Enzymes of strains of pleurotus species (Basidiomycetes) compared by electrophoresis.

Magae, Yumi; Raga, Keiko; Taniguchi, Hajime; Sasaki, Takashi

doi:10.2323/jgam.36.69

Cited by 12 publications

(11 citation statements)

References 19 publications

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“…Isozyme analysis is also an efficient method for determining variation in fungi (Micales, Bonde & Peterson, 1986) and has been widely used in the identification of species and isolates and population structures of a large number of genera including Neurospora (Spieth, 1975), Heterobasidium (Stenlid, 1985;Orotsina et al, 1993), Pleurotus (Magae et al, 1990), Phytophthora (Oudeman & CofTey, 1991), Lentinus (Ohmasa & Furukawa, 1986), Schizophyllum (Lily & Charvat, 1987), Tuber (Dupre et al, 1993) and several ecto and endomycorrhizal fungi (Sen & Hepper, 1986;Sen, 1990;Keller, 1992). Isozyme patterns has already been used to characterize Armillaria species both in Europe (Wahlstrom et al, 1991) and in North America (Morrison et al, 1985;Lin, Dumas & Hubbes, 1989).…”

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Discrimination of some African Armillaria species by isozyme electrophoretic analysis

Agustian¹,

Mohammed

Guillaumin

et al. 1994

New Phytologist

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SUMMARYTen Armillaria isolates, collected from various host plants and widespread geographical origins in tropical Africa, were cultivated on orange fragments in the presence of water and two different culture media in order to optimize enzyme and mycelial cord production. Seven enzymes involved in the primary metabolism of nitrogen and carbon (glutamate dehydrogenases, aspartate aminotransferase, malate dehydrogenase, glucose 6-phosphate dehydrogenase, 6-phospbogluconatt dehydrogenase and isocitrate dehydrogenase) were extracted from the mycelial cords and analyzed by polyacrylamide gel electrophoresis. Cluster analy.sis based on calculated similarity i-alues derived from isozyme banding patterns separated the isolates into fi\'e groups. Two isolates considered as belonging to A. metlea ssp. africana (an African species closely related to European A. metlea) were present in a clearly separated cluster when compared to the other isolate groups. Two Kenyan isolates, belonging to an as yet unnamed biological species, which w ere characterized by the production of few slow growing mycelial cords, were also found in a separate cluster with slightly greater similarit\' coefficients to the other isolates. The six other isolates, referred to as isolates of A. heimii (a highly variable species with different sexual systems) fell into three sub^clusters of variable homology. The two bomothallic heimii isolates from Tanzania and Malawi, which were \ery closely related, displaying 100 "" isozyme similarity, exhibited a higher degree of similarit>' with the two other homothalhc heimii isolates from Zimbabwe and Congo, than with the two heterothailic unifactorial heimti isolates from Cameroon and Gabon. The value of isozymes in the classification of African Armtllaria spp. is discussed.

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Discrimination of some African Armillaria species by isozyme electrophoretic analysis

Agustian¹,

Mohammed

Guillaumin

et al. 1994

New Phytologist

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“…protein kinase C (PKC) is activated by diacylglycerol, and also by a tumour-promoting phorbol ester phorbol-12-myristate 13-acetate (PMA) (Costagna et a/., 1982), this enzyme has attracted much interest. There have also been numerous reports documenting the involvement of PKC in cellular responses, including cell proliferation and differentiation in mammalian cells (reviewed by Nishizuka, 1986;Fargo & Nishizuka, 1990).…”

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Purification and characterization of protein kinase C from Pleurotus ostreatus

Magae¹

1993

Journal of General Microbiology

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Protein kinase C (PKC) from Pleurotus ostveatus mycelium (Basidiomycotina) was purified by affinity chromatography using activated agarose (Affigel 15) coupled with the protein kinase inhibitor N-(2-aminoethyl)-5-isoquinolinesulphonamide hydrochloride. Using buffer containing 2-0 M-arginine. PKC activity eluted as a single peak corresponding to a protein with apparent molecular mass of approximately 68000 Da as estimated by SDS-PAGE. The enzyme was not stimulated by CaZ+ alone, and activation by phosphatidylserine (PS) was significant only at lop6 M-Ca2+. However, the activity was enhanced by the addition of 1,2-dioctanoyl-sn-glycerol in the presence of PS and Ca2+. Additionally, although sensitivity was lower than for mammalian PKC, the enzyme was activated by a tumour-promoting phorbol ester, phorbol-12-myristate 13-acetate (PMA). The saturation concentration of PMA was 50 pg ml-', which was 5 x lo3 times the value for mammalian PKC.

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“…Isozyme analysis is rather efficient for determining variation in fungi (Micales et al 1986, Bonde et al 1993 and it has been widely used in the identification of species and isolates of a large number of Homobasidiomyceteous genera including Heterobasidion (Stenlid 1985), Pleurotus (Magae et a!. 1990;Zervakis & Labarere 1992), Lentinus (Ohmasa & Furukawa 1986), Armillaria (Wahlstrom et al 1991;Agustian et al 1994) and Clavicorona (Wu et al 1995).…”

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