Enzymes mediating resistance to chlorpyriphos in Aphis fabae (Homoptera: Aphididae)

Mirza, Farideh Khan; Yarahmadi, Fatemeh; Jalal-Abadi, Amin Lotfi; Meraaten, Ali Akbar

doi:10.1016/j.ecoenv.2020.111335

Cited by 9 publications

(6 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This motif is represented by the sequence (FVA)- 2)-(DE)-Q (where X represents any amino acid). 32 It is located in the middle of the C-terminal domain and is associated with the glucose moiety. Important residues involved in sugar binding, such as S308 (S306 on the alignment of Figure S1C), W356 (W342), Q359 (Q345), and E382 (E368), as well as residues involved in glucuronic acid interactions, D398 (D384) and Q399 (Q385), are conserved in insect UGTs.…”

Section: ■ Resultsmentioning

confidence: 99%

“…The UGT motif signature consists of conserved sequences, also known as the plant secondary product glycosyltransferase (PSPG) motif, in plants. This motif is represented by the sequence (FVA)-(LIVMF)-(TS)-(HQ)-(SGAC)-G-X(2)-(STG)-X(2)-(DE)X(6)-P-(LIVMFA)-(LIVMFA)-X(2)-P-(LMVFIQ)-X(2)-(DE)-Q (where X represents any amino acid) . It is located in the middle of the C-terminal domain and is associated with the glucose moiety.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Uridine Diphosphate-Glycosyltransferase RpUGT344D38 Contributes to λ-Cyhalothrin Resistance in Rhopalosiphum padi

Liu,

Wang,

Tang

et al. 2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

Uridine diphosphate-glycosyltransferase (UGT) is a key phase II enzyme in the insect detoxification system. Pyrethroids are commonly used to control the destructive wheat aphid Rhopalosiphum padi. In this study, we found a highly expressed UGT gene, RpUGT344D38, in both λ-cyhalothrin (LCR)- and bifenthrin (BTR)-resistant strains of R. padi. After exposure to λ-cyhalothrin and bifenthrin, the expression levels of RpUGT344D38 were significantly increased in the resistant strains. Knockdown of RpUGT344D38 did not affect the resistance of BTR, but it did significantly increase the susceptibility of LCR aphids to λ-cyhalothrin. Molecular docking analysis demonstrated that RpUGT344D38 had a stable binding interaction with both bifenthrin and λ-cyhalothrin. The recombinant RpUGT344D38 was able to metabolize 50% of λ-cyhalothrin. This study provides a comprehensive analysis of the role of RpUGT344D38 in the resistance of R. padi to bifenthrin and λ-cyhalothrin, contributing to a better understanding of aphid resistance to pyrethroids.

show abstract

Section: ■ Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Uridine Diphosphate-Glycosyltransferase RpUGT344D38 Contributes to λ-Cyhalothrin Resistance in Rhopalosiphum padi

Liu,

Wang,

Tang

et al. 2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Neonicotinoids, pyrethroids, organophosphates, and macrolides are important groups of synthetic insecticides that are widely used to control arthropod pests [15]. Insecticide resistance has been reported for various aphid species, including Myzus persicae [16,17], Aphis fabae [18], and Aphis gossypii [19]. Previous studies confirmed that field populations of two wheat aphid species, R. padi and S. avenae, have evolved varying levels of resistance to insecticides with diverse modes of action [20,21].…”

Section: Introductionmentioning

confidence: 98%

The Sensitivity of Field Populations of Metopolophium dirhodum (Walker) (Hemiptera: Aphididae) to Seven Insecticides in Northern China

Gong

Wang

et al. 2021

Agronomy

View full text Add to dashboard Cite

Insect pests are primarily controlled by insecticides. However, the sensitivity decreases and insecticide resistance is problematic for the effective management of agriculturally important insects, including Metopolophium dirhodum, which is an aphid that commonly feeds on cereals. The insecticide sensitivity status and potential resistance of M. dirhodum field populations remain relatively unknown. In this study, the susceptibility of 19 M. dirhodum populations from seven provinces in Northern China to neonicotinoids, pyrethroids, organophosphates, and a macrolide (abamectin) was determined in 2017–2019. The results indicated that two populations were highly resistant to thiamethoxam, with a relative resistance ratio (RLR) of 134.03 and 103.03, whereas one population was highly resistant to beta-cypermethrin (RLR of 121.42). On the basis of the RLR, the tested M. dirhodum populations ranging from susceptible to showing moderate levels of resistance to imidacloprid (RLR of 1.50 to 57.29), omethoate (RLR of 1.07 to 18.73), and abamectin (RLR of 1.10 to 25.89), but they were ranging from susceptible to showing tolerance or low levels of resistance to bifenthrin (RLR of 1.14 to 6.02) and chlorpyrifos (RLR of 1.11 to 7.59). Furthermore, a pair-wise correlation analysis revealed a significant correlation between the median lethal concentrations (LC50) for beta-cypermethrin and thiamethoxam, reflecting the cross-resistance between these two insecticides. The data obtained in our study provide timely information about aphid insecticide sensitivity, which may be used to delay the evolution of M. dirhodum insecticide resistance in Northern China.

show abstract

“…However, the frequent application and over-reliance on chemical insecticides resulted in the rapid evolution of resistance. Recent reports show that aphids have resistance to most commonly used insecticides in the field [5,6].…”

Section: Introductionmentioning

confidence: 99%

Sublethal and Transgenerational Effects of Nitenpyram on <em>Acyrthosiphon gossypii </em>and Its Impacts on Symbiotic Bacteria

Wei,

Li,

Chen

et al. 2024

Preprint

View full text Add to dashboard Cite

Mutualism between insects and symbiotic bacteria drives insect evolution, and insecticide resistance is affected by symbiotic bacteria, increasing the complexity of pest resistance. This study evaluated the sublethal and transgenerational effects of the neonicotinoid insecticide nitenpyram on the population dynamics of Acyrthosiphon gossypii and measured its impact on the symbiotic bacterial community. The results showed that LC20 (2.49 mg·L−1) of nitenpyram significantly reduced the fecundity and lifespan of the parent G0 generation of A. gossypii, and had similar inhibitory effects on its G1 and G2 generations. Compared with those of control group, the population parameters λ (finite rate of increase), R0 (net re-productive rate), and r (intrinsic increase rate) of G1 and G2 were significantly reduced, but mean gen-eration time (T) was increased. Nitenpyram treatment affected the biological traits of A. gossypii, and also changed the relative abundance of Buchnera and Sphingomonas in three consecutive generations G0-G2. Under nitenpyram treatment, the relative abundance of Buchnera decreased in G0, while that of Sphin-gomonas increased. The relative abundance of Buchnera increased in G1 and G2, but that of Sphingo-monas decreased. Our findings may provide a new perspective for evaluating the potential effects of pesticides on aphids.

show abstract

Enzymes mediating resistance to chlorpyriphos in Aphis fabae (Homoptera: Aphididae)

Cited by 9 publications

References 31 publications

Uridine Diphosphate-Glycosyltransferase RpUGT344D38 Contributes to λ-Cyhalothrin Resistance in Rhopalosiphum padi

Uridine Diphosphate-Glycosyltransferase RpUGT344D38 Contributes to λ-Cyhalothrin Resistance in Rhopalosiphum padi

The Sensitivity of Field Populations of Metopolophium dirhodum (Walker) (Hemiptera: Aphididae) to Seven Insecticides in Northern China

Sublethal and Transgenerational Effects of Nitenpyram on <em>Acyrthosiphon gossypii </em>and Its Impacts on Symbiotic Bacteria

Contact Info

Product

Resources

About