Enzymic degradation of deoxyribose derivates for determination of isotope

Wright, Elmer M.; Sable, Henry Z.

doi:10.1016/0006-3002(59)90560-8

Cited by 5 publications

(5 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Degradations. The carbohydrates were degraded to determine the distribution of C14 using the following methods: glucose was fermented with Leuconostoc mesenteroides, according to the method of Bernstein and Wood (1957); ribose was fermented with Lactobacillus plantarum, according to Bernstein (1953); and deoxyribose, according to Wright and Sable (1959). In these degradations the acetic acid was always degraded by the method of Phares (1951).…”

mentioning

confidence: 99%

BIOSYNTHESIS OF PENTOSES IN ESCHERICHIA COLI

1961

Self Cite

View full text Add to dashboard Cite

Previous studies have shown that Escherichia coli possesses two principal pathways for the biosynthesis of pentose from hexose. The best known involves the decarboxylation of 6-phosphogluconate to ribulose 5-phosphate. This is the principal pathway in E. coli grown on glucose (Lanning and Cohen, 1954; Bernstein, 1956). This pathway will be referred to as the oxidative pathway. (The term oxidative pathway is used here to describe the formation of pentose by the oxidative decarboxylation of 6-phosphogluconate and excludes any resynthesis by a complete pentose cycle. The stoichiometry is: 3 hexose + oxygen 3 pentose + 3 CO2. The term transketolasetransaldolase pathway is used here to indicate nonoxidative synthesis of pentose from hexose,

show abstract

mentioning

confidence: 99%

BIOSYNTHESIS OF PENTOSES IN ESCHERICHIA COLI

1961

Self Cite

View full text Add to dashboard Cite

show abstract

“…Studies of biosynthesis of carbohydrates by normal and bacteriophage-infected Escherichia coli have been undertaken in this laboratory. Some of the metabolic properties of these systems have been reported previously (Bagatell et al, 1958(Bagatell et al, , 1959Wright and Sable, 1959). It had appeared from the work of other authors (Lanning and Cohen, 1954;Bernstein, 1956) that E. coli growing on glucose synthesize pentose largely by oxidative decarboxylation of hexose monophosphate.…”

mentioning

confidence: 86%

“…For radioactivity determinations the samples were converted to CO2 either by total oxidation (Van Slyke and Folch, 1940) or by specific degradation (Wright and Sable, 1959) and then plated as BaCO3 on filter paper discs. Counting was done in a Tracerlab windowless gas-flow counter, with a statistical counting error of 2 per cent or less.…”

mentioning

confidence: 99%

GROWTH OF ESCHERICHIA COLI AND PRODUCTION OF BACTERIOPHAGE IN DEFINED MEDIA

et al. 1960

Self Cite

View full text Add to dashboard Cite

“…The radioactive purine nucleotides were diluted with carrier nucleotide and were converted to deoxyribose 5-phosphate by hydrolysis in dilute H2SO4 as described by Wright and Sable (1959). Glucose, which occurs in the DNA of T-even bacteriophages as the glucoside of hydroxymethyl cytosine nucleotide (Volkin, 1954;Jesaitis, 1957;Loeb and Cohen, 1959) was isolated in the following manner.…”

mentioning

confidence: 99%

“…Determination of isotope distribution. The glucose was degraded by fermentation with Leuconostoc mesenteroides according to Bernstein and Wood (1957), except that the acetic acid was degraded by the method of Phares (1951); labeling of deoxyribose was determined by degradation of deoxyribose 5-phosphate according to Wright and Sable (1959). The radioactivity was measured in two ways.…”

mentioning

confidence: 99%

BIOSYNTHESIS OF PENTOSE IN ESCHERICHIA COLI

1961

Self Cite

View full text Add to dashboard Cite

The biosynthesis of ribose and deoxyribose in Escherichia coli involves both the oxidative pathway through 6-phosphogluconate and the nonoxidative pathway through the transketolase and transaldolase sequence (Lanning and Cohen, 1954; Bernstein, 1956; Bagatell, Wright, and Sable, 1959). The proportions of the oxidative and nonoxidative pathways depend on the nature of the principal carbon source in the medium, on the stage of growth of the culture, and on the previous history of the culture (Szynkiewicz, Sable, and Pflueger, 1961). In addition evidence has been presented that ribose and deoxyribose have a common origin in this organism (Bernstein and Sweet, 1958; Bagatell et al., 1959). Two studies (Lanning and Cohen, 1955; Loeb and Cohen, 1959) suggest that in bacteriophage-infected E. coli the nonoxidative pathway becomes of greater importance. The studies reported here support the view that both pathways are of considerable importance in the biosynthesis of deoxyribose in the presence of bacteriophage infection. Racker (1952) found an aldolase in E. coli that catalyzes the reversible reaction: CH2OPO3H2-CHOH-CHO + CH3-CHO = CH2OPO3H2-CHOH-CHOH-CH2-CHO and it has been considered that this reaction or a similar type of aldol condensation of 3-carbon and 2-carbon precursors might be an important mechanism in the biosynthesis of deoxyribose. Evidence is presented here that the biosynthesis does not occur by such an aldol condensation. MATERIALS AND METHODS E. coli strain R-2 and bacteriophage strain T2H were maintained and cultured as described

show abstract

Enzymic degradation of deoxyribose derivates for determination of isotope

Cited by 5 publications

References 13 publications

BIOSYNTHESIS OF PENTOSES IN ESCHERICHIA COLI

BIOSYNTHESIS OF PENTOSES IN ESCHERICHIA COLI

GROWTH OF ESCHERICHIA COLI AND PRODUCTION OF BACTERIOPHAGE IN DEFINED MEDIA

BIOSYNTHESIS OF PENTOSE IN ESCHERICHIA COLI

Contact Info

Product

Resources

About