Enzymic and structural studies on processed proteins from the vacuolar (lutoid-body) fraction of latex of Hevea brasiliensis

Subroto, Toto; Vries, Henk de; Schuringa, Jan Jacob; Soedjanaatmadja, Ukun M.S.; Hofsteenge, Jan; Jekel, Peter A.; Beintema, Jaap J.

doi:10.1016/s0981-9428(01)01325-0

Cited by 24 publications

(31 citation statements)

References 25 publications

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“…A 43 kDa protein that is partially homologue to patatin, the main storage protein in potato, was also found in the bottom fraction and serum [10,12]. The amount of this protein is 1 %-w of the bottom fraction [10].…”

Section: Latex Yield and Propertiesmentioning

confidence: 93%

“…A previous study showed that most of the hevein from the latex is conserved after isolation from rubber factory effluent, obtaining a concentration of 0.7 g/l and suggesting that the effluent can be a source of proteins with antifungal properties [39]. Other proteins that are identified in the bottom fraction are 1,3-bglucanase and hevamine; the latter shows high chitinase/ lysozyme activity [11,12].…”

Section: Latex Yield and Propertiesmentioning

confidence: 99%

“…The majority (50-70 %) of proteins in this fraction consists of hevein [12,15], a 5 kDa protein that contains 18 % cysteine and is soluble in the presence of neutral salts [7,12,13]. The allergenic and antifungal properties of hevein are well identified [7,8].…”

Section: Latex Yield and Propertiesmentioning

confidence: 99%

“…According to our current knowledge, the protein with the most prospective application is hevein for antimicrobial or antifungal agents [7,70]. The other protein with potential application is the 43 kDa patatin-homologue [10,12], due to its similarity with patatin. Patatin is currently investigated for food application as emulsifier, gelling agent, and foaming agent [71][72][73][74], and synthesis of monoacylglycerols [75].…”

Section: Latexmentioning

confidence: 99%

“…Protein, being one of the main constituents of biomass and the most valuable part in some cases, can potentially increase the overall economics of rubber plantations. The proteins in rubber tree latex are well-identified [7][8][9][10][11][12][13][14][15], although some are characterised for their allergenic properties [8,9,15]. Less attention is given on proteins in the seeds and the leaves of rubber tree [16,17].…”

Section: Introductionmentioning

confidence: 99%

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Valorisation of Proteins from Rubber Tree

WIDYARANI

Coulen

Sanders

et al. 2016

Waste Biomass Valor

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Purpose The objective of this study was to identify the availability, possible applications, and economic potential of proteins that are present in different parts of the rubber tree. Proteins from non-food sources can be used in e.g. animal feed or biochemicals production with no or little competition with food production, rendering them important biobased feedstock. Rubber tree is primarily grown for its latex that is used in rubber production. Indonesia has the largest rubber plantation area that is mostly owned and run by smallholder farmers. Using non-latex fractions from the rubber tree may generate additional income, and increase the economics of rubber plantations in general. Methods Several biomass streams from the rubber tree and subsequent latex processing were considered. Data were compiled from literature, a case study, and interviews with researchers, smallholder farmers, and managers at rubber processing plant and plantation. Results Latex waste streams, seeds, and leaves were considered to have the highest potential based on the amount of available proteins, and processes to isolate proteins from these streams were proposed. Isolation of specific functional properties from natural sources requires complex (and expensive) separation processes and therefore only economically feasible when specific use of the protein(s) for high value applications can be identified. Purification of many interesting proteins from latex fractions has already been described. Processing of seeds and leaves may also yield useful proteins for food, other purposes, and also still unknown high value applications. Conclusions A biorefinery concept can be applied to obtain multiple products from the seeds and leaves, and protein extraction can be performed with available knowledge and technology. Small scale processing can be more beneficial for the farmers, especially if the products are used locally for feed.

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Section: Latex Yield and Propertiesmentioning

confidence: 93%

Section: Latex Yield and Propertiesmentioning

confidence: 99%

Section: Latex Yield and Propertiesmentioning

confidence: 99%

Section: Latexmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Valorisation of Proteins from Rubber Tree

WIDYARANI

Coulen

Sanders

et al. 2016

Waste Biomass Valor

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Characterization of a cDNA coding for an extracellular calmodulin-binding protein from suspension-cultured cells of Angelica dahurica

Guohong

Hou

Ding

et al. 2005

Planta

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In order to characterize a specific extracellular 21-kDa calmodulin-binding protein (named: ECBP21) from Angelica dahurica L. suspension-cultured cells, the cDNA coding for the protein has been cloned. Here, Southern blot analysis shows that there are at least two copies of ECBP21 gene in Angelica genome. Using truncated versions of ECBP21 and synthetic peptide in CaM binding assays, we mapped the calmodulin-binding domain to a 16-amino acid stretch (residues 200-215) at the C-terminal region. The ECBP21 was localized in the cell wall area by the immunogold electron microscopy and by GFP labeling method. These results define ECBP21 as a kind of an extracellular calmodulin-binding protein (CaMBP). Furthermore, using Northern blot analysis, we examined the expression dynamics of ecbp21 during the incubation of Angelica suspension-cultured cells and the treatments with some growth regulators. The above studies further provide the molecular evidence for the existence of the gene coding for extracellular CaMBPs and imply a possible role for ECBP21.

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Hevea brasiliensis latex proteomics: a review of analytical methods and the way forward

Habib

Ismail

2020

J Plant Res

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Enzymic and structural studies on processed proteins from the vacuolar (lutoid-body) fraction of latex of Hevea brasiliensis

Cited by 24 publications

References 25 publications

Valorisation of Proteins from Rubber Tree

Valorisation of Proteins from Rubber Tree

Characterization of a cDNA coding for an extracellular calmodulin-binding protein from suspension-cultured cells of Angelica dahurica

Hevea brasiliensis latex proteomics: a review of analytical methods and the way forward

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