Enzymes Responsible for Acetate Oxidation by Acetic Acid Bacteria

Saeki, Akihiko; Matsushita, Kazunobu; Takeno, Sachio; Taniguchi, Mariko; Toyama, Hirohide; Theeragool, Gunjana; Lotong, Napha; Adachi, Osao

doi:10.1271/bbb.63.2102

Cited by 30 publications

(30 citation statements)

References 35 publications

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“…All operations were done at 4 mC. Bacteria were grown to lateexponential phase, harvested, washed twice with ice-cold 0n1 M Tris\HCl buffer (pH 7n4) containing 10 mM MgSO % , 10 % glycerol and 5 mM β-mercaptoethanol (Saeki et al, 1999), and resuspended in the same buffer. After cell disruption by sonication, cell debris was removed by centrifugation for 30 min at 6000 g. Part of the supernatant was used as the cellfree extract ; the other part was used to obtain the membrane fraction.…”

Section: Methodsmentioning

confidence: 99%

“…Catalytic activity was calculated from the U. Kretzschmar and others decrease in absorbance of NADH at 340 nm, using an absorption coefficient of 6n22 cm − " mM − " (Horecker & Kornberg, 1948). Pyruvate carboxylase (PC) and PEP carboxylase (PEP-C) activities were measured according to Saeki et al (1999) by the decrease in absorbance of NADH at 340 nm in a coupled test with MDH. The reaction was started by the addition of pyruvate or phosphoenolpyruvate (PEP).…”

Section: Methodsmentioning

confidence: 99%

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Malate:quinone oxidoreductase is essential for growth on ethanol or acetate in Pseudomonas aeruginosa The GenBank accession number for the Pseudomonas aeruginosa ATCC 17933 mqo sequence reported in this work is AY129296.

et al. 2002

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Pseudomonas aeruginosa ATCC 17933 growing aerobically on ethanol uses a pyrroloquinoline quinone-dependent ethanol oxidation system. A mutant with an interrupted putative mqo gene, in which malate :quinone oxidoreductase (MQO), an enzyme involved in the citric acid cycle/glyoxylate cycle, was defective, showed a severe growth defect on ethanol and was unable to grow on acetate. Glucose, lactate, succinate or malate supported growth of the mutant. However, an NAD-dependent malate dehydrogenase activity could not be detected. Complementation of the mutant by the wild-type allele of the mqo gene restored wild-type behaviour. The wild-type expressed the dyedependent MQO and NAD(P)-dependent malic enzymes (MEs). Pyruvate carboxylase (PC) was found upon growth of the wild-type and the mutant on all substrates studied. PC activity in the wild-type was induced on glucose and lactate and was always higher on all substrates in the mqo mutant. In P. aeruginosa ATCC 17933, an active MQO is required for growth on ethanol or acetate, while with glucose, lactate, succinate or malate an apparent bypass route operates, with MEs using malate for generating pyruvate, which is carboxylated to oxaloacetate by PC. To the authors' knowledge, this is the first time that a specific mutant MQO phenotype has been observed, caused by the inactivation of a gene encoding MQO activity. mqo of P. aeruginosa ATCC 17933 corresponds to mqoB (PA4640) of the P. aeruginosa PAO1 genome project.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Malate:quinone oxidoreductase is essential for growth on ethanol or acetate in Pseudomonas aeruginosa The GenBank accession number for the Pseudomonas aeruginosa ATCC 17933 mqo sequence reported in this work is AY129296.

et al. 2002

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“…Chinnawirotpisan et al (2003) and Saeki et al (1997Saeki et al ( , 1999 justified this biochemical phenomenon as follow: Acetobacter strains always show three growth characteristics in alcohol media. The first one is the alcohol oxidation into acetic acid, the second is a no growth step and the last one is the overoxidation of accumulated acetic acid.…”

Section: Resultsmentioning

confidence: 92%

Isolation of thermotolerant and high acetic acid-producing Acetobacter pasteurianus from Ivorian palm wine

Konate

Akpa

Koffi

et al. 2014

Emir. J. Food Agric

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The aim of this work was to identify acetic acid bacteria expressing technological characteristics for further use in vinegar production in tropical countries. It was focused on isolation and identification of thermotolerant acetic acid bacteria strains from Elaeis guineensis wine of Côte d'Ivoire (Ivorian palm wine). A screening was performed to find out strains with high vinegar production, tolerant against high ethanol, acid and sugar concentrations as well as tolerating high production temperatures. Among 104 isolated strains, 5 were selected for their growth ability, acetification capacity (acetic acid production higher than 30 g/l) and suroxydation at 37°C. Tolerance against 6% acetic acid and 9% ethanol was observed. Osmotolerance study showed tolerance against 5% and 10% glucose giving 100% and 50% relative growth, respectively. Resistance against desiccation showed survival rate of 50% at 37°C after 5 h treatment and 10% after 20 h treatment. The best aeration rate in flasks for acetification was 70%. Polyphasic identification study based on biochemical, physiological and molecular characterization showed that the 5 isolates were all Acetobacter pasteurianus. Owing to their potentialities, these strains may be used as starters in vinegar production after conducting preservation studies.

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“…6) The enzymes working in the TCA cycle are related to acetate overoxidation, together with acetyl-CoA synthase and phosphoenolpyruvate carboxylase, 7,8) but the mechanism responsible for acetic acid resistance cannot be explained only by acetate assimilation, because Acetobacter and Gluconacetobacter species survive without assimilation of acetate under high concentrations of acetic acid under fermentation conditions. 1,9) Acetobacter species have been found to have proton motive force-dependent and ABC-transporter-like efflux pump systems for acetic acid in Acetobacter pasteurianus IFO3283 10) and Acetobacter aceti 11) respectively.…”

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confidence: 99%

Acetic Acid Fermentation ofAcetobacter pasteurianus: Relationship between Acetic Acid Resistance and Pellicle Polysaccharide Formation

Kanchanarach

Theeragool

Inoue

et al. 2010

Bioscience, Biotechnology, and Biochemistry

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Enzymes Responsible for Acetate Oxidation by Acetic Acid Bacteria

Cited by 30 publications

References 35 publications

Malate:quinone oxidoreductase is essential for growth on ethanol or acetate in Pseudomonas aeruginosa The GenBank accession number for the Pseudomonas aeruginosa ATCC 17933 mqo sequence reported in this work is AY129296.

Malate:quinone oxidoreductase is essential for growth on ethanol or acetate in Pseudomonas aeruginosa The GenBank accession number for the Pseudomonas aeruginosa ATCC 17933 mqo sequence reported in this work is AY129296.

Isolation of thermotolerant and high acetic acid-producing Acetobacter pasteurianus from Ivorian palm wine

Acetic Acid Fermentation ofAcetobacter pasteurianus: Relationship between Acetic Acid Resistance and Pellicle Polysaccharide Formation

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