Enzymes of glycogen metabolism in cerebral cortex of normal and diabetic rats

Prasannan, K. G.; Subrahmanyam, K

doi:10.1111/j.1471-4159.1968.tb06843.x

Cited by 8 publications

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References 10 publications

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“…Altered brain glycogen metabolism has also been observed in diabetes. An in vitro study found the activity of hexokinase (engaging glucose in glycolysis) reduced whereas those of glycogen phosphorylase and synthase increased in cortical slices of diabetic rats, suggesting glycogen to be a more important fuel than glucose for oxidation in this case [52]. Increased activity of glycogen synthase has also been found in the cortex of type 1 diabetic rats, however without any concentration alterations [53].…”

Section: Brain Glycogen and Energetic Dysfunction In Diseasementioning

confidence: 99%

Technical and experimental features of Magnetic Resonance Spectroscopy of brain glycogen metabolism

Soares

Gruetter

Lei

2017

Analytical Biochemistry

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a b s t r a c tIn the brain, glycogen is a source of glucose not only in emergency situations but also during normal brain activity. Altered brain glycogen metabolism is associated with energetic dysregulation in pathological conditions, such as diabetes or epilepsy. Both in humans and animals, brain glycogen levels have been assessed non-invasively by Carbon-13 Magnetic Resonance Spectroscopy ( 13 C-MRS) in vivo. With this approach, glycogen synthesis and degradation may be followed in real time, thereby providing valuable insights into brain glycogen dynamics. However, compared to the liver and muscle, where glycogen is abundant, the sensitivity for detection of brain glycogen by 13 C-MRS is inherently low. In this review we focus on strategies used to optimize the sensitivity for 13 C-MRS detection of glycogen. Namely, we explore several technical perspectives, such as magnetic field strength, field homogeneity, coil design, decoupling, and localization methods. Furthermore, we also address basic principles underlying the use of 13 C-labeled precursors to enhance the detectable glycogen signal, emphasizing specific experimental aspects relevant for obtaining kinetic information on brain glycogen.

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Section: Brain Glycogen and Energetic Dysfunction In Diseasementioning

confidence: 99%