Enzymverteilungsmuster in normalen und pathologischen weißen Blutzellen des Menschen

Löhr, G. W.; Waller, H. D.; Bock, H. E.

doi:10.1007/978-3-642-96027-7_164

Cited by 6 publications

(4 citation statements)

References 11 publications

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“…In complete homogenates of human granulocytes, Löhr and Waller [42] have found an ATP'ase activity similar to that found here.…”

Section: Atp'ase (Mg Activated)supporting

confidence: 64%

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Leukocyte Energy Metabolism III. Anaerobic and Aerobic ATP Production and Related Enzymes

Jemelin¹,

Frei²

1970

Enzymol Biol Clin

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We have measured anaerobic and aerobic energy metabolism in leukocytes from human and animal blood, using several methods. We have taken special care to correct our results for an unavoidable contamination with thrombocytes, which has been neglected in all previous work. White cells were isolated by a method combining sedimentation through Dextran with low speed centrifugations. A pellet of thrombocytes was prepared in parallel from the same blood. Mitochondria were isolated by differential centrifugation in 0.25 M sucrose. Firstly, we have specially studied two cytosolic glycolytic enzymes, phosphoglycerate kinase and pyruvate kinase, both of which catalyze a reaction producing ATP. These two enzymes are very active in leukocytes. Other nucleotides, such as CDP, GDP and UDP can also be phosphorylated. Secondly, we have measured the activity of several mitochondrial enzymes, the most active being α-glycerophosphate oxidase. Using an enzymatic test, we have measured the amount of ATP produced in oxidative phosphorylation; in these experiments it was necessary to consider the activity of adenylate kinase as well as correcting for thrombocyte contamination. Several metabolites in direct or indirect relation with the Krebs cycle were used as oxidizable substrates: glutamate, succinate and α-glycerophosphate gave the best results. Except for amytal, inhibitors of oxidative phosphorylation also inhibit ATP production in leukocyte mitochondria. In conclusion, it can be said that oxidative phosphorylation exists in leukocytes, but that the phosphorylation steps in glycolysis produce 1,000 to 10,000 times more ATP than aerobic energy metabolism.

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“…In complete homogenates of human granulocytes, Löhr and Waller [42] have found an ATP'ase activity similar to that found here.…”

Section: Atp'ase (Mg Activated)supporting

confidence: 64%

“…In complete homogenates of human leukocytes, Löhr and Waller [42] have measured an activity for GDH at 25°C, which is 5 times lower than our value,…”

Section: Measurements Of This Intramitochondrial Enzyme Have Also Percontrasting

confidence: 41%

Leukocyte Energy Metabolism III. Anaerobic and Aerobic ATP Production and Related Enzymes

Jemelin¹,

Frei²

1970

Enzymol Biol Clin

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“…The increased DNA synthesis and increased mitotic frequency in lymphocytes after addition of L-alanine to the culture medium indicate that the endo genous production of L-alanine is too low' and that an exogenous supply is necessary for optimal growth. The requirement for L-alanine may be related to the very low level of alanine amino transferase in lym phocytes [Schmidt and Schmidt, 1960;Löhr, 1961]. In contrast to lymphocytes from different species, rat liver cells were not dependent on L-alanine for optimal DNA synthesis in our in vitro system [Söder, personal commun.…”

Section: Discussionmentioning

confidence: 99%

Mitotic Activity of Thymocytes in a Synthetic Tissue Culture Medium. Effect of <i>L</i>-AIanine

Sandberg¹,

Ernström²

1979

Int Arch Allergy Immunol

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DNA synthesis in guinea pig thymocytes suspended in RPMI 1640 medium increased to a peak after 4–5 h in culture and was followed by increased mitotic activity, indicating that many thymocytes in S phase proceeded through G2 into mitosis. Addition of L-alanine to the medium markedly increased the DNA synthesis within 1 h and the mitotic frequency from 6 h. The increase in DNA synthesis when L-alanine was present in the medium was thus caused by an increased number of cells in S phase. Human thymocytes cultured in RPMI 1640 for 18 h had a low mitotic frequency. Addition of L-alanine immediately started DNA synthesis in the arrested thymocytes resulting in increased mitotic activity from 6 h later. The results show that L-alanine is a growth factor for guinea pig and human thymocytes and should be included in tissue culture media used for such cells. Growth of thymocytes in vitro was partly synchronized, and the mitotic studies indicated that many cells had entered S phase near the start of incubation.

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“…Wei tere Einblicke in den Metabolismus der Leukozyten konnten im letzten Jahrzehnt durch Bestimmungen von Enzymaktivitäten der Glykolyse gewonnen werden [3,4,7,12,19].…”

Section: Des Enzymes De La Glycolyse Dans Les Leucocytes D'enfantsunclassified

Über Enzyme der Glykolyse in Leukozyten von Kindern

Stave¹

1961

Enzymol Biol Clin

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In isolierten Leukozyten aus Vollblut und Liquor wurden die Aktivitäten folgender Enzyme bestimmt: Hexokinase (HK), Glukose-6-Phos phatdehydrogenase (G-6-PDH), Aldolase, Glyceraldehydphosphatdehydrogenase (GAPDH), Lactatdehydrogenase (LDH), Malatdehydrogenase und Glutamat- Oxalacetat-Transaminase. Unter Berücksichtigung des prozentualen Anteils der Lymphozyten zeigen Vergleiche der Blut- und Liquorleukozyten, daß die zur Isolierung der Blutleukozyten verwendete Methode die untersuchten Enzymaktivitäten nicht verändert. Es bestehen keine Differenzen der Enzymaktivitäten bei Kindern verschiedenen Alters. Die Aktivität der G-6-PDH in Lymphozyten beträgt nur 11% derjenigen in Granulozyten; hierin sind auch die Aktivitäten der GAPDH und HK stärker vermindert, die der LDH dagegen nicht. Die G-6-PDH-Aktivität der Leukozyten bei Virusinfektionen ist nicht nur infolge des höheren Lymphozytenanteils, sondern vermutlich auch infolge einer virusbedingten Einwirkung auf den Zellmetabolismus vermindert.

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Enzymverteilungsmuster in normalen und pathologischen weißen Blutzellen des Menschen

Cited by 6 publications

References 11 publications

Leukocyte Energy Metabolism III. Anaerobic and Aerobic ATP Production and Related Enzymes

Leukocyte Energy Metabolism III. Anaerobic and Aerobic ATP Production and Related Enzymes

Mitotic Activity of Thymocytes in a Synthetic Tissue Culture Medium. Effect of <i>L</i>-AIanine

Über Enzyme der Glykolyse in Leukozyten von Kindern

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