Enzymology and Molecular Biology of Aflatoxin Biosynthesis

Minto, Robert E.; Townsend, Craig A.

doi:10.1021/cr960032y

Cited by 241 publications

(162 citation statements)

References 219 publications

(558 reference statements)

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“…Aflatoxin B1 (AFB1) shows toxicity and carcinogenic effects on human and animals [1,2]. Detection of AFB1 is important in food safety, quality control, environmental analysis, and toxin sensing.…”

Section: Introductionmentioning

confidence: 99%

Aptamer based surface plasmon resonance sensor for aflatoxin B1

Sun

Zhao

2017

Microchim Acta

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The authors describe a surface plasmon resonance (SPR) based aptasensor for the carcinogenic mycotoxin aflatoxin B1 (AFB1) in a direct assay format. The aptamer is immobilized on the surface of a commercial sensor chip, and the SPR signal increases on binding of AFB1. The sensor chip can be fully regenerated by passing a flow of buffer over it upon which bound AFB1 dissociates from the aptamer. The biosensor works in the 0.4 nM to 200 nM AFB1 concentration range and has a 0.4 nM detection limit. It allows AFB1 to be determined in complex samples such as diluted red wine and beer. The assay is sensitive, and the chip is easily regenerated and stable. The method therefore overcomes certain limitations of antibody-based SPR assays and of competitive SPR assays for AFB1.

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Section: Introductionmentioning

confidence: 99%

Aptamer based surface plasmon resonance sensor for aflatoxin B1

Sun

Zhao

2017

Microchim Acta

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“…-Aspergillus versicolor, a harmful fungus, is widely found in human and animal foodstuffs. Its secondary metabolites such as aflatoxins and related sterigmatocystins are carcinogenic [1]. Therefore, a variety of secondary metabolites of A. versicolor were extensively characterized, including xanthones such as sterigmatin [2] and sterigmatocystin [3], anthraquinones such as averantin [4], averufin [5], and versicolorone [6], a terpenoid, versiol [7], lactones [8], and chromones such as aspergillitine or aspergiones A -F [9].…”

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Four New Alkaloids, Brevianamides O – R, from the Fungus Aspergillus versicolor

Yang

et al. 2010

Helvetica Chimica Acta

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Four new alkaloids, brevianamides O -R (1 -4, resp.), were isolated from the AcOEt extract of the solid-state fermented rice culture of the fungus Aspergillus versicolor. Their structures were elucidated on the basis of spectroscopic analyses.Introduction. [8], and chromones such as aspergillitine or aspergiones A -F [9]. Our previous study on the alkaloidal metabolites of the fungus A. versicolor led to the identification of five new diketopiperazine alkaloids [10]. The present study disclosed four new diketopiperazine alkaloids, brevianamides O -R (1 -4, resp.), from the AcOEt extract of the solid-statefermented rice culture of this fungus. Here, the isolation and structure elucidation of compounds 1 -4 are described.

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“…P olyketide natural products include the antibiotics erythromycin (1,2) and tetracycline (3), the immunosuppressant rapamycin (4), the cancer therapeutic daunorubicin (5), and the environmental carcinogen aflatoxin B 1 (1) (6). Polyketide synthases (PKSs) assemble these structurally diverse products from simple acyl-CoA substrates by using a catalytic cycle involving decarboxylative Claisen condensations and variable modifications such as reduction and dehydration events.…”

mentioning

confidence: 99%

Identification of a starter unit acyl-carrier protein transacylase domain in an iterative type I polyketide synthase

Crawford

Dancy

Hill

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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163

184

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Polyketides are a class of natural products that exhibit a wide range of functional and structural diversity. They include antibiotics, immunosuppressants, antifungals, antihypercholesterolemics, and cytotoxins. Polyketide synthases (PKSs) use chemistry similar to fatty acid synthases (FASs), although building block variation and differing extents of reduction of the growing polyketide chain underlie their biosynthetic versatility. In contrast to the well studied sequential modular type I PKSs, less is known about how the iterative type I PKSs carry out and control chain initiation, elongation, folding, and cyclization during polyketide processing. Domain structure analysis of a group of related fungal, nonreducing PKSs has revealed well defined N-terminal domains longer than commonly seen for FASs and modular PKSs. Predicted structure of this domain disclosed a region similar to malonyl-CoA:acyl-carrier protein (ACP) transacylases (MATs). MATs play a key role transferring precursor CoA thioesters from solution onto FASs and PKSs for chain elongation. On the basis of site-directed mutagenesis, radiolabeling, and kinetics experiments carried out with individual domains of the norsolorinic acid PKS, we propose that the Nterminal domain is a starter unit:ACP transacylase (SAT domain) that selects a C6 fatty acid from a dedicated yeast-like FAS and transfers this unit onto the PKS ACP, leading to the production of the aflatoxin precursor, norsolorinic acid. These findings could indicate a much broader role for SAT domains in starter unit selection among nonreducing iterative, fungal PKSs, and they provide a biochemical rationale for the classical acetyl ''starter unit effect.'' aflatoxin biosynthesis ͉ fatty acid synthase

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Enzymology and Molecular Biology of Aflatoxin Biosynthesis

Cited by 241 publications

References 219 publications

Aptamer based surface plasmon resonance sensor for aflatoxin B1

Aptamer based surface plasmon resonance sensor for aflatoxin B1

Four New Alkaloids, Brevianamides O – R, from the Fungus Aspergillus versicolor

Identification of a starter unit acyl-carrier protein transacylase domain in an iterative type I polyketide synthase

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