Enzymological Characterization of EpoA, a Laccase-Like Phenol Oxidase Produced by Streptomyces griseus

Endo, Kohki; Hayashi, Yutaka; Hibi, T.; Hosono, Kuniaki; Beppu, Teruhiko; Uéda, Kenji

doi:10.1093/jb/mvg086

Cited by 149 publications

(92 citation statements)

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“…35 A collection of references on recombinant expression of laccases in bacterial hosts is available in Table 1. [36][37][38][39][40][41][42][43][44][45][46] Plants have been successfully used as hosts for the recombinant expression of fungal and plant laccases. Expression of secreted recombinant laccases can result, for example, in phytoremediation systems.…”

Section: Laccase Recombinant Expressionmentioning

confidence: 99%

Heterologous laccase production and its role in industrial applications

Piscitelli¹,

Pezzella²,

Giardina³

et al. 2010

Bioengineered Bugs

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114

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Section: Laccase Recombinant Expressionmentioning

confidence: 99%

Heterologous laccase production and its role in industrial applications

Piscitelli¹,

Pezzella²,

Giardina³

et al. 2010

Bioengineered Bugs

149

114

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“…Polyphenolics inhibit decomposition by binding to the reactive site of extracellular enzymes and through the formation of phenolic complexes (Horner et al 1988) in low temperatures (Freeman et al 2001b), oxygen (Pind et al 1994, Freeman et al 2001a) and pH (Ruggiero andRadogna 1984, Pind et al 1994). Thus, activity of phenol oxidases is believed to be a key regulator of peatland C cycling and storage (Freeman et al 2001b(Freeman et al , 2004 and known microbial producers include fungi (Bending and Read 1997) and bacteria (Hullo et al 2001, Endo et al 2003, Fenner et al 2005. However, litter and organic soil phenol oxidase activity was found to be positively correlated with moisture content, which suggests that enzyme activity may require an optimal moisture level and be limited by drought in shallow organic soils (Toberman et al 2008).…”

Section: Impacts On Aerobic Microbial Communitiesmentioning

confidence: 99%

Aerobic carbon-cycle related microbial communities in boreal peatlands: responses to water-level drawdown

Peltoniemi¹

2010

Diss. For.

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Boreal peatlands represent a considerable portion of the global carbon (C) pool. These environments are vulnerable to changes in water level (WL), which can vary dramatically in response to climate or land-use change. Water-level drawdown (WLD) causes peatland drying and induces a vegetation change, which in turn affects the decomposition of soil organic matter and the release of greenhouse gases (CO 2 and CH 4 ) into the atmosphere. The objective of this thesis was to study the microbial communities related to the C cycle and their response to WLD in two boreal peatlands.The first study site (Lakkasuo) is a boreal peatland complex that was partly drained in 1961 to investigate the long-term effects of WLD, and includes three site types with different nutrient levels. At the same location, an experiment simulating the predicted effect of climate change was carried out in 2001 to study the short-term effects of WLD. The second study site (Suonukkasuo) is a boreal fen with a WL gradient caused by a groundwater extraction plant; the undisturbed fen grades into a pine-dominated peatland forest. Microbial communities were studied with phospholipid fatty acid (PLFA) analysis, PCR-DGGE and multivariate analysis.Both sampling depth and site type had a strong impact on all microbial communities. In general, bacteria dominated the deeper layers of the nutrient-rich fen and the wettest surfaces of the nutrient-poor bog sites, whereas fungi seemed more abundant in the drier surfaces of the nutrient-poor bog. WLD clearly affected the microbial communities but the effect was dependent on site type. Fungi and Gram-negative bacteria seemed to benefit and actinobacteria to suffer from the WLD in the fens. The fungal and methane-oxidizing bacteria (MOB) community composition changed at all sites but the actinobacterial community response was apparent only in the nutrientrich fen after WLD.The actinobacterial response to WLD was minor compared to that of the fungal community. The response was greatest in the nutrient-rich fen and least in the nutrient-poor bog. Microbial communities became more similar among sites after long-term WLD. Litter quality had a large impact on community composition, whereas the effects of site type and WLD were relatively minor. The decomposition rate of fresh organic matter was influenced slightly by actinobacteria, but not at all by fungi. Overall, the results were in line with patterns of vegetation change in the study sites.Field respiration measurements in the northern fen indicated that short term WLD accelerates the decomposition of soil organic matter. In addition, a correlation between activity and certain fungal sequences indicated that community composition affects the decomposition of old organic matter in deeper layers of the peat profile. Fungal sequences were matched to taxa capable of utilizing a broad range of substrates. Most of the actinobacterial sequences could not be matched to characterized taxa in reference databases. WLD had a negative impact on CH 4 oxidation, especiall...

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“…Enzymes that belong to this family include laccases, ascorbate oxidases, and ceruloplasmin in eukaryotes. Even though several bacterial MCOs have also been described, the biological roles of MCOs of prokaryotic origins are diverse and apparently unrelated (9,11,15,17,19,20,35,36). Some of them have been implicated in copper tolerance (3,6,23,31).…”

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confidence: 99%

Differential Expression of the Three Multicopper Oxidases from Myxococcus xanthus

et al. 2007

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Myxococcus xanthus is a soil bacterium that undergoes a unique life cycle among the prokaryotes upon starvation, which includes the formation of macroscopic structures, the fruiting bodies, and the differentiation of vegetative rods into coccoid myxospores. This peculiarity offers the opportunity to study the copper response in this bacterium in two different stages. In fact, M. xanthus vegetative rods exhibit 15-fold-greater resistance against copper than developing cells. However, cells preadapted to this metal reach the same levels of resistance during both stages. Analysis of the M. xanthus genome reveals that many of the genes involved in copper resistance are redundant, three of which encode proteins of the multicopper oxidase family (MCO). Each MCO gene exhibits a different expression profile in response to external copper addition. Promoters of cuoA and cuoB respond to Cu(II) ions during growth and development; however, they show a 10-fold-increased copper sensitivity during development. The promoter of cuoC shows copper-independent induction upon starvation, but it is copper up-regulated during growth. Phenotypic analyses of deletion mutants reveal that CuoB is involved in the primary copper-adaptive response; CuoA and CuoC are necessary for the maintenance of copper tolerance; and CuoC is required for normal development. These roles seem to be carried out through cuprous oxidase activity.

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Enzymological Characterization of EpoA, a Laccase-Like Phenol Oxidase Produced by Streptomyces griseus

Cited by 149 publications

References 0 publications

Heterologous laccase production and its role in industrial applications

Heterologous laccase production and its role in industrial applications

Aerobic carbon-cycle related microbial communities in boreal peatlands: responses to water-level drawdown

Differential Expression of the Three Multicopper Oxidases from Myxococcus xanthus

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