo et al (2021) [ 32 ].…”

Section: Methodsmentioning

confidence: 99%

Exposure of Buffalo Milkers to Pathogenic Bacteria and Characterization of Isolated Methicillin-Resistant Staphylococcus spp.

Carraturo

Alterisio

Guccione

et al. 2022

IJERPH

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The research was focused on the surveillance of the exposure of buffalo milkers in contact with both animals and potentially contaminated equipment, pointing attention on the diffusion of antibiotic-resistant Staphylococcus spp. The monitoring was performed for 12 months, allowing the collection of 600 raw milk and buffalo udder surface samples, 192 milking lanes, 400 milking clusters, 160 personal protective equipment (PPEs) and electronic devices surface samples in contact with the workers of four milking parlors located in Southern Italy. The analysis of the milk samples evidenced the highest exposure to the bacteria considered (and mainly to S. aureus) from late winter–spring seasons onward. The possible risk arising from buffalo udder, milking clusters, and lines were instead considered rather stable along the entire period of sampling. The PPEs turned out to be a source of contamination for milkers mainly during the spring and summer periods. The analysis for oxacillin/methicillin resistance revealed in all the farms enrolled an overall amount of 37.5% of Staphylococci strains (belonging to S. aureus, S. haemolyticus, S. pseudintermedius, S. chromogenes species) resistant both to methicillin and oxacillin. The investigation demonstrated that the potential transfer of pathogenic bacteria to humans would have a better chance to occur at milk resumption time (since late winter–spring onward) when the number of animals to be milked is greater and the activity in the milking parlor is more challenging. At the same time, the findings seem to point out that the potential risk may be worsened by a significant presence of oxacillin/methicillin-resistant Staphylococci, potentially resulting from irrational use of antibiotics.

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Eobania vermiculata as a potential indicator of nitrate contamination in soil

Cited by 9 publications

References 50 publications

Aluminum exposure alters the pedal mucous secretions of the chocolate‐band snail, Eobania vermiculata (Gastropoda: Helicidae)

Aluminum exposure alters the pedal mucous secretions of the chocolate‐band snail, Eobania vermiculata (Gastropoda: Helicidae)

Assessment of heavy metal and organic pollution of a salmonid ecosystem: Case of the Oued Guigou River, Middle Atlas (Morocco)

Exposure of Buffalo Milkers to Pathogenic Bacteria and Characterization of Isolated Methicillin-Resistant Staphylococcus spp.

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