Enzymic fluorometric continuous-flow assays for blood glucose, lactate, pyruvate, alanine, glycerol, and 3-hydroxybutyrate.

Lloyd, Barbara; Burrin, J. M.; Smythe, P.; Alberti, K. G. M. M.

doi:10.1093/clinchem/24.10.1724

Cited by 563 publications

(117 citation statements)

References 9 publications

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“…Whole-blood glucose was measured immediately (Glucose Analyzer, Yellow Springs Instruments, Yellow Springs, OH, USA). Whole-blood 3-hydroxybutyrate, glycerol, alanine, pyruvate and lactate were assayed by an automated fluorometric method (Lloyd et al, 1978). Plasma free fatty acids (FFA) were measured by enzymatic centrifugal analysis (Knor et al, 1984).…”

Section: Methodsmentioning

confidence: 99%

Metabolic and Hormonal Responses to Exogenous Hyperthermia in Man

Møller

Beckwith

Butler

et al. 1989

Clinical Endocrinology

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To study whether hyperthermia reproduces hormonal and metabolic responses seen in stress states such as mild infections, six normal male subjects underwent (i) a 3-h hot bath and (ii) a 3-h thermoneutral control period. During the hot bath body temperature rose by 1.2 +/- 0.03 degrees C (mean +/- SEM) and significant peaks of circulating growth hormone (56 +/- 9 vs 7 +/- 4 mU/l), adrenaline (310 +/- 34 vs 152 +/- 39 pmol/l), glucagon (19.2 +/- 4.3 vs 11.8 +/- 2.3 pmol/l) and cortisol were recorded together with slight hyperinsulinaemia (6.5 +/- 1.3 vs 5.3 +/- 1.0 mU/l, P less than 0.05). Hyperthermia was also accompanied by significant increases in circulating levels of free fatty acids (0.93 +/- 0.1 vs 0.46 +/- 0.1 mmol/l), 3-hydroxybutyrate (196 +/- 67 vs 50 +/- 18 mumol/l), glycerol (102 +/- 10 vs 48 +/- 5 mumol/l) and lactate. Blood alanine decreased and blood glucose remained constant. When an intravenous glucose tolerance test was performed during the last hour of hyperthermia signs of impaired first phase and enhanced second phase insulin responses were recorded. Calculated values for glucose disappearance also deteriorated (1.34 +/- 0.19 vs 2.04 +/- 0.50 %/min, P less than 0.05). We conclude that hyperthermia mimics most major metabolic and hormonal changes observed during mild infection and could provide a model to study these conditions.

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Section: Methodsmentioning

confidence: 99%

Metabolic and Hormonal Responses to Exogenous Hyperthermia in Man

Møller

Beckwith

Butler

et al. 1989

Clinical Endocrinology

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“…For the measurement of metabolite concentrations (glucose, glycerol, lactate and pyruvate) 3 ml of whole blood was placed in tubes containing 3 ml 1 M perchloric acid, mixed and centrifuged, and the deproteinised supernatant snap frozen and stored at -80°C until analysis (Lloyd et al, 1978). The remaining blood was centrifuged, the plasma separated, snap frozen where necessary, and stored at -80°C.…”

Section: Methodsmentioning

confidence: 99%

The effects of acute or chronic ingestion of propranolol or metoprolol on the metabolic and hormonal responses to prolonged, submaximal exercise in hypertensive men.

Macdonald¹,

Bennett²,

Brown³

et al. 1984

Brit J Clinical Pharma

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We have studied the effects of single oral doses of, and of 28 days treatment with, placebo, propranolol or metoprolol, on the metabolic and hormonal responses to prolonged exercise in hypertensive men. Blood glucose levels fell during exercise on all occasions. No additional effects of the beta‐adrenoceptor antagonists, compared to placebo, were observed. The exercise‐induced increase in plasma potassium was enhanced after a single dose of propranolol or metoprolol, and also after chronic treatment with propranolol. Chronic treatment with either drug led to an increase in plasma potassium levels at rest. The growth hormone response to exercise was potentiated by a single dose of metoprolol or propranolol, and after chronic treatment with the drugs. A single dose of propranolol (but not metoprolol) was associated with a marked increase in plasma cortisol and adrenaline levels during exercise. After chronic treatment no such increase occurred. In both the acute and chronic phases of the study, blood lactate levels were higher during exercise in the presence of either propranolol or metoprolol compared to placebo, whereas non‐esterified fatty acid levels were lower. A single dose of metoprolol produced a significantly greater reduction in blood glycerol levels during exercise than a single dose of propranolol. After chronic treatment, both propranolol and metoprolol produced similar reductions in blood glycerol levels during exercise. After a single dose, both drugs significantly augmented the increase in plasma noradrenaline levels during exercise. A similar effect was seen after chronic treatment.

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“…Blood glucose, lactate, pyruvate, alanine and 3-hydroxybutyrate were measured by automated enzymatic fluorimetric techniques (Lloyd et al, 1978) and blood acetoacetate by a manual spectrophotometric method (Price et af., 1977). Serum NEFA were measured by a radioactive cobalt method (Ho & Meng, 1969), and serum insulin (Soeldner & Slone, 1965) and growth hormone (Boden & Soeldner, 1967) by double antibody radioimmunoassays.…”

Section: Methodsmentioning

confidence: 99%

Dopaminergic Control of Ketogenesis in Fasting

Blesa-Malpica

Johnston

Burrin

et al. 1981

Clinical Endocrinology

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The role of dopamine in starvation ketonaemia was investigated in male Wistar rats by administration of a specific dopamine receptor antagonist, metoclopramide (4 mg . kg-1 . 24h-1), or placebo, intragastrically during a 48-h fast. Starvation alone caused a fall in blood glucose and gluconeogenic precursor concentrations, which was unaffected by metoclopramide administration. Circulating 3-hydroxybutyrate and acetoacetate levels rose with fasting alone but metoclopramide impaired this ketonaemic response. After 48-h starvation, total ketone body concentrations (mean +/- SEM) were 2.28 +/- 0.19 mmol/l with metoclopramide therapy, 3.49 +/- 0.21 mmol/l with placebo, P less than 0.001. Plasma non-esterified fatty acid levels were similar in metoclopramide- and placebo-treated animals, as were circulating concentrations of insulin, glucagon and growth hormone. Metoclopramide thus decreased the ketonaemic response to starvation without an apparent change in lipolysis or circulating hormone levels, suggesting a direct role for dopamine in production of starvation ketonaemia.

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Enzymic fluorometric continuous-flow assays for blood glucose, lactate, pyruvate, alanine, glycerol, and 3-hydroxybutyrate.

Cited by 563 publications

References 9 publications

Metabolic and Hormonal Responses to Exogenous Hyperthermia in Man

Metabolic and Hormonal Responses to Exogenous Hyperthermia in Man

The effects of acute or chronic ingestion of propranolol or metoprolol on the metabolic and hormonal responses to prolonged, submaximal exercise in hypertensive men.

Dopaminergic Control of Ketogenesis in Fasting

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