Enzymological Properties of Three Soil Hydrolases and Effects of Several Pesticides on Their Activities

Nakamura, Toshiie; Mochida, Kazuo; Ozoe, Yoshihisa; Ukawa, Seiji; Sakai, Madoka; Mitsugi, Shogo

doi:10.1584/jpestics.15.593

Cited by 13 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The H 2 O‐soluble and NH 4 NO 3 ‐exchangeable fractions are among the more soluble TE fractions in soils and, therefore, simulate those more likely to interact with microrganisms and enzyme molecules in soil. However, while inhibitory effects of organic pollutants on soil arylesterase activity have been characterized (Nakamura et al , 1990; Moon & Smith, 2005), information on inhibitory effects of TE on soil arylesterase activity is scarce. Arylesterase activity cleaves carboxyl groups adjacent to ester bonds and, differently from other hydrolase activities for which metal ions can be important functional cofactors (Coleman, 1992; Bouma et al , 1997; Holm & Sander, 1997), to our knowledge hydrolysis of p‐nitrophenyl acetate by this enzyme activity does not require metal ions (Kaiser et al , 2006; Torres et al , 2009), and variable effects of various TE on arylesterase from in vitro studies have been reported (Kaiser et al , 2006; Fahmy et al , 2008; Torres et al , 2009).…”

Section: Discussionmentioning

confidence: 99%

Arylesterase activity in trace element contaminated soils

Renella

Zornoza

Landi

et al. 2011

European Journal of Soil Science

View full text Add to dashboard Cite

Arylesterase activity was measured along with microbial biomass C (B C ) and respiration, and H 2 O-soluble and NH 4 NO 3 -exchangeable trace elements (TE), in contaminated soils (TECS) from three long-term field trials. The AGIR (AG) soils were contaminated only by inorganic Cd, whereas the Ambarès (AM) and Louis Fargue (LF) soils were contaminated mainly by Mn and Zn, and Cd and Ni, respectively, from past sludge application at different rates. In the AG soils, the arylesterase activity decreased with increasing Cd total concentration and solubility. In the AM soils, the arylesterase activity decreased with increased contamination, whereas in the LF soils the arylesterase activity was increased by sludge additions, with the higher values detected in soils with lower sludge incorporation rates. In the AG and AM soils, arylesterase activity was significantly and negatively correlated with H 2 O-soluble and NH 4 NO 3 -exchangeable TE, whereas in the LF soils it showed a 'hump'-shaped trend with sludge additions and could not be modelled. Multiple regression analysis showed significant interactions between TE, soil pH value and TOC with this enzyme activity. Respiration values of the AG soils generally increased while the B C generally decreased with Cd contamination, while different trends were observed in the AM and LF soils. No trends in the enzyme activity-to-B C ratios were found in relation to soil contamination. Information regarding the inhibition of arylesterase activity in TECS can be important for the management of soils co-contaminated by TE and organic pollutants, in which the detoxification action of arylesterase may be reduced.

show abstract

Section: Discussionmentioning

confidence: 99%

Arylesterase activity in trace element contaminated soils

Renella

Zornoza

Landi

et al. 2011

European Journal of Soil Science

View full text Add to dashboard Cite

show abstract

“…Aqueous layer (0.5ml) was taken to which 0.5ml (1M) sodium hydroxide and 4 ml double distilled water were added. Reading was taken at 400nm and enzyme activity was expressed as μg pNP released g -1 dry soil h -1 [ 33 ] .…”

Section: Methodsmentioning

confidence: 99%

Cultivable and metagenomic approach to study the combined impact of nanogypsum and Pseudomonas taiwanensis on maize plant health and its rhizospheric microbiome

et al. 2021

View full text Add to dashboard Cite

In the present study we examined the effect of nanogypsum and Pseudomonas taiwanensis strain BCRC 17751on plant and soil health using conventional and metagenomics approaches. Soil physicochemical properties and agronomical parameters of maize plants were reported to be better when applied with nanogypsum and bacterial inoculum together. When compared to control a significant increase in total bacterial counts, nitrogen, phosphorus, potassium (NPK) solubilizing bacterial population and soil enzyme activities (fluorescein diacetate, alkaline phosphatase, dehydrogenase, β-glucosidase, arylesterase and amylase) was reported in treatments. The metagenomics studies revealed dominance of beneficial bacteria such as Proteobacteria, Bacteriodetes, Planctomycetes, Acidobacteria and Nitrospirae in treated soil. On the other hand some novel bacterial diversity was also reported in treated soil which was evident from presence of taxonomically unclassified sequences. Hence, it can be concluded that combined application of nanogypsum and Pseudomonas taiwanensis in maize help in improving the structure and function of soil which affects the plant health without causing any toxic effect. However, in situ validation of the prescribed treatment is required under field conditions on different crops in order to give maximum benefits to the farmers and the environment.

show abstract

“…0.5 ml of aqueous layer was taken; 0.5 ml NaOH and ddw (4 ml) were added. Absorbance was taken at 400 nm [ 34 ].…”

Section: Methodsmentioning

confidence: 99%

Impact of nanophos in agriculture to improve functional bacterial community and crop productivity

Chaudhary

Parveen

et al. 2021

BMC Plant Biol

View full text Add to dashboard Cite

Background Since the World’s population is increasing, it’s critical to boost agricultural productivity to meet the rising demand for food and reduce poverty. Fertilizers are widely used in traditional agricultural methods to improve crop yield, but they have a number of negative environmental consequences such as nutrient losses, decrease fertility and polluted water and air. Researchers have been focusing on alternative crop fertilizers mechanisms to address these issues in recent years and nanobiofertilizers have frequently been suggested. “Nanophos” is a biofertilizer and contains phosphate-solubilising bacteria that solubilises insoluble phosphate and makes it available to the plants for improved growth and productivity as well as maintain soil health. This study evaluated the impact of nanophos on the growth and development of maize plants and its rhizospheric microbial community such as NPK solubilising microbes, soil enzyme activities and soil protein under field condition after 20, 40 and 60 days in randomized block design. Results Maize seeds treated with nanophos showed improvement in germination of seeds, plant height, number of leaves, photosynthetic pigments, total sugar and protein level over control. A higher activity of phenol, flavonoid, antioxidant activities and yield were noticed in nanophos treated plants over control. Positive shift in total bacterial count, nitrogen fixing bacteria, phosphate and potassium solubilizers were observed in the presence of nanophos as compared to control. Soil enzyme activities were significantly (P < 0.05) improved in treated soil and showed moderately correlation between treatments estimated using Spearman rank correlation test. Real time PCR and total soil protein content analysis showed enhanced microbial population in nanophos treated soil. Obtained results showed that nanophos improved the soil microbial population and thus improved the plant growth and productivity. Conclusion The study concluded a stimulating effect of nanophos on Zea mays health and productivity and indicates good response towards total bacterial, NPK solubilising bacteria, soil enzymes, soil protein which equally showed positive response towards soil nutrient status. It can be a potential way to boost soil nutrient use efficiency and can be a better alternative to fertilizers used in the agriculture.

show abstract

Enzymological Properties of Three Soil Hydrolases and Effects of Several Pesticides on Their Activities

Cited by 13 publications

References 0 publications

Arylesterase activity in trace element contaminated soils

Arylesterase activity in trace element contaminated soils

Cultivable and metagenomic approach to study the combined impact of nanogypsum and Pseudomonas taiwanensis on maize plant health and its rhizospheric microbiome

Impact of nanophos in agriculture to improve functional bacterial community and crop productivity

Contact Info

Product

Resources

About