Enzymic oxidation of tryptamine derivatives

Blaschko, H.; Philpot, Flora J.

doi:10.1113/jphysiol.1953.sp005010

Cited by 53 publications

(9 citation statements)

References 3 publications

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“…This change resulted in a considerable increase in the intensity and sharpness of staining, in spite of the fact that tryptamine is oxidized at a significantly slower rate by MAO from most sources (Blaschko & Philpot, 1953). The difference may be due to the greater chromophoric intensity conferred by the indole ring on the final reaction product or to the larger molecular weight and size of tryptamine, which would tend to reduce the solubility and thus increase the rate of precipitation of the reaction product of the aldehyde with the hydrazide.…”

Section: Development Of the Methodsmentioning

confidence: 99%

Physiological implications of the histochemical localization of monoamine oxidase

Koelle¹,

Valk²

1954

The Journal of Physiology

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Section: Development Of the Methodsmentioning

confidence: 99%

Physiological implications of the histochemical localization of monoamine oxidase

Koelle¹,

Valk²

1954

The Journal of Physiology

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“…If 5-hydroxytryptamine is a hormone, it is not clear what its hormonal function can be and whether it exerts an effect locally in the gastro-intestinal tract or distally in some other tissue. In its passage into the systemic circulation 5-hydroxytryptamine must necessarily pass through the liver which, being rich in amine oxidase, would be a site of destruction of the substance (Blaschko & Philpot, 1953). It is possible, therefore, that 5-hydroxytryptamine is 'protected' by absorption on to blood platelets and that under certain conditions it is released into the plasma.…”

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confidence: 99%

Release of 5‐hydroxytryptamine (serotonin) from the dog's gastro‐intestinal tract

Toh¹

1954

The Journal of Physiology

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provided (1) for the spontaneous release of a substance similar to 5-hydroxytryptamine from the gastro-intestinal tract, (2) for the specific release of such a substance from the perfused stomach. METHODSDogs which had fasted overmght were anaesthetized with pentobarbitone (Nembutal).Collection of blood. Owing to the fragility of platelets, the following precautions were taken in the collection of blood samples. All glass cannulae and centrifuge tubes were treated with silicone. Each tube contained 0-2 ml. of a 1 % solution of heparin, and 15 mg of 'Versene' (di-sodium salt of ethylene diamine tetraacetic acid) in 0 3 ml. of water to give a final concentration of 0.1 % in 15-16 ml. of blood. In order to separate the plasma the blood samples were centrifuged at 3000 rev/min for 20 min.Arterial blood was collected from a cannulated femoral artery. For the collection of portal blood the following operation was performed. The spleen was removed and a branch of the splenic vein was cannulated. A loose ligature was placed around the portal vein near its entrance into the liver. By pulling on this ligature the portal vein was occluded and portal blood diverted into the cannulated splenic vein.Plasma contains unknown substances which interfere with assays for 5-hydroxytryptamine on the rat's isolated colon. This difficulty was overcome either by dialysing plasma and using the dialysates for the assays or by extracting the plasma with acetone and assaying the acetone extracts.Dialysis of plawma. 5 ml. of plasma was placed in a cellophane bag and dialysed against 10 ml. of a saline solution of the same composition as that used in the assay of serotonin on the rat's * Part of this work was performed at

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“…), nerve-heart preparations loose sensitivity to cardio accelerator action, but remain sensitive to 5-HT (11) (according to Loveland (11), reserpine administered over a period of 2 wk to Mercenaria (Venus) reduces the 5-HT content of ganglia to about one-half); (h) 5-HT excites selected ganglion cells within ganglia (41,42) that have been shown to re lease, upon stimulation, diffusible 5-HT; (i) molluscan kidneys (Helix) convert 5-HT to 5-hydroxy-indoleacetic acid (38) ; and (j) molluscan tissues contain amino-oxidase, the enzyme that destroys 5-HT (10,11,43,44).…”

Section: Floreymentioning

confidence: 99%

Comparative Pharmacology: Neurotropic and Myotropic Compounds

Florey¹

1965

Annu. Rev. Pharmacol.

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This is the first time that the title Comparative Pharmacology appears within the covers of the Annual Review of Pharmacology, The Annual Review of Physiology has made it a practice, since 1951, to include each year a section on Comparative Physiology. It is well to pause and to examine the needs and meaning of the prefix "comparative. " In the Introduction to his important text on comparative physiology, August Puetter (1) wrote in 1911,The knowledge of scientifi c laws, as sought by physiology, is reached by a process of abstraction of the common from the abundance of single phenomena. The activity which leads to the cognition of the common (general), is that of comparing and so every scientific work in the field of physiology and every work which transgresses the pure empirical establishing of single facts, is an effort in the sense of " comparative" Physiology. The comparing is the most essential method which we use in science. Does it, under these circumstances, make any sense at all to speak of "Comparative Physiology?" Are not .. . physiology and comparative physiology the same?In the famous eleventh edition of the Encyclopedia Britannica, Ralph Stockman (2) investigates the scope of pharmacology in various countries and comes to the conclusion that,In English speaking countries and [with] the majority of German writers, [pharma cology is] the study of the action of chemical substances (as apart from foods) on all kinds of animals, from bacteria up to man; it is, in fact, a comparative study of the action of chemical bodies on invertebrate and vertebrate animals.

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Enzymic oxidation of tryptamine derivatives

Cited by 53 publications

References 3 publications

Physiological implications of the histochemical localization of monoamine oxidase

Physiological implications of the histochemical localization of monoamine oxidase

Release of 5‐hydroxytryptamine (serotonin) from the dog's gastro‐intestinal tract

Comparative Pharmacology: Neurotropic and Myotropic Compounds

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